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anti-Human AKT2 antibody for Immunofluorescence

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • 2410016A19Rik
  • AKT2
  • akt2-a
  • AW554154
  • cb945
  • HIHGHH
  • PKB
  • PKBB
  • pkbb
  • PKBBETA
  • PKBbeta
  • pkbbeta
  • pkbss
  • prkbb
  • PRKBB
  • RAC-BETA
  • rac-beta
  • v-akt2
Reactivity
Human, Mouse (Murine)
386
122
99
30
28
25
23
21
21
19
19
19
19
1
1
1
Host
Mouse
301
69
12
10
3
2
Clonality
Monoclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
333
219
144
79
41
25
25
21
19
11
11
4
4
3
1
1
1
Supplier
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Catalog No. ABIN659024
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31524822 ABIN1105276 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1B6 1
0.31524822 ABIN513224 IF ELISA WB Mouse IgG1 kappa AA 100-189, partial Log in to see 1D9 1
0.31524822 ABIN2704466 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.31524822 ABIN614384 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1B6
0.31524822 ABIN2159040 FACS IF IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.31524822 ABIN2159036 IF IHC WB Mouse IgG1 Log in to see 148CT5-4-1-4
0.31524822 ABIN2159038 FACS IF IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.31524822 ABIN2167708 IF IHC ELISA WB Mouse IgG2b Log in to see 1B6
0.31524822 ABIN1585952 IF WB Mouse IgG2b Log in to see 1B6
0.31524822 ABIN950116 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 100-189, partial Log in to see X1
0.31524822 ABIN559837 IF ELISA WB Mouse IgG1 kappa AA 100-189, partial Log in to see 1B3
0.31524822 ABIN513226 IF ELISA WB Mouse IgG1 kappa AA 100-189, partial Log in to see 1F3
0.001088782 ABIN391022 FACS IF IHC (p) WB Rabbit Ig AA 416-444, C-Term Log in to see Polyclonal 1
0.001088782 ABIN391024 IF IHC (p) WB Rabbit Ig AA 452-481, Ser474 Log in to see Polyclonal 1
0.001088782 ABIN391021 FACS IF IHC (p) WB Rabbit Ig AA 93-123, N-Term Log in to see Polyclonal 1
0.001088782 ABIN391023 FACS IF IHC (p) WB Rabbit Ig AA 93-123, Center Log in to see Polyclonal 2
0.001088782 ABIN393457 IF WB Mouse IgG1 kappa AA 100-189 Log in to see 1B3 5
0.001088782 ABIN393501 IF WB Mouse IgG1 kappa AA 100-189 Log in to see 1D9 5
0.001088782 ABIN652284 FACS IF WB Rabbit Ig Log in to see Polyclonal 2
0.001088782 ABIN393514 IF WB Mouse IgG1 kappa AA 100-189 Log in to see 1F3 5

Top referenced anti-AKT2 antibody for Immunofluorescence

Similar anti-AKT2 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 40 Antibodies 210 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 11 Antibodies
Flow Cytometry (FACS) 6 Antibodies 25 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 31 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 56 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 105 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 19 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Reactivity Human, Mouse (Murine)
(386), (122), (99), (30), (28), (25), (23), (21), (21), (19), (19), (19), (19), (1), (1), (1)
Host Mouse
(301), (69), (12), (10), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(333), (219), (144), (79), (41), (25), (25), (21), (19), (11), (11), (4), (4), (3), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN659024) Images
Specificity This AKT2 monoclonal antibody is generated from mouse immunized with AKT2 recombinant protein.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Immunogen This AKT2 monoclonal antibody is generated from mouse immunized with AKT2 recombinant protein.
Clone 148CT5-4-1-4
Isotype IgG1

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 Antibody (ABIN659024) Images back to top
Antigen
Alternative Name AKT2 (AKT2 Antibody Abstract)
Background AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Synonyms: Protein kinase B beta, PKB beta, Protein kinase Akt-2, RAC-PK-beta, RAC-beta serine/threonine-protein kinase,AKT2
Pathways: Neurotrophin signaling pathway, Hepatitis C, Insulin signaling pathway, Adipocytokine signaling pathway, Jak-STAT signaling pathway, Fc gamma R-mediated phagocytosis, MAPK signaling pathway, VEGF signaling pathway, Fc epsilon RI signaling pathway, Toxoplasmosis, Non-small cell lung cancer, Chemokine signaling pathway, Toll-like receptor signaling pathway, Measles, Tuberculosis, Focal adhesion, T cell receptor signaling pathway, B cell receptor signaling pathway, Prostate cancer, Melanoma, Tight junction, Small cell lung cancer, Renal cell carcinoma, mTOR signaling pathway, Apoptosis, Chagas disease (American trypanosomiasis), Colorectal cancer, Pancreatic cancer, Osteoclast differentiation, ErbB signaling pathway, Chronic myeloid leukemia, Endometrial cancer, Carbohydrate digestion and absorption, Acute myeloid leukemia, Glioma
Molecular Weight 55769 Da
Gene ID 208
UniProt P31751
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 Antibody (ABIN659024) Images back to top
Application Notes WB = 1:100-500, IHC (p) = 1:50-100, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 Antibody (ABIN659024) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-AKT2 Antibody (ABIN659024)

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Product cited in:

Sun, Zhang, Wang, Jin, Jia, Yu, Chen: "MiRNA-615-5p functions as a tumor suppressor in pancreatic ductal adenocarcinoma by targeting AKT2." in: PLoS ONE, Vol. 10, Issue 4, pp. e0119783, 2015

Cui, Lin, Zuo, Zhang, Dong, Hu, Luo, Chen, Lu: "AKT2-knockdown suppressed viability with enhanced apoptosis, and attenuated chemoresistance to temozolomide of human glioblastoma cells in vitro and in vivo." in: OncoTargets and therapy, Vol. 8, pp. 1681-90, 2015

Background publications

Fortier, Van Themsche, Asselin, Cadrin: "Akt isoforms regulate intermediate filament protein levels in epithelial carcinoma cells." in: FEBS letters, Vol. 584, Issue 5, pp. 984-8, 2010

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN659024) back to top
Supplier Images
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (ABIN659024) AKT2 antibody western blot analysis in mouse brain tissue lysates (15ug/lane).This d...
Immunohistochemistry (IHC) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (ABIN659024) AKT2 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffi...
Immunofluorescence (IF) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (ABIN659024) Confocal immunofluorescent analysis of AKT2 Antibody with Hela cell followed by Alex...