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|Application / Reactivity||Human|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies|
|Dot Blot (DB)||19 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||11 Antibodies|
|Flow Cytometry (FACS)||34 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||30 Antibodies|
|Immunofluorescence (IF)||83 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||143 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||48 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies|
|Luminex Assay (LMNX)||4 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||335 Antibodies|
|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies|
|Conjugate||This AKT2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-AKT2 AntibodyTarget Details AKT2 Application Details Handling Images
|Specificity||This antibody specifically recognizes the beta isoform of PKB (PKBβ/Akt2).|
|Characteristics||Synonyms: RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta, RAC-betaserine/threonine-protein kinase|
|Purification||Thiophilic Adsorption and Size Exclusion Chromatography.|
|Components||incl. positive Control|
|Immunogen||Synthetic peptide conjugated to KLH|
Target Details AKT2Product Details anti-AKT2 Antibody Application Details Handling Images back to top
|Alternative Name||AKT2 / PKB beta (AKT2 Antibody Abstract)|
|Background||Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers. AKT2 encodes a RAC/AKT-type protein kinase that contains a N-terminal pleckstrin-homology (PH) domain and a central catalytic domain closely related to both cAMP-dependent protein kinase and protein kinase C. The protein is a member of PI3K-mediated signalling pathways associated with the regulation of proliferation, survival, protein synthesis, and metabolism. It is activated by a variety of growth factors. AKT2 has been shown to be transcriptionally regulated by MyoD and to activate MyoD-myocyte enhancer binding factor-2 (MEF2) transactivation activity during muscle differentiation. Glycogen synthase kinase 3 (GSK-3) also has been shown to be a downstream target of AKT2. The AKT2 gene is one of the human homologues of v-akt, the transduced oncogene of the AKT8 virus, which induces lymphomas in mice. It has been implicated in breast, ovarian, and pancreatic cancers.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling|
Application DetailsProduct Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL, 2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling back to top