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|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AKT antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN264497) Images
|Specificity||This Antibody reacts to AKT.|
|Characteristics||Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase|
|Purification||Protein A/G Chromatography|
|Immunogen||Chemically synthesized phosphopeptide derived from the region of human Akt thatcontains serine 473.|
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN264497) Images back to top
|Alternative Name||AKT1 / PKB (AKT1 Antibody Abstract)|
|Background||Akt, also known as Protein Kinase B (PKB) or RAC-α, is a 65 kDa serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating many proteins including GSK-3β, caspase 9, BAD and the Forkhead Transcription Factor. Akt is activated by PI3K, which in turn can interact with proteins such as FAK (when FAK is phosphorylated at tyrosine 397) thereby linking activation to the cytoskeleton. Akt is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2. Phosphorylation at serine 473 is required for full activation of Akt.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase|
|Molecular Weight||65 kDa|
|Research Area||Signaling, Protein Modifications, Kinases/Phosphatases|
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling ProductDetails: References for anti-AKT antibody (ABIN264497) Images back to top
Western Blot: 1: 250. Immunofluorecence: 1: 100. Positive Control: Mouse NIH3T3 cells stimulated with PDGF, 3T3-L1 adipocytes stimulatedwith OSM and IFN-γ.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details ProductDetails: References for anti-AKT antibody (ABIN264497) Images back to top
|Buffer||PBS, pH ~7.3, 0.05 % Sodium Azide, 1.0 mg/mL BSA, 50 % Glycerol|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody can be shipped on gel ice. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ProductDetails: References for anti-AKT antibody (ABIN264497)Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Yu, Watanabe, Shibuya, Miura: "Redundancy of radioresistant signaling pathways originating from insulin-like growth factor I receptor." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 6702-9, 2003
Welch, Plank, Witt, Glascock, Schaefer, Chimenti, Andreoli, Limana, Leri, Kajstura, Anversa, Sussman: "Cardiac-specific IGF-1 expression attenuates dilated cardiomyopathy in tropomodulin-overexpressing transgenic mice." in: Circulation research, Vol. 90, Issue 6, pp. 641-8, 2002
Beffert, Morfini, Bock, Reyna, Brady, Herz: "Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49958-64, 2002
ImagesProduct Details anti-AKT Antibody Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN264497) back to top