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anti-Chicken AKT2 antibody for Enzyme Immunoassay

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • 2410016A19Rik
  • AKT2
  • akt2-a
  • AW554154
  • cb945
  • HIHGHH
  • PKB
  • PKBB
  • pkbb
  • PKBBETA
  • PKBbeta
  • pkbbeta
  • pkbss
  • prkbb
  • PRKBB
  • RAC-BETA
  • rac-beta
  • v-akt2
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
386
122
98
29
27
24
22
20
20
18
18
18
18
1
1
1
Host
Rabbit
300
70
12
10
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
333
219
144
80
42
25
25
21
19
11
10
4
4
3
1
1
1
Supplier
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Catalog No. ABIN264873
$ 357.50
Plus shipping costs $45.00

Top referenced anti-AKT2 antibody for Enzyme Immunoassay

Similar anti-AKT2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Chicken Guinea Pig Hamster Monkey Pig (Porcine) Rabbit Sheep (Ovine) Xenopus laevis
Western Blotting (WB) 327 Antibodies 105 Antibodies 86 Antibodies 23 Antibodies 28 Antibodies 23 Antibodies 19 Antibodies 19 Antibodies 28 Antibodies 21 Antibodies 19 Antibodies 19 Antibodies 21 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Immunoprecipitation (IP) 22 Antibodies 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
(386), (122), (98), (29), (27), (24), (22), (20), (20), (18), (18), (18), (18), (1), (1), (1)
Host Rabbit
(300), (70), (12), (10), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(333), (219), (144), (80), (42), (25), (25), (21), (19), (11), (10), (4), (4), (3), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN264873) Images
Specificity This antibody detects a ~65 kDa protein, corresponding to the Molecular Mass of Akt2 PKB beta on SDS-PAGE immunoblots. Does not react with PKB-alpha (Akt1) or PKB-gamma (Akt3).
Purification Affinity Chromatography
Immunogen A five residue synthetic peptide based on the Human Akt2, coupled to KLH
Isotype IgG

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 Antibody (ABIN264873) Images back to top
Antigen
Alternative Name AKT2 / PKB beta (AKT2 Antibody Abstract)
Background Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
Gene ID 208
NCBI Accession NP_001617
UniProt P31751
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 Antibody (ABIN264873) Images back to top
Application Notes Western blot (3): 1: 13000 dilution of SPC-148 was ufficient for detection of PKBβ in 20 μg ofHeLa cell lysate by ECL immunoblot analysis. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 Antibody (ABIN264873) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS, 0.09 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-AKT2 Antibody (ABIN264873)

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Background publications

Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002

Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001

Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN264873) back to top
Supplier Images
 image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (ABIN264873) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody