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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|BioImaging (BI)||2 Antibodies||2 Antibodies|
|ELISA||30 Antibodies||43 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||7 Antibodies|
|Immunofluorescence (IF)||13 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies|
|Immunohistochemistry (IHC)||33 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||22 Antibodies|
|Immunoprecipitation (IP)||11 Antibodies|
|Western Blotting (WB)||80 Antibodies|
|Biochemical Assay (BCA)||1 Antibodies|
|Antigen||Cyclin-Dependent Kinase 2 (CDK2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CDK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-CDK2 AntibodyTarget Details CDK2 Application Details Handling References for anti-CDK2 Antibody (ABIN116515) Images
|Specificity||This antibody was prepared from monospecific antiserum by Delipidation and Defibrination. Antiserum will specifically react with a 33 kDa cdk2 protein. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk2 from other species may also occur.|
|Immunogen||CDK2 peptide corresponding to the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).|
Target Details CDK2Product Details anti-CDK2 Antibody Application Details Handling References for anti-CDK2 Antibody (ABIN116515) Images back to top
|Alternative Name||CDK2 (CDK2 Antibody Abstract)|
|Background||Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.Synonyms: Cell division protein kinase 2, Cyclin dependent kinase 2, p33 protein kinase|
|Research Area||Cell Cycle, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||PI3K-Akt Signaling, Cell Division Cycle|
Application DetailsProduct Details anti-CDK2 Antibody Target Details CDK2 Handling References for anti-CDK2 Antibody (ABIN116515) Images back to top
ELISA: 1/10,00-1/5,000. Multiple isoforms as well as phosphorylated forms of Cdk-2 maybe detected by this antibody. Wester Blot: 1/500-1/1,000. Immunoprecipitation: 1/100. Immunohistochemistry on Paraffin Embedded Sections: 1/200-1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CDK2 Antibody Target Details CDK2 Application Details References for anti-CDK2 Antibody (ABIN116515) Images back to top
|Concentration||85 mg/mL (by Refractometry)|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-CDK2 Antibody (ABIN116515)Product Details anti-CDK2 Antibody Target Details CDK2 Application Details Handling Images back to top
Elledge, Spottswood: "A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1." in: The EMBO journal, Vol. 10, Issue 9, pp. 2653-9, 1991 (PubMed).
Giordano, Lee, Scheppler et al.: "Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A." in: Science (New York, N.Y.), Vol. 253, Issue 5025, pp. 1271-5, 1991 (PubMed).
Tsai, Harlow, Meyerson: "Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase." in: Nature, Vol. 353, Issue 6340, pp. 174-7, 1991 (PubMed).