anti-Rabbit AKT antibody for Western Blotting

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
AA 445-480
188
123
85
65
56
39
32
30
28
22
21
21
18
18
17
14
12
12
12
11
11
10
10
9
9
9
8
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rabbit, Rat (Rattus)
1136
637
493
63
58
56
46
35
33
30
18
8
8
5
5
4
3
3
3
2
1
1
1
1
Host
Mouse
856
322
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
48
38
35
35
26
25
12
12
7
7
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
964
527
435
220
158
148
116
67
56
33
25
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN725180
$ 295.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2972655 IF/ICC IHC WB Rabbit Center, pSer246 Log in to see Polyclonal
1 ABIN2972759 WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2972623 WB Rabbit Center Log in to see Polyclonal
1 ABIN2972757 WB Rabbit C-Term, pThr450 Log in to see Polyclonal

Similar anti-AKT Antibodies

Application / Reactivity Rat (Rattus)
Dot Blot (DB) 1 Antibodies
ELISA 171 Antibodies
Enzyme Immunoassay (EIA) 20 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 49 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 107 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 219 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 91 Antibodies
Immunoprecipitation (IP) 58 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 445-480
(188), (123), (85), (65), (56), (39), (32), (30), (28), (22), (21), (21), (18), (18), (17), (14), (12), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rabbit, Rat (Rattus)
(1136), (637), (493), (63), (58), (56), (46), (35), (33), (30), (18), (8), (8), (5), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(856), (322), (11), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(48), (38), (35), (35), (26), (25), (12), (12), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(964), (527), (435), (220), (158), (148), (116), (67), (56), (33), (25), (15), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human AKT1
Isotype IgG

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

Subcellular location: Cytoplasm, Nucleus, Cell membrane


Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes WB(1:100-1000), FCM(1:20-100), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 445-480) (ABIN725180) Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PKB Polyclon...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 445-480) (ABIN725180) Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PKB Polyclon...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 445-480) (ABIN725180) Mouse pancreas lysates probed with AKT1/3 Polyclonal Antibody, unconjugated at 1:300...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 445-480) (ABIN725180) Mouse placenta lysates probed with Akt1/3 Polyclonal Antibody, unconjugated at 1:300...