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anti-Human CDK2 antibody for Flow Cytometry

Recommended CDK2 Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase 2 (CDK2) Antibodies
  • A630093N05Rik
  • cdk2L
  • CDKN2
  • CINP
  • cinp
  • eg1
  • MGC84068
  • p33(CDK2)
  • wu:fa10c02
  • zgc:56598
  • zgc:77684
Epitope
AA 230-260, C-Term
53
47
25
13
9
7
7
7
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
298
171
125
41
9
9
9
7
7
6
5
4
4
Host
Rabbit
242
101
1
Clonality
Polyclonal
Conjugate
This CDK2 antibody is un-conjugated
14
11
10
7
6
6
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
281
142
75
52
42
34
33
31
14
11
9
8
3
2
1
1
1
1
Supplier
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Catalog No. ABIN391746
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN1106645 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 1A6 1
0.30226976 ABIN969506 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 1A6 1
0.30226976 ABIN2454506 FACS IF WB Mouse IgG2a Log in to see 1C4
0.30226976 ABIN2454523 FACS IF WB Mouse IgG2a Log in to see 1H5
0.30226976 ABIN2454567 FACS IF WB Mouse IgG2a Log in to see 4D3
0.30226976 ABIN2454507 FACS IF WB Mouse IgG1 Log in to see 2F10
0.30226976 ABIN2454524 FACS IF WB Mouse IgG2b Log in to see 4B4
0.30226976 ABIN2454601 FACS IF WB Mouse IgG2b Log in to see 6H2
0.30226976 ABIN2454606 FACS IF WB Mouse IgG2a Log in to see 1A8
0.30226976 ABIN2159985 FACS IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.30226976 ABIN1845617 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010482287 ABIN2454581 FACS IF IHC WB Mouse IgG2a Log in to see 2A5
0.0010482287 ABIN2454600 FACS IF IHC WB Mouse IgG2a Log in to see 2D9
0.0010482287 ABIN2230685 FACS IHC ELISA WB Rabbit IgG C-Term, AA 238-267 Log in to see Polyclonal
0.0010482287 ABIN2230689 FACS IHC ELISA WB FITC Rabbit IgG C-Term, AA 238-267 Log in to see Polyclonal
0.0010482287 ABIN2230687 FACS IHC ELISA WB APC Rabbit IgG C-Term, AA 238-267 Log in to see Polyclonal
0.0010482287 ABIN2230691 FACS IHC ELISA WB PE Rabbit IgG C-Term, AA 238-267 Log in to see Polyclonal
0.0010482287 ABIN2230677 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B157
0.0010482287 ABIN2137310 FACS IF WB Mouse IgG2a Log in to see 1C4
0.0010482287 ABIN2137312 FACS IF WB Mouse IgG2b Log in to see 4B4

Top referenced anti-CDK2 antibody for Flow Cytometry

  • Human Monoclonal CDK2 Primary Antibody for ICC, FACS - ABIN969506 (1 Publications): Markowski, Tyszkiewicz, Jarzab, Oczko-Wojciechowska, Gierek, Witkowska, Paluch, Kowalska, Wygoda, Lange, Jarzab: Metal-proteinase ADAM12, kinesin 14 and checkpoint suppressor 1 as new molecular markers of laryngeal carcinoma. in European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 2009 (PubMed)

Similar anti-CDK2 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
BioImaging (BI) 2 Antibodies
Dot Blot (DB) 11 Antibodies
ELISA 126 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 32 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 42 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 73 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 34 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies

General

Antigen Cyclin-Dependent Kinase 2 (CDK2) Antibodies
Epitope AA 230-260, C-Term
(53), (47), (25), (13), (9), (7), (7), (7), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(298), (171), (125), (41), (9), (9), (9), (7), (7), (6), (5), (4), (4)
Host Rabbit
(242), (101), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CDK2 antibody is un-conjugated
(14), (11), (10), (7), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(281), (142), (75), (52), (42), (34), (33), (31), (14), (11), (9), (8), (3), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CDK2 Antibody

Target Details CDK2 Application Details Handling References for anti-CDK2 Antibody (ABIN391746) Images
Specificity This CDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238~267 amino acids from the C-terminal region of human CDK2.
Predicted Reactivity Cow (Bovine),Hamster,Mouse (Murine),Rat (Rattus),Xenopus laevis
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This CDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 AA from the C-terminal region of human CDK2.
Clone RB3128
Isotype Ig

Target Details CDK2

Product Details anti-CDK2 Antibody Application Details Handling References for anti-CDK2 Antibody (ABIN391746) Images back to top
Antigen
Alternative Name CDK2 (CDK2 Antibody Abstract)
Background CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.
Synonyms: Cell division protein kinase 2, p33 protein kinase
Molecular Weight 33929 DA
Gene ID 1017
UniProt P24941
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure
Pathways PI3K-Akt Signaling, Cell Division Cycle

Application Details

Product Details anti-CDK2 Antibody Target Details CDK2 Handling References for anti-CDK2 Antibody (ABIN391746) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CDK2 Antibody Target Details CDK2 Application Details References for anti-CDK2 Antibody (ABIN391746) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CDK2 Antibody (ABIN391746)

Product Details anti-CDK2 Antibody Target Details CDK2 Application Details Handling Images back to top
Background publications

Moshinsky, Bellamacina, Boisvert, Huang, Hui, Jancarik, Kim, Rice: "SU9516: biochemical analysis of cdk inhibition and crystal structure in complex with cdk2." in: Biochemical and biophysical research communications, Vol. 310, Issue 3, pp. 1026-31, 2003

Chow, Siu, Ho, Ma, Ho, Poon: "Differential contribution of inhibitory phosphorylation of CDC2 and CDK2 for unperturbed cell cycle control and DNA integrity checkpoints." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 40815-28, 2003

ONions, Allday: "Epstein-Barr virus can inhibit genotoxin-induced G1 arrest downstream of p53 by preventing the inactivation of CDK2." in: Oncogene, Vol. 22, Issue 46, pp. 7181-91, 2003

Yun, Chae, Choi, Kim, Bang, Chung, Choi, Mantovani, Shin: "Cdk2-dependent phosphorylation of the NF-Y transcription factor and its involvement in the p53-p21 signaling pathway." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36966-72, 2003

Izumiya, Lin, Ellison, Levy, Mayeur, Izumiya, Kung: "Cell cycle regulation by Kaposi's sarcoma-associated herpesvirus K-bZIP: direct interaction with cyclin-CDK2 and induction of G1 growth arrest." in: Journal of virology, Vol. 77, Issue 17, pp. 9652-61, 2003

Images

Product Details anti-CDK2 Antibody Target Details CDK2 Application Details Handling References for anti-CDK2 Antibody (ABIN391746) back to top
Supplier Images
Western Blotting (WB) image for anti-CDK2 antibody (Cyclin-Dependent Kinase 2) (AA 230-260) (ABIN391746) Western blot analysis of anti-CDK2 Antibody (C-term) (ABIN391746) in Jurkat cell line...
Immunohistochemistry (IHC) image for anti-CDK2 antibody (Cyclin-Dependent Kinase 2) (AA 230-260) (ABIN391746) Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK2 Antibody ...
Flow Cytometry (FACS) image for anti-CDK2 antibody (Cyclin-Dependent Kinase 2) (AA 230-260) (ABIN391746) Flow cytometric analysis of jurkat cells using CDK2 Antibody (C-term)(bottom histogra...