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anti-Human CHUK antibody for Immunoprecipitation

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • AI256658
  • CHUK
  • Chuk1
  • Fbx24
  • Fbxo24
  • IKBKA
  • ikk
  • IKK-alpha
  • IKK1
  • ikk1
  • Ikka
  • IKKA
  • ikka
  • ikkalpha
  • NFKBIKA
  • TCF16
  • zgc:56539
Reactivity
Human, Mouse (Murine), Primate
348
129
84
18
4
4
1
Host
Mouse
275
77
11
1
Clonality
Monoclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
10
9
8
8
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
310
153
97
67
56
50
39
27
24
9
5
4
2
2
1
1
Supplier
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Catalog No. ABIN252647
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000895667 ABIN967550 IP WB Mouse IgG2b, kappa Log in to see B78-1 5
0.000895667 ABIN222974 IP WB Rabbit IgG Log in to see Polyclonal 2
0.000895667 ABIN438473 IHC (p) IP IHC PLA WB Rabbit IgG AA 350-400 Log in to see Polyclonal
0.000895667 ABIN364294 IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.000895667 ABIN208066 IP WB Mouse IgG Log in to see 14A231
0.000895667 ABIN959200 FACS IHC (p) IP WB Mouse IgG1, kappa Log in to see 14A231
0.000895667 ABIN1871859 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal
0.000895667 ABIN333144 IP ELISA WB Mouse IgG1, kappa Log in to see
0.000895667 ABIN4324239 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231 43
0.000895667 ABIN220382 ICC IP ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.000895667 ABIN241696 IP WB Rabbit AA 557-745 Log in to see Polyclonal
0.000895667 ABIN116874 EIA IP WB Rabbit C-Term Log in to see Polyclonal
0.000895667 ABIN218505 IF IHC (p) IP ELISA WB Rabbit C-Term Log in to see Polyclonal
0.000895667 ABIN303080 EIA IF IHC (p) IP WB Rabbit Log in to see Polyclonal
0.000895667 ABIN2470216 IF IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.000895667 ABIN2467456 IP IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.000895667 ABIN3197521 IP WB Biotin Mouse IgG1 Log in to see
0.000895667 ABIN1977815 IF IP IHC (p) PLA WB Rabbit IgG AA 350-400 Log in to see Polyclonal
0.000895667 ABIN1977817 IP Rabbit IgG AA 550-600 Log in to see Polyclonal
0.000895667 ABIN3197522 IP WB Biotin Mouse IgG1 Log in to see

Top referenced anti-CHUK antibody for Immunoprecipitation

Similar anti-CHUK Antibodies

Application / Reactivity Primate Mouse (Murine)
Flow Cytometry (FACS) 19 Antibodies 19 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 3 Antibodies 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies 43 Antibodies
Immunoprecipitation (IP) 19 Antibodies 26 Antibodies
Simple Western (SimWes) 3 Antibodies
Western Blotting (WB) 19 Antibodies
ELISA 28 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 57 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human, Mouse (Murine), Primate
(348), (129), (84), (18), (4), (4), (1)
Host Mouse
(275), (77), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHUK antibody is un-conjugated
(15), (11), (10), (9), (8), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(310), (153), (97), (67), (56), (50), (39), (27), (24), (9), (5), (4), (2), (2), (1), (1)
Pubmed 20 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN252647) Images
Specificity An 85 kDa band should be observed.
Purification Protein G purified
Immunogen This antibody was raised against a His-tagged full-length human IKK alpha protein.
Clone 14A231
Isotype IgG1 kappa

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK Antibody (ABIN252647) Images back to top
Antigen
Alternative Name IKK alpha (CHUK Antibody Abstract)
Background Gene Symbol: CHUK
Gene ID 1147, 12675
UniProt O15111
Research Area Immunology, Innate Immunity, Atherosclerosis, Transcription Factors, Apoptosis/Necrosis
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK Antibody (ABIN252647) Images back to top
Application Notes Western Blot 1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry (Intracellular): 0.25-0.5 μg/10^6 cells, Immunocytochemistry/Immunofluorescence 1:10, Immunoprecipitation 1-2 μg/mL, Immunohistochemistry-Paraffin 5 μg/mL, Immunohistochemistry-Frozen, Flow (Intracellular)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK Antibody (ABIN252647) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS. Does not contain BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CHUK Antibody (ABIN252647)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Product cited in:

Vincendeau, Hadian, Messias, Brenke, Halander, Griesbach, Greczmiel, Bertossi, Stehle, Nagel, Demski, Velvarska, Niessing, Geerlof, Sattler, Krappmann: "Inhibition of Canonical NF-κB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction." in: Scientific reports, Vol. 6, pp. 18934, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Shintaku, Peterson, Talbert, Gu, Ladner, Williams, Mousavi, Wang, Sartorelli, Guttridge: "MyoD Regulates Skeletal Muscle Oxidative Metabolism Cooperatively with Alternative NF-κB." in: Cell reports, Vol. 17, Issue 2, pp. 514-526, 2016 Method employed by authors: Western Blotting (WB)

Alameda, Gaspar, Ramírez, Navarro, Page, Suárez-Cabrera, Fernández, Mérida, Paramio, García-Fernández, Fernández-Aceñero, Casanova: "Deciphering the role of nuclear and cytoplasmic IKKα in skin cancer." in: Oncotarget, Vol. 7, Issue 20, pp. 29531-47, 2016 Method employed by authors: Immunocytochemistry (ICC), Immunofluorescence (IF) (Sample species: Human).

Mihalas, Meffert: "IKK kinase assay for assessment of canonical NF-κB activation in neurons." in: Methods in molecular biology (Clifton, N.J.), Vol. 1280, pp. 61-74, 2015

Remouchamps, Dejardin: "Methods to assess the activation of the alternative (noncanonical) NF-κB pathway by non-death TNF receptors." in: Methods in molecular biology (Clifton, N.J.), Vol. 1280, pp. 103-19, 2015

Ren, Takahashi, Liu, Loughran, Sun, Wang, Cheng: "HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains." in: Oncogene, Vol. 34, Issue 3, pp. 334-45, 2015 (Sample species: Human). Further details: Western Blotting

Gray, McCorkell, Chunduru, McKinlay, May: "Negative feedback regulation of NF-κB-inducing kinase is proteasome-dependent but does not require cellular inhibitors of apoptosis." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 341-6, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Richardson, Hammond, Coulombe, Saloranta, Nousiainen, Salonen, Berry, Hanley, Headon, Karikoski, Dixon: "Periderm prevents pathological epithelial adhesions during embryogenesis." in: The Journal of clinical investigation, Vol. 124, Issue 9, pp. 3891-900, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Gray, Remouchamps, McCorkell, Solt, Dejardin, Orange, May: "Noncanonical NF-κB signaling is limited by classical NF-κB activity." in: Science signaling, Vol. 7, Issue 311, pp. ra13, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Pradhan, Pratheek, Garai, Kumar, Meena, Ghosh, Singh, Kumari, Chandrashekar, Goswami, Chattopadhyay, Kar, Maiti: "Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells." in: Cell biology international, Vol. 38, Issue 10, pp. 1118-31, 2014 (Sample species: Human). Further details: Flow Cytometry,Immunochromatography

Das, Manna, Sinha, Datta, Das, Chakraborty, Ghosh, Saha, Dey: "Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model." in: PLoS ONE, Vol. 9, Issue 5, pp. e97599, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Buhrmann, Busch, Shayan, Shakibaei: "Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells." in: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22048-62, 2014 (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Schwitalla, Fingerle, Cammareri, Nebelsiek, Göktuna, Ziegler, Canli, Heijmans, Huels, Moreaux, Rupec, Gerhard, Schmid, Barker, Clevers, Lang, Neumann, Kirchner, Taketo, van den Brink, Sansom, Arkan et al.: "Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties. ..." in: Cell, Vol. 152, Issue 1-2, pp. 25-38, 2013 Further details: Western Blotting

Mihalas, Araki, Huganir, Meffert: "Opposing action of nuclear factor ?B and Polo-like kinases determines a homeostatic end point for excitatory synaptic adaptation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 42, pp. 16490-501, 2013 Further details: Immunoprecipitation

Bimonte, Barbieri, Palma, Luciano, Rea, Arra: "Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer." in: BioMed research international, Vol. 2013, pp. 810423, 2013 (Sample species: Human). Further details: Western Blotting

Cui, Li, Zhu, Liu, Songyang, Wang, Wang: "NLRP4 negatively regulates type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4." in: Nature immunology, Vol. 13, Issue 4, pp. 387-95, 2012

Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012

Bakkar, Ladner, Canan, Liyanarachchi, Bal, Pant, Periasamy, Li, Janssen, Guttridge: "IKK? and alternative NF-?B regulate PGC-1? to promote oxidative muscle metabolism." in: The Journal of cell biology, Vol. 196, Issue 4, pp. 497-511, 2012

Phromnoi, Reuter, Sung, Prasad, Kannappan, Yadav, Chanmahasathien, Limtrakul, Aggarwal et al.: "A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IκB kinase activation and sensitizes tumor cells to apoptosis by ..." in: Molecular pharmacology, Vol. 79, Issue 2, pp. 279-89, 2011 (Sample species: Human). Further details: Western Blotting

Hao, Rizzo, Osipo, Pannuti, Wyatt, Cheung, Sonenshein, Osborne, Miele: "Notch-1 activates estrogen receptor-alpha-dependent transcription via IKKalpha in breast cancer cells." in: Oncogene, Vol. 29, Issue 2, pp. 201-13, 2010

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN252647) back to top
Supplier Images
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody
Simple Western (SimWes) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Simple Western: IKK alpha Antibody (14A231) [ABIN2526474] - Simple Western lane view ...
Flow Cytometry (FACS) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Flow Cytometry: IKK alpha Antibody (14A231) [ABIN2526474] - Analysis using Azide Free...
Flow Cytometry (FACS) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Flow Cytometry: IKK alpha Antibody (14A231) [ABIN2526474] - Intracellular staining of...
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Western Blot: IKK alpha Antibody (14A231) [ABIN2526474] - Total protein from various ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) [ABIN2526474] - Analysis u...
Immunofluorescence (IF) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) [ABIN2526474] - A...
Immunofluorescence (IF) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) [ABIN2526474] - A...