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anti-Rat (Rattus) AKT antibody for Intracellular Staining

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
1064
472
380
43
41
30
30
20
17
15
10
6
5
4
2
2
1
1
1
Host
Mouse
878
207
14
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is conjugated to PE
40
33
32
32
27
25
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
880
516
414
201
109
107
107
71
42
36
24
11
11
6
3
3
3
3
1
1
1
1
1
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Catalog No. ABIN1176873
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00086993724 ABIN1176875 ICS Alexa Fluor 488 Mouse IgG1, kappa Log in to see 55-PKBa-Akt 5
0.00086993724 ABIN1176874 ICS Alexa Fluor 647 Mouse IgG1, kappa Log in to see 55-PKBa-Akt 5

Top referenced anti-AKT antibody for Intracellular Staining

Similar anti-AKT Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 850 Antibodies 404 Antibodies 330 Antibodies 27 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies 1 Antibodies 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Luminex Assay (LMNX) 3 Antibodies
Intracellular Staining (ICS) 12 Antibodies 12 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 98 Antibodies 53 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(1064), (472), (380), (43), (41), (30), (30), (20), (17), (15), (10), (6), (5), (4), (2), (2), (1), (1), (1)
Host Mouse
(878), (207), (14), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is conjugated to PE
(40), (33), (32), (32), (27), (25), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(880), (516), (414), (201), (109), (107), (107), (71), (42), (36), (24), (11), (11), (6), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1176873) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Akt1 Recombinant Protein
Clone 55-PKBa-Akt
Isotype IgG1, kappa

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN1176873) Images back to top
Antigen
Alternative Name Akt1 (AKT1 Antibody Abstract)
Background Akt [also known as PKB (Protein kinase B) or RAC-PK (Related to the A and C kinases] is a family of serine/threonine kinases that contains a pleckstrin homology (PH) domain. PH domains play important roles in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (alpha), Akt2 (beta) and Akt3 (gamma)], they are thought to be regulated similarly. Akt is activated by insulin and growth factors by a mechanism involving phosphoinositide 3-OH kinase. Phosphoinositide 3-OH kinase products bind to the PH domain, resulting in translocation of Akt to the plasma membrane and activation of Akt to phospho-Akt by upstream kinases. Akt is phosphorylated within the activation loop at threonine 308 and the C-terminus at serine 473. Phospho-Akt promotes cell survival by inhibiting apoptosis. Specifically, phospho-Akt1 has been shown to phosphorylate Bad, a member of the Bcl-2 family that promotes cell death. This phosphorylation results in the inactivation of the proapoptotic function of Bad. The Akt molecule is thus considered to link extracellular survival signals (growth factors) with the apoptotic machinery (Bad). Akt is also a key mediator of the metabolic effects of insulin. Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors. The 55/PKBa/Akt monoclonal antibody recognizes Akt1, regardless of phosphorylation status. The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.
Synonyms: PKBalpha, RAC-PK
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN1176873) Images back to top
Application Notes Either BD Cytofix™ fixation buffer or BD Phosflow™ Fix Buffer I may be used for cell fixation.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN1176873) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

References for anti-AKT Antibody (ABIN1176873)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999

Ferrigno, Silver: "Regulated nuclear localization of stress-responsive factors: how the nuclear trafficking of protein kinases and transcription factors contributes to cell survival." in: Oncogene, Vol. 18, Issue 45, pp. 6129-34, 1999

Kandel, Hay: "The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB." in: Experimental cell research, Vol. 253, Issue 1, pp. 210-29, 1999

Alessi, Andjelkovic, Caudwell, Cron, Morrice, Cohen, Hemmings: "Mechanism of activation of protein kinase B by insulin and IGF-1." in: The EMBO journal, Vol. 15, Issue 23, pp. 6541-51, 1997

Datta, Dudek, Tao, Masters, Fu, Gotoh, Greenberg: "Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery." in: Cell, Vol. 91, Issue 2, pp. 231-41, 1997