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anti-Human AKT2 antibody for Immunohistochemistry (Frozen Sections)

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • AKT2
  • akt2
  • Akt2
Epitope
AA 454-481, C-Term
55
39
31
22
20
15
12
12
9
9
7
6
5
5
5
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
416
167
127
38
34
29
27
25
24
23
22
22
22
2
2
2
2
1
1
1
Host
Rabbit
306
86
12
11
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
351
222
151
85
51
32
31
27
18
13
11
7
4
3
2
2
2
1
Options
Supplier
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Catalog No. ABIN3043781
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126856 EIA IHC (fro) WB Mouse IgG2a Log in to see 8B7
1 ABIN3029473 IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-AKT2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 2 Antibodies 2 Antibodies
ELISA 47 Antibodies 62 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 3 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 53 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 111 Antibodies
Flow Cytometry (FACS) 6 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Epitope AA 454-481, C-Term
(55), (39), (31), (22), (20), (15), (12), (12), (9), (9), (7), (6), (5), (5), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(416), (167), (127), (38), (34), (29), (27), (25), (24), (23), (22), (22), (22), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(306), (86), (12), (11), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(351), (222), (151), (85), (51), (32), (31), (27), (18), (13), (11), (7), (4), (3), (2), (2), (2), (1)
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling Images
Sequence DRYDSLGLLE LDQRTHFPQF SYSASIRE
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for RAC-beta serine/threonine-protein kinase(AKT2) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: v-akt murine thymoma viral oncogene homolog 2
Protein Name: RAC-beta serine/threonine-protein kinase
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human AKT2(454-481aa DRYDSLGLLELDQRTHFPQFSYSASIRE), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.
Isotype IgG

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT2 (AKT2 Antibody Abstract)
Background AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. This gene is mapped to 19q13.2. AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.
Gene ID 208
UniProt P31751
Research Area Cardiovascular, Atherosclerosis, Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for AKT2 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (AA 454-481) (ABIN3043781) Observed bind size: 56KD
Immunohistochemistry (IHC) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (AA 454-481) (ABIN3043781) Anti-AKT2 Picoband antibody, IHC(P): Human Intestinal Cancer Tissue
Immunohistochemistry (IHC) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (AA 454-481) (ABIN3043781) Anti-AKT2 Picoband antibody, IHC(P): Human Lung Cancer Tissue