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anti-Human CHUK antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • AI256658
  • CHUK
  • Chuk1
  • Fbx24
  • Fbxo24
  • IKBKA
  • ikk
  • IKK-alpha
  • IKK1
  • ikk1
  • Ikka
  • IKKA
  • ikka
  • ikkalpha
  • NFKBIKA
  • TCF16
  • zgc:56539
Reactivity
Human, Mouse (Murine), Rat (Rattus)
311
108
82
4
4
Host
Rabbit
265
51
10
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
14
10
10
8
8
8
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
276
138
90
40
39
36
30
22
10
6
4
2
2
1
1
Supplier
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Catalog No. ABIN498205
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34211048 ABIN498244 IHC (p) WB Rabbit Log in to see Polyclonal
0.34211048 ABIN364294 IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.34211048 ABIN214043 IHC (p) WB Rabbit IgG Thr23 Log in to see Polyclonal
0.34211048 ABIN498850 IHC (p) Rabbit pSer180, pSer181 Log in to see Polyclonal
0.34211048 ABIN498938 IHC (p) Rabbit Log in to see Polyclonal
0.34211048 ABIN498939 IHC (p) Rabbit Log in to see Polyclonal
0.34211048 ABIN498851 IHC (p) Rabbit pSer176 Log in to see Polyclonal
0.34211048 ABIN615031 EIA IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.34211048 ABIN218505 IF IHC (p) IP ELISA WB Rabbit C-Term Log in to see Polyclonal
0.00089724595 ABIN681508 IF (p) IHC (p) WB Rabbit IgG AA 555-585 Log in to see Polyclonal 2
0.00089724595 ABIN197306 IHC (p) WB Rabbit Thr23 Log in to see Polyclonal 2
0.00089724595 ABIN196836 IHC (p) WB Rabbit pThr23 Log in to see Polyclonal 2
0.00089724595 ABIN265477 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.00089724595 ABIN3043812 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal
0.00089724595 ABIN302923 IHC (p) WB Rabbit IgG pThr23 Log in to see Polyclonal
0.00089724595 ABIN2888444 IHC (p) ELISA WB Rabbit IgG pThr23 Log in to see Polyclonal
0.00089724595 ABIN189444 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y463
0.00089724595 ABIN959200 FACS IHC (p) IP WB Mouse IgG1, kappa Log in to see 14A231
0.00089724595 ABIN438473 IHC (p) IP IHC PLA WB Rabbit IgG Log in to see Polyclonal
0.00089724595 ABIN2619268 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal

Top referenced anti-CHUK antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-CHUK Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 265 Antibodies 78 Antibodies 54 Antibodies
Proximity Ligation Assay (PLA) 5 Antibodies
Immunoprecipitation (IP) 38 Antibodies 8 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies 26 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 80 Antibodies 59 Antibodies 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies 22 Antibodies
Immunofluorescence (IF) 41 Antibodies 6 Antibodies 4 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(311), (108), (82), (4), (4)
Host Rabbit
(265), (51), (10), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(14), (10), (10), (8), (8), (8), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(276), (138), (90), (40), (39), (36), (30), (22), (10), (6), (4), (2), (2), (1), (1)
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling Images
Specificity This antibody detects endogenous levels of IKKα/β protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling Images back to top
Antigen
Alternative Name IKKA (CHUK Antibody Abstract)
Background The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκBα and specifically phosphorylates IκBα on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFκB activation in response to proinflammatory cytokines. Phosphorylation of IκB by IKKα is stimulated by the NFκB inducing kinase (NIK), which itself is a central regulator for NFκB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16
Molecular Weight approx. 85 kDa
Gene ID 1147
NCBI Accession NP_001269
UniProt O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN498205) Immunohistochemistry analyzes of IKKα/β antibody in paraffin-embedded human br...
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN498205) Western blot(WB) analysis of IKKα/β antibody at 1/500 dilution