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anti-Human AKT2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended AKT2 Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
  • 2410016A19Rik
  • AKT2
  • akt2-a
  • AW554154
  • cb945
  • HIHGHH
  • PKB
  • PKBB
  • pkbb
  • PKBBETA
  • PKBbeta
  • pkbbeta
  • pkbss
  • prkbb
  • PRKBB
  • RAC-BETA
  • rac-beta
  • v-akt2
Epitope
C-Term
54
41
40
30
25
16
11
10
7
6
5
5
4
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
386
123
99
30
28
25
23
21
21
19
19
19
19
1
1
1
Host
Rabbit
300
70
12
10
3
2
Clonality
Polyclonal
Conjugate
This AKT2 antibody is un-conjugated
19
19
18
16
15
15
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
333
219
144
80
41
25
25
21
19
11
10
4
4
3
1
1
1
Supplier
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Catalog No. ABIN359079
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.33929166 ABIN359081 EIA IHC (p) WB Rabbit Ig Ser474 Log in to see Polyclonal 4
0.33929166 ABIN359078 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 5
0.33929166 ABIN498710 IHC (p) WB Rabbit pSer474 Log in to see Polyclonal
0.33929166 ABIN213654 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.33929166 ABIN498587 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008997883 ABIN391022 FACS IF IHC (p) WB Rabbit Ig AA 416-444, C-Term Log in to see Polyclonal 1
0.0008997883 ABIN391024 IF IHC (p) WB Rabbit Ig AA 452-481, Ser474 Log in to see Polyclonal 1
0.0008997883 ABIN391021 FACS IF IHC (p) WB Rabbit Ig AA 93-123, N-Term Log in to see Polyclonal 1
0.0008997883 ABIN391023 FACS IF IHC (p) WB Rabbit Ig AA 93-123, Center Log in to see Polyclonal 2
0.0008997883 ABIN659024 IF IHC (p) WB Mouse IgG1 Log in to see 148CT5-4-1-4 3
0.0008997883 ABIN196826 IHC (p) WB Rabbit pSer474 Log in to see Polyclonal 3
0.0008997883 ABIN197358 IHC (p) WB Rabbit Ser474 Log in to see Polyclonal 2
0.0008997883 ABIN3043781 IHC (fro) IHC (p) WB Rabbit IgG AA 454-481, C-Term Log in to see Polyclonal
0.0008997883 ABIN657827 IHC (p) WB Rabbit Ig AA 93-122, N-Term Log in to see Polyclonal
0.0008997883 ABIN574055 IF IHC (p) ELISA WB Mouse IgG2b Log in to see 1B6
0.0008997883 ABIN302641 IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.0008997883 ABIN1105276 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1B6 1
0.0008997883 ABIN2888354 IHC (p) ELISA WB Rabbit IgG pSer474 Log in to see Polyclonal
0.0008997883 ABIN2888944 IHC (p) ELISA WB Rabbit IgG AA 432-481 Log in to see Polyclonal
0.0008997883 ABIN614384 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1B6

Top referenced anti-AKT2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-AKT2 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 19 Antibodies
ELISA 210 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 25 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 81 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 137 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 327 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) Antibodies
Epitope C-Term
(54), (41), (40), (30), (25), (16), (11), (10), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(386), (123), (99), (30), (28), (25), (23), (21), (21), (19), (19), (19), (19), (1), (1), (1)
Host Rabbit
(300), (70), (12), (10), (3), (2)
Clonality
Polyclonal
Conjugate This AKT2 antibody is un-conjugated
(19), (19), (18), (16), (15), (15), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(333), (219), (144), (80), (41), (25), (25), (21), (19), (11), (10), (4), (4), (3), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-AKT2 Antibody

Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN359079) Images
Specificity This antibody reacts to AKT2.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AKT2.
Isotype Ig

Target Details AKT2

Product Details anti-AKT2 Antibody Application Details Handling References for anti-AKT2 Antibody (ABIN359079) Images back to top
Antigen
Alternative Name AKT2 / PKB beta (AKT2 Antibody Abstract)
Background AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
Gene ID 208, 9606
UniProt P31751
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling

Application Details

Product Details anti-AKT2 Antibody Target Details AKT2 Handling References for anti-AKT2 Antibody (ABIN359079) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details References for anti-AKT2 Antibody (ABIN359079) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-AKT2 Antibody (ABIN359079)

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling Images back to top
Background publications

George, Rochford, Wolfrum, Gray, Schinner, Wilson, Soos, Murgatroyd, Williams, Acerini, Dunger, Barford, Umpleby, Wareham, Davies, Schafer, Stoffel, ORahilly, Barroso: "A family with severe insulin resistance and diabetes due to a mutation in AKT2." in: Science (New York, N.Y.), Vol. 304, Issue 5675, pp. 1325-8, 2004

Shim, Kang, Jeon, Kang, Chang, Kim: "Protein kinase B inhibits apoptosis induced by actinomycin D in ECV304 cells through phosphorylation of caspase 8." in: Archives of biochemistry and biophysics, Vol. 425, Issue 2, pp. 214-20, 2004

Xu, Sakon, Nagano, Hiraoka, Yamamoto, Hayashi, Dono, Nakamori, Umeshita, Ito, Matsuura, Monden: "Akt2 expression correlates with prognosis of human hepatocellular carcinoma." in: Oncology reports, Vol. 11, Issue 1, pp. 25-32, 2003

Li, Leu, Cheong, Zhang, Baibakov, Shih, Birnbaum, Donowitz: "Akt2, phosphatidylinositol 3-kinase, and PTEN are in lipid rafts of intestinal cells: role in absorption and differentiation." in: Gastroenterology, Vol. 126, Issue 1, pp. 122-35, 2003

Vojtek, Taylor, DeRuiter, Yu, Figueroa, Kwok, Turner: "Akt regulates basic helix-loop-helix transcription factor-coactivator complex formation and activity during neuronal differentiation." in: Molecular and cellular biology, Vol. 23, Issue 13, pp. 4417-27, 2003

Images

Product Details anti-AKT2 Antibody Target Details AKT2 Application Details Handling References for anti-AKT2 Antibody (ABIN359079) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (C-Term) (ABIN359079) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 an...
Western Blotting (WB) image for anti-AKT2 antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 2) (C-Term) (ABIN359079) Western blot analysis of AKT2 (arrow) using AKT2 Antibody