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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||217 Antibodies||89 Antibodies||75 Antibodies|
|Immunostaining (ISt)||2 Antibodies|
|Immunoprecipitation (IP)||11 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||26 Antibodies||15 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||126 Antibodies||61 Antibodies||53 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies||26 Antibodies||26 Antibodies|
|Immunofluorescence (IF)||22 Antibodies||17 Antibodies||9 Antibodies|
|Antigen||BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BIM antibody is un-conjugated Alternatives|
Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-BIM AntibodyTarget Details BIM Application Details Handling References for anti-BIM Antibody (ABIN222941) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412620|
|Specificity||The antibody detects a 20 kDa Bim/BOD in samples from human, mouse and rat origins.|
|Immunogen||Synthetic peptide surrounding amino acid 25 of human Bim|
Target Details BIMProduct Details anti-BIM Antibody Application Details Handling References for anti-BIM Antibody (ABIN222941) Images back to top
|Alternative Name||Bim/Bod (BCL2L11 Antibody Abstract)|
Members of the Bcl-2 family proteins all contain Bcl-2 homology domains including BH1, BH2, BH3, and BH4. The BH3 domain is known to be important for the cell killing activity of Bcl-2 members including Bad, Bax, Bid, Bik, and the recently identified BH3 domain containing protein designated Bim/BOD. Bim/BOD interacts with diverse members of the Bcl-2 sub-family including Bcl-2, Bcl-x, and Bcl-w, to induce apoptosis. There are three splice variants: BimL, BimS, and BimEL, all of which share homology in their C-terminal BH3 domains. Of the isoforms, BimS is the most potent inducer of apoptosis. The mRNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
Synonyms: Bcl-2-like protein 11, BCL2L11
|Pathways||PI3K-Akt Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-BIM Antibody Target Details BIM Handling References for anti-BIM Antibody (ABIN222941) Images back to top
Western blot analysis (0.5-4 μg/mL).
Jurkat cell lysate can be used as a positive control.
However, the optimal conditions should be determined individually.
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-BIM Antibody Target Details BIM Application Details References for anti-BIM Antibody (ABIN222941) Images back to top
|Buffer||100 μg (0.5 mg/mL) affinity purified rabbit anti-Bim/BOD polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-BIM Antibody (ABIN222941)Product Details anti-BIM Antibody Target Details BIM Application Details Handling Images back to top
|Product cited in:||
Loh, Liou, Jiau et al.: "Hepatic effects among workers exposed to ethylene glycol monoethyl ether acetate." in: Industrial health, Vol. 46, Issue 5, pp. 463-9, 2008 (PubMed).
Chen, Huang, Wang: "Deficiency of Bim in dendritic cells contributes to overactivation of lymphocytes and autoimmunity." in: Blood, Vol. 109, Issue 10, pp. 4360-7, 2007 (PubMed).
Liou, Zhou, Pei et al.: "BimEL up-regulation potentiates AIF translocation and cell death in response to MPTP." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 10, pp. 1350-2, 2005 (PubMed).
ImagesProduct Details anti-BIM Antibody Target Details BIM Application Details Handling References for anti-BIM Antibody (ABIN222941) back to top