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anti-Human CDK2 antibody for Enzyme Immunoassay

Recommended CDK2 Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase 2 (CDK2) Antibodies
  • MGC84068
  • cinp
  • CINP
  • p33(CDK2)
  • A630093N05Rik
  • wu:fa10c02
  • zgc:56598
  • zgc:77684
  • eg1
  • CDKN2
  • cdk2L
  • MGC84068 protein
  • cyclin-dependent kinase 2 interacting protein
  • Cyclin-dependent kinase 2-interacting protein
  • cyclin dependent kinase 2
  • cyclin-dependent kinase 2
  • cyclin-dependent kinase 2 (CDK2)
  • cyclin-dependent kinase-2 alpha
  • MGC84068
  • CINP
  • cinp
  • Cdk2
  • CDK2
  • cdk2
  • LOC100154715
Epitope
pThr160
58
57
30
15
14
12
10
9
8
8
8
7
6
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
401
248
174
62
19
18
15
15
14
13
11
11
8
5
4
4
3
2
Host
Rabbit
321
136
1
Clonality
Polyclonal
Conjugate
This CDK2 antibody is un-conjugated
24
17
15
11
8
8
6
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
388
194
111
79
65
54
53
39
18
18
11
7
3
3
2
2
2
1
1
Supplier
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Catalog No. ABIN358106
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN359658 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 2
1 ABIN358539 EIA Rabbit Ig pThr14 Log in to see Polyclonal 4
1 ABIN1106645 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 1A6 1
1 ABIN116515 EIA IHC (fro) IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 3
1 ABIN400761 EIA IHC (p) IP WB Mouse IgG Log in to see 4i33
1 ABIN318814 EIA IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN303077 EIA IF IHC (p) IP WB Rabbit Log in to see Polyclonal

Top referenced anti-CDK2 antibody for Enzyme Immunoassay

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Application / Reactivity Human
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BioImaging (BI) 2 Antibodies
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Dot Blot (DB) 18 Antibodies
ELISA 166 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 53 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 64 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 98 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 79 Antibodies
Immunoprecipitation (IP) 49 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies

General

Antigen Cyclin-Dependent Kinase 2 (CDK2) Antibodies
Epitope pThr160
(58), (57), (30), (15), (14), (12), (10), (9), (8), (8), (8), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(401), (248), (174), (62), (19), (18), (15), (15), (14), (13), (11), (11), (8), (5), (4), (4), (3), (2)
Host Rabbit
(321), (136), (1)
Clonality
Polyclonal
Conjugate This CDK2 antibody is un-conjugated
(24), (17), (15), (11), (8), (8), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(388), (194), (111), (79), (65), (54), (53), (39), (18), (18), (11), (7), (3), (3), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-CDK2 Antibody

Target Details CDK2 Application Details Handling References for anti-CDK2 Antibody (ABIN358106) Images
Specificity This antibody detects CDK2 pThr160. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T160 of human Cdk2.
Isotype Ig

Target Details CDK2

Product Details anti-CDK2 Antibody Application Details Handling References for anti-CDK2 Antibody (ABIN358106) Images back to top
Antigen
Alternative Name CDK2 (CDK2 Antibody Abstract)
Background Cdk2 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.Synonyms: Cell division protein kinase 2, Cyclin dependent kinase 2, p33 protein kinase
Molecular Weight 39038 Da
Gene ID 1017, 9606
UniProt P24941
Research Area Cell Cycle, CD Antigens, Surface Receptors of Immune Cells
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA

Application Details

Product Details anti-CDK2 Antibody Target Details CDK2 Handling References for anti-CDK2 Antibody (ABIN358106) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CDK2 Antibody Target Details CDK2 Application Details References for anti-CDK2 Antibody (ABIN358106) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CDK2 Antibody (ABIN358106)

Product Details anti-CDK2 Antibody Target Details CDK2 Application Details Handling Images back to top
Background publications

Bicaku, Patel, Acevedo-Duncan: "Cyclin-dependent kinase activating kinase/Cdk7 co-localizes with PKC-iota in human glioma cells." in: Tissue & cell, Vol. 37, Issue 1, pp. 53-8, 2005

Lolli, Lowe, Brown, Johnson: "The crystal structure of human CDK7 and its protein recognition properties." in: Structure (London, England : 1993), Vol. 12, Issue 11, pp. 2067-79, 2004

Ito, Takeyama, Yamamoto, Sawatsubashi, Shirode, Kouzmenko, Tabata, Kato: "In vivo potentiation of human oestrogen receptor alpha by Cdk7-mediated phosphorylation." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 9, Issue 10, pp. 983-92, 2004

Zhou, Deng, Kashanchi, Brady, Shatkin, Kumar: "The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 22, pp. 12666-71, 2003

Kino, Tsukamoto, Chrousos: "Transcription factor TFIIH components enhance the GR coactivator activity but not the cell cycle-arresting activity of the human immunodeficiency virus type-1 protein Vpr." in: Biochemical and biophysical research communications, Vol. 298, Issue 1, pp. 17-23, 2002

Images

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Supplier Images
 image for anti-CDK2 antibody (Cyclin-Dependent Kinase 2) (pThr160) (ABIN358106) anti-Cyclin-Dependent Kinase 2 (CDK2) (pThr160) antibody