anti-Mouse (Murine) AKT antibody for Flow Cytometry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • beta-actin
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • actin beta
  • CG4006 gene product from transcript CG4006-RE
  • Actin 5C
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • Act5C
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
AA 2-480, pan
222
132
113
66
56
51
37
36
22
22
20
20
18
18
16
13
12
12
12
11
11
10
10
9
9
9
8
7
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1256
667
502
63
58
57
46
35
33
30
18
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Mouse
937
383
12
4
2
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
55
43
41
39
34
26
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
1089
560
479
256
186
175
129
85
67
33
26
15
13
11
11
4
3
3
3
3
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4899444
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.098416 ABIN4900619 CyTOF FACS IHC SimWes WB Rabbit IgG pSer473 Log in to see Polyclonal 12
14.098416 ABIN4896124 FACS PE Mouse IgG2b AA 2-480, pan Log in to see 281046
14.098416 ABIN4896123 FACS APC Mouse IgG2b AA 2-480, pan Log in to see 281046
14.098416 ABIN4898083 FACS Alexa Fluor 488 Mouse IgG1 pSer473 Log in to see 545007
14.098416 ABIN4898084 FACS PE Mouse IgG1 pSer473 Log in to see 545007
14.098416 ABIN4278999 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
14.098416 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6 1
14.098416 ABIN3029453 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 428-457 Log in to see Polyclonal
14.098416 ABIN5542263 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10
1 ABIN950329 EIA FACS IF IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 4
1 ABIN655915 FACS IF IHC (p) WB Rabbit Ig AA 428-457, C-Term Log in to see Polyclonal 1
1 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
1 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
1 ABIN1981392 FACS IF IHC WB Rabbit IgG AA 428-457, C-Term Log in to see Polyclonal
1 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11
1 ABIN2455515 FACS IF IHC WB Mouse IgG1 Log in to see 1G5
1 ABIN2455508 FACS IF IHC WB Mouse IgG1 Log in to see 4D6
1 ABIN2455509 FACS IF IHC WB Mouse IgG1 Log in to see 3E3
1 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4
1 ABIN2455511 FACS IF IHC WB Mouse IgG1 Log in to see 3B4

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 2-480, pan
(222), (132), (113), (66), (56), (51), (37), (36), (22), (22), (20), (20), (18), (18), (16), (13), (12), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1256), (667), (502), (63), (58), (57), (46), (35), (33), (30), (18), (8), (8), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(937), (383), (12), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(55), (43), (41), (39), (34), (26), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Simple Western (SimWes), Western Blotting (WB)
(1089), (560), (479), (256), (186), (175), (129), (85), (67), (33), (26), (15), (13), (11), (11), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN4899444) Images
Specificity Detects human, mouse and rat Akt in direct ELISAs and Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Akt1, Ser2-Ala480, Accession # P31749
Clone 281046
Isotype IgG2b

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN4899444) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Gene ID 207
UniProt P31749
Research Area Kinases/Phosphatases, Cell Cycle, Apoptosis/Necrosis, Cancer
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN4899444) Images back to top
Application Notes Western Blot 0.2 μg/mL
Simple Western 2 μg/mL
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN4899444) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-AKT antibody (ABIN4899444)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Tolvanen, Dash, Polianskyte-Prause, Dumont, Lehtonen: "Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes." in: Journal of cell science, Vol. 128, Issue 24, pp. 4588-600, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Bifulco, Longanesi-Cattani, Franco, Pavone, Mugione, Di Carluccio, Masucci, Arra, Pirozzi, Stoppelli, Carriero: "Single amino acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion." in: PLoS ONE, Vol. 7, Issue 9, pp. e44806, 2012 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Deng, Han, Yan, Tian, Tao, Kang, Li: "Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt." in: Apoptosis : an international journal on programmed cell death, Vol. 15, Issue 4, pp. 463-73, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Han, Wu, Deng, Tao, Guo, Tian, Kang, Zhang, Yan: "Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation." in: Journal of molecular and cellular cardiology, Vol. 48, Issue 6, pp. 1225-35, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN4899444) back to top
Supplier Images
Simple Western (SimWes) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 2-480), (pan) antibody (ABIN4899444) Detection of Human Akt by Simple WesternTM. Simple Western lane view shows lysates of...
Immunocytochemistry (ICC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 2-480), (pan) antibody (ABIN4899444) Akt (pan) in 293T Human Cell Line. Akt pan specific (panels A and C) and Akt1 (E17K M...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 2-480), (pan) antibody (ABIN4899444) Detection of Human Akt Pan Specific by Western Blot. Western blot shows Recombinant H...
Immunocytochemistry (ICC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 2-480), (pan) antibody (ABIN4899444) Akt in MDA-MB-231 Human Cell Line. Akt was detected in immersion fixed MDA-MB-231 hum...
Flow Cytometry (FACS) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 2-480), (pan) antibody (ABIN4899444) Detection of Akt in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cance...
Simple Western (SimWes) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 2-480), (pan) antibody (ABIN4899444) Detection of Human Akt by Simple WesternTM. Simple Western lane view shows lysates of...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 2-480), (pan) antibody (ABIN4899444) Detection of Human/Mouse Akt by Western Blot. Western blot shows lysates of MCF-7 hum...