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anti-Human AKT2 antibody for Immunohistochemistry (Frozen Sections)

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • 2410016A19Rik
  • AKT2
  • akt2-a
  • AW554154
  • cb945
  • HIHGHH
  • PKB
  • PKBB
  • pkbb
  • PKBBETA
  • PKBbeta
  • pkbbeta
  • pkbss
  • prkbb
  • PRKBB
  • RAC-BETA
  • rac-beta
  • v-akt2
Reactivity
Human
402
127
104
30
28
25
23
21
21
19
19
19
19
2
1
1
Host
Mouse
305
80
12
11
3
2
Clonality
Monoclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
342
223
149
83
48
34
28
22
19
11
10
4
3
3
1
1
1
Supplier
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Catalog No. ABIN126856
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008770977 ABIN3043781 IHC (fro) IHC (p) WB Rabbit IgG AA 454-481, C-Term Log in to see Polyclonal
0.0008770977 ABIN1860233 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 142-412 Log in to see Polyclonal
0.0008770977 ABIN3029473 IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-AKT2 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 19 Antibodies
ELISA 214 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 34 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 29 Antibodies
Immunofluorescence (IF) 84 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 142 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 23 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 336 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Reactivity Human
(402), (127), (104), (30), (28), (25), (23), (21), (21), (19), (19), (19), (19), (2), (1), (1)
Host Mouse
(305), (80), (12), (11), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(342), (223), (149), (83), (48), (34), (28), (22), (19), (11), (10), (4), (3), (3), (1), (1), (1)
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling Images
Specificity This antibody specifically recognizes the beta isoform of PKB (PKBβ/Akt2).
Characteristics Synonyms: RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta, RAC-betaserine/threonine-protein kinase
Purification Thiophilic Adsorption and Size Exclusion Chromatography.
Components incl. positive Control
Immunogen Synthetic peptide conjugated to KLH
Clone 8B7
Isotype IgG2a

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT2 / PKB beta (AKT2 Antibody Abstract)
Background Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers. AKT2 encodes a RAC/AKT-type protein kinase that contains a N-terminal pleckstrin-homology (PH) domain and a central catalytic domain closely related to both cAMP-dependent protein kinase and protein kinase C. The protein is a member of PI3K-mediated signalling pathways associated with the regulation of proliferation, survival, protein synthesis, and metabolism. It is activated by a variety of growth factors. AKT2 has been shown to be transcriptionally regulated by MyoD and to activate MyoD-myocyte enhancer binding factor-2 (MEF2) transactivation activity during muscle differentiation. Glycogen synthase kinase 3 (GSK-3) also has been shown to be a downstream target of AKT2. The AKT2 gene is one of the human homologues of v-akt, the transduced oncogene of the AKT8 virus, which induces lymphomas in mice. It has been implicated in breast, ovarian, and pancreatic cancers.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
Gene ID 208
UniProt P31751
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling Images back to top
Application Notes Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL, 2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (ABIN126856) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody