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anti-Mouse (Murine) BIM antibody for Immunocytochemistry

Recommended BIM Antibody (supplied by: Log in to see )

Antigen
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
  • bim
  • BAM
  • BIM
  • BOD
  • 1500006F24Rik
  • Bim
  • Bod
  • BimL
  • BIMEL
  • BCL2-like 11
  • BCL2-like 11 (apoptosis facilitator)
  • bcl2l11
  • LOC100345483
  • BCL2L11
  • Bcl2l11
Reactivity
Human, Mouse (Murine), Rat (Rattus)
327
233
206
9
6
6
4
3
3
1
1
Host
Rabbit
277
29
16
9
7
Clonality
Polyclonal
Conjugate
This BIM antibody is un-conjugated
16
14
11
9
9
9
5
4
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
274
136
112
77
68
43
33
28
17
7
5
4
3
2
2
2
2
Options
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Catalog No. ABIN361752
$ 383.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1732263 ICC IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN4284639 ELISA ICC IF WB Mouse IgG1 Log in to see 1C2H4
1 ABIN1001901 ICC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 2
1 ABIN213909 ICC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN4284637 ELISA ICC IF WB Rabbit Log in to see Polyclonal
1 ABIN4284638 ELISA ICC IF WB Mouse IgG1 Log in to see 1C2C8
1 ABIN4284642 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1583588 ICC ELISA WB Mouse IgG1 Log in to see 1C2C8
1 ABIN1584101 ICC ELISA WB Mouse IgG1 Log in to see 1C2H4
1 ABIN205634 ICC FACS IP IHC ELISA WB Rat IgG2a AA 20-40 Log in to see
1 ABIN1100198 ICC WB Rabbit IgG AA 22-40 Log in to see Polyclonal
1 ABIN2617519 ELISA FACS ICC IHC IP WB Rat IgG2a, kappa AA 20-40 Log in to see 10B12
1 ABIN2617520 ELISA FACS ICC IHC IP WB Rat IgG2a, kappa AA 20-40 Log in to see 3C5
1 ABIN1836673 ICC IF ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1031197 ICC ELISA WB Rabbit Middle Region 2 Log in to see Polyclonal
1 ABIN604449 ICC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4284643 ELISA ICC IF WB DyLight 350 Mouse IgG1 Log in to see 1C2H4
1 ABIN4284654 ELISA ICC IF WB Alexa Fluor 647 Mouse IgG1 Log in to see 1C2H4
1 ABIN4284652 ELISA ICC IF WB Alexa Fluor 405 Mouse IgG1 Log in to see 1C2H4
1 ABIN4284649 ELISA ICC IF WB DyLight 488 Mouse IgG1 Log in to see 1C2H4

Top referenced anti-BIM antibody for Immunocytochemistry

Similar anti-BIM Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 40 Antibodies 47 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 4 Antibodies
Flow Cytometry (FACS) 2 Antibodies 13 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 66 Antibodies 75 Antibodies
Immunofluorescence (IF) 50 Antibodies 60 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 28 Antibodies
Immunohistochemistry (IHC) 81 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 167 Antibodies

General

Antigen BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(327), (233), (206), (9), (6), (6), (4), (3), (3), (1), (1)
Host Rabbit
(277), (29), (16), (9), (7)
Clonality
Polyclonal
Conjugate This BIM antibody is un-conjugated
(16), (14), (11), (9), (9), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(274), (136), (112), (77), (68), (43), (33), (28), (17), (7), (5), (4), (3), (2), (2), (2), (2)
Pubmed 2 references available
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Product Details anti-BIM Antibody

Target Details BIM Application Details Handling References for anti-BIM Antibody (ABIN361752) Images
Specificity Detects ~23 kDa.
Purification Protein A Purified
Immunogen Internal central amino acids of human Bim

Target Details BIM

Product Details anti-BIM Antibody Application Details Handling References for anti-BIM Antibody (ABIN361752) Images back to top
Antigen
Alternative Name BIM/BOD (BCL2L11 Antibody Abstract)
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).
Gene ID 10018
NCBI Accession NP_619527
UniProt O43521
Pathways PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BIM Antibody Target Details BIM Handling References for anti-BIM Antibody (ABIN361752) Images back to top
Application Notes
  • WB (1:400)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

2.5 μg/ml of SPC-164 was sufficient for detection of Bim in 20 μg of K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-BIM Antibody Target Details BIM Application Details References for anti-BIM Antibody (ABIN361752) Images back to top
Concentration 1 mg/mL
Buffer PBS, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-BIM Antibody (ABIN361752)

Product Details anti-BIM Antibody Target Details BIM Application Details Handling Images back to top
Background publications

Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998

OConnor, Strasser, OReilly, Hausmann, Adams, Cory, Huang: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998

Images

Product Details anti-BIM Antibody Target Details BIM Application Details Handling References for anti-BIM Antibody (ABIN361752) back to top
Supplier Images
 image for anti-BIM antibody (BCL2-Like 11 (Apoptosis Facilitator)) (ABIN361752) Bim BOD (IN2), K562 Cells.
Western Blotting (WB) image for anti-BIM antibody (BCL2-Like 11 (Apoptosis Facilitator)) (ABIN361752) Bim Bod A549 Cell lysate 10ug WB 1 in 1000.