Human AKT ELISA Kit

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Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA Kits
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
32
30
24
4
1
1
Method Type
Sandwich ELISA
Detection Range
0.156-10 ng/mL
Minimum Detection Limit
0.156 ng/mL
Application
ELISA
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Catalog No. ABIN1571880
$ 908.41
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.70292 ABIN810549 Sandwich ELISA Serum, Plasma, Cell Lysate 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN625220 Sandwich ELISA Cell Lysate Log in to see 1
1 ABIN625233 Sandwich ELISA Cell Lysate Log in to see 1
1 ABIN5591538 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.78-50 ng/mL 0.78 ng/mL Log in to see
1 ABIN456280 Sandwich ELISA Serum, Tissue Homogenate, Biological Fluids 0.78-50 ng/mL 0.78 ng/mL Log in to see
1 ABIN4885966 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN5519721 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN1380362 Cell ELISA Log in to see
1 ABIN1380871 Cell ELISA Log in to see
1 ABIN1380516 Cell ELISA Log in to see
1 ABIN1380284 Cell ELISA Log in to see
1 ABIN1380285 Cell ELISA Log in to see
1 ABIN1380287 Cell ELISA Log in to see
1 ABIN1380154 Cell ELISA Log in to see
1 ABIN4881275 Cell ELISA Cell Culture Cells Log in to see
1 ABIN2967306 Sandwich ELISA Plasma, Serum, Tissue Samples, Biological Fluids 1.563-100 ng/mL 1.563 ng/mL Log in to see
1 ABIN2065173 Cell ELISA Log in to see
1 ABIN2065188 Cell ELISA Log in to see
1 ABIN2065185 Cell ELISA Log in to see
1 ABIN2065178 Cell ELISA Log in to see

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General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(32), (30), (24), (4), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.156-10 ng/mL
Minimum Detection Limit 0.156 ng/mL
Application ELISA
Supplier Log in to see

Product Details AKT ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PKBa in human tissue homogenates, cell lysates and other biological fluids.
Sample Type Biological Fluids, Cell Lysate, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.053 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Target details

Product Details AKT ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Protein Kinase B Alpha (PKBa) (AKT1 ELISA Kit Abstract)
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details AKT ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Assay Procedure The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Restrictions For Research Use only

Handling

Product Details AKT ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months

Images

Product Details AKT ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for AKT ELISA Kit (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (ABIN1571880) V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA Kit