anti-Mouse (Murine) AKT antibody for Immunofluorescence

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pSer473
187
123
85
65
56
39
32
30
28
22
21
21
18
18
17
14
13
12
12
11
11
10
10
9
9
9
8
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1136
636
493
63
58
56
46
35
33
30
18
8
8
5
5
5
3
3
3
2
1
1
1
1
Host
Rabbit
855
323
11
4
2
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
48
38
35
35
26
25
12
12
7
7
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
964
527
435
219
159
149
116
67
56
33
26
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN264497
$ 830.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.071381 ABIN655915 FACS IF IHC (p) WB Rabbit Ig AA 428-457, C-Term Log in to see Polyclonal 1
12.071381 ABIN950329 EIA FACS IF IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 4
12.071381 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
12.071381 ABIN1532436 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
12.071381 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
12.071381 ABIN968205 IF IP IHC WB Mouse IgG1 AA 71-184 Log in to see 7-Akt-PKBalpha 5
12.071381 ABIN968227 IF WB Mouse IgG2a AA 1-480 Log in to see 2-PKBa-Akt 2
12.071381 ABIN968206 IF IP IHC WB Mouse IgG1 AA 71-184 Log in to see 7-Akt-PKBalpha 5
12.071381 ABIN968218 IF IP IHC WB Mouse IgG1, kappa Log in to see 55-PKBa-Akt 5
12.071381 ABIN968217 IF IP IHC WB Mouse IgG1, kappa Log in to see 55-PKBa-Akt 2
12.071381 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11
12.071381 ABIN2455515 FACS IF IHC WB Mouse IgG1 Log in to see 1G5
12.071381 ABIN615022 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
12.071381 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4
12.071381 ABIN2455511 FACS IF IHC WB Mouse IgG1 Log in to see 3B4
12.071381 ABIN2455509 FACS IF IHC WB Mouse IgG1 Log in to see 3E3
12.071381 ABIN2455508 FACS IF IHC WB Mouse IgG1 Log in to see 4D6
12.071381 ABIN1531419 IF IHC ELISA WB Rabbit IgG pSer246 Log in to see Polyclonal
12.071381 ABIN1869963 IF IHC WB Rabbit IgG pThr450 Log in to see Polyclonal
12.071381 ABIN1869972 IF IHC WB Rabbit IgG pThr308 Log in to see Polyclonal

Top referenced anti-AKT antibody for Immunofluorescence

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Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies 12 Antibodies 11 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Simple Western (SimWes) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 908 Antibodies 543 Antibodies 422 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies 1 Antibodies 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Luminex Assay (LMNX) 3 Antibodies
Intracellular Staining (ICS) 11 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473
(187), (123), (85), (65), (56), (39), (32), (30), (28), (22), (21), (21), (18), (18), (17), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1136), (636), (493), (63), (58), (56), (46), (35), (33), (30), (18), (8), (8), (5), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(855), (323), (11), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(48), (38), (35), (35), (26), (25), (12), (12), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(964), (527), (435), (219), (159), (149), (116), (67), (56), (33), (26), (15), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN264497) Images
Specificity This Antibody reacts to AKT.
Characteristics Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase
Purification Protein A/G Chromatography
Immunogen Chemically synthesized phosphopeptide derived from the region of human Akt thatcontains serine 473.
Clone 14-06-14
Isotype IgG

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN264497) Images back to top
Antigen
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background Akt, also known as Protein Kinase B (PKB) or RAC-α, is a 65 kDa serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating many proteins including GSK-3β, caspase 9, BAD and the Forkhead Transcription Factor. Akt is activated by PI3K, which in turn can interact with proteins such as FAK (when FAK is phosphorylated at tyrosine 397) thereby linking activation to the cytoskeleton. Akt is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2. Phosphorylation at serine 473 is required for full activation of Akt.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Molecular Weight 65 kDa
Gene ID 207
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling ProductDetails: References for anti-AKT antibody (ABIN264497) Images back to top
Application Notes Western Blot: 1: 250. Immunofluorecence: 1: 100. Positive Control: Mouse NIH3T3 cells stimulated with PDGF, 3T3-L1 adipocytes stimulatedwith OSM and IFN-γ.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details ProductDetails: References for anti-AKT antibody (ABIN264497) Images back to top
Buffer PBS, pH ~7.3, 0.05 % Sodium Azide, 1.0 mg/mL BSA, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment The antibody can be shipped on gel ice. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

ProductDetails: References for anti-AKT antibody (ABIN264497)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Yu, Watanabe, Shibuya, Miura: "Redundancy of radioresistant signaling pathways originating from insulin-like growth factor I receptor." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 6702-9, 2003

Welch, Plank, Witt, Glascock, Schaefer, Chimenti, Andreoli, Limana, Leri, Kajstura, Anversa, Sussman: "Cardiac-specific IGF-1 expression attenuates dilated cardiomyopathy in tropomodulin-overexpressing transgenic mice." in: Circulation research, Vol. 90, Issue 6, pp. 641-8, 2002

Beffert, Morfini, Bock, Reyna, Brady, Herz: "Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49958-64, 2002

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN264497) back to top
Supplier Images
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN264497) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody