Mouse (Murine) AKT2 ELISA Kit

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Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) ELISA Kits
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • AKT2
  • akt2
  • Akt2
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
14
11
6
Method Type
Sandwich ELISA
Detection Range
0.312-20 ng/mL
Minimum Detection Limit
0.312 ng/mL
Application
ELISA
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Catalog No. ABIN5608516
$ 891.06
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN1380517 Cell ELISA Log in to see
1 ABIN1380913 Cell ELISA Log in to see
1 ABIN2114408 Cell ELISA Log in to see
1 ABIN2114407 Cell ELISA Log in to see
1 ABIN2683384 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN1154302 Log in to see

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(14), (11), (6)
Method Type Sandwich ELISA
Detection Range 0.312-20 ng/mL
Minimum Detection Limit 0.312 ng/mL
Application ELISA
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Product Details AKT2 ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PKBb in mouse serum, plasma, tissue homogenates and other biological fluids.
Sample Type Biological Fluids, Plasma, Serum, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Sensitivity 0.122 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Target details

Product Details AKT2 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Protein Kinase B Beta (PKBb) (AKT2 ELISA Kit Abstract)
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling

Application Details

Product Details AKT2 ELISA Kit Target details Handling Images back to top
Assay Time 1 - 4.5 h
Plate Pre-coated
Assay Procedure The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Restrictions For Research Use only

Handling

Product Details AKT2 ELISA Kit Target details Application Details Images back to top
Handling Advice The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Expiry Date 12 months

Images

Product Details AKT2 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) ELISA Kit (ABIN5608516) Typical Standard Curve