You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human CHUK antibody for Immunoprecipitation

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase
  • chuk
  • CHUK
  • Chuk
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
338
150
103
19
12
11
10
7
6
5
5
4
2
1
Host
Mouse
259
81
12
1
Clonality
Monoclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
8
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
305
148
88
69
58
46
42
28
26
18
9
5
3
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN252647
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967550 IP WB Mouse IgG2b, kappa Log in to see B78-1 5
1 ABIN222974 IP WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN4324239 CyTOF FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231 43
1 ABIN1871859 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN438473 IHC (p) IP IHC PLA WB Rabbit AA 350-400 Log in to see Polyclonal
1 ABIN959200 FACS IHC (p) IP WB Mouse IgG1, kappa Log in to see 14A231
1 ABIN208066 IP WB Mouse IgG Log in to see 14A231
1 ABIN364294 IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN333144 IP WB Mouse IgG1, kappa Log in to see
1 ABIN241696 IP WB Rabbit AA 557-745 Log in to see Polyclonal
1 ABIN4324231 ChIP FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231
1 ABIN116874 EIA IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN303080 EIA IF IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN2470216 IF IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2467456 IP IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN220382 ICC IP ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN3197521 IP WB Biotin Mouse IgG1 Log in to see
1 ABIN3197522 IP WB Biotin Mouse IgG1 Log in to see
1 ABIN4324247 FACS IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see 14A231
1 ABIN1733082 IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-CHUK antibody for Immunoprecipitation

Similar anti-CHUK Antibodies

Application / Reactivity Rat (Rattus) Primate
Chromatin Immunoprecipitation (ChIP) 2 Antibodies 2 Antibodies
ELISA 18 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 18 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 16 Antibodies
Immunofluorescence (IF) 7 Antibodies 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 69 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human, Mouse (Murine), Primate, Rat (Rattus)
(338), (150), (103), (19), (12), (11), (10), (7), (6), (5), (5), (4), (2), (1)
Host Mouse
(259), (81), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(305), (148), (88), (69), (58), (46), (42), (28), (26), (18), (9), (5), (3), (2), (2), (1), (1), (1), (1)
Pubmed 20 references available
Supplier Log in to see

Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN252647) Images
Specificity An 85 kDa band should be observed.
Purification Protein G purified
Immunogen This antibody was raised against a His-tagged full-length human IKK alpha protein.
Clone 14A231
Isotype IgG1 kappa

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK Antibody (ABIN252647) Images back to top
Antigen
Alternative Name IKK alpha (CHUK Antibody Abstract)
Background Gene Symbol: CHUK
Gene ID 1147
UniProt O15111
Research Area Immunology, Innate Immunity, Atherosclerosis, Transcription Factors, Apoptosis/Necrosis
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK Antibody (ABIN252647) Images back to top
Application Notes Western Blot 1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry (Intracellular): 0.25-0.5 μg/10^6 cells, Immunocytochemistry/Immunofluorescence 1:10, Immunoprecipitation 1-2 μg/mL, Immunohistochemistry-Paraffin 5 μg/mL, Immunohistochemistry-Frozen, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP)Use in Flow Intracellular reported in scientific literature (PMID 24804954) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25133425). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in chromatin immunoprecipitation reported in scientific literature (PMID 27705798).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK Antibody (ABIN252647) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS - BSA free
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CHUK Antibody (ABIN252647)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Product cited in:

Diamanti, Gupta, Bennecke, De Oliveira, Ramakrishnan, Braczynski, Richter, Beli, Hu, Saleh, Mittelbronn, Dikic, Greten: "IKKα controls ATG16L1 degradation to prevent ER stress during inflammation." in: The Journal of experimental medicine, Vol. 214, Issue 2, pp. 423-437, 2017 Method employed by authors: Western Blotting (WB)

Vincendeau, Hadian, Messias, Brenke, Halander, Griesbach, Greczmiel, Bertossi, Stehle, Nagel, Demski, Velvarska, Niessing, Geerlof, Sattler, Krappmann: "Inhibition of Canonical NF-κB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction." in: Scientific reports, Vol. 6, pp. 18934, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Shintaku, Peterson, Talbert, Gu, Ladner, Williams, Mousavi, Wang, Sartorelli, Guttridge: "MyoD Regulates Skeletal Muscle Oxidative Metabolism Cooperatively with Alternative NF-κB." in: Cell reports, Vol. 17, Issue 2, pp. 514-526, 2016 Method employed by authors: Western Blotting (WB)

Alameda, Gaspar, Ramírez, Navarro, Page, Suárez-Cabrera, Fernández, Mérida, Paramio, García-Fernández, Fernández-Aceñero, Casanova: "Deciphering the role of nuclear and cytoplasmic IKKα in skin cancer." in: Oncotarget, Vol. 7, Issue 20, pp. 29531-47, 2016 Method employed by authors: Immunocytochemistry (ICC), Immunofluorescence (IF) (Sample species: Human).

Alameda, Navarro, Ramírez, Page, Suárez-Cabrera, Moreno-Maldonado, Paramio, del Carmen Fariña, Del Río, Fernández-Aceñero, Bravo, de Los Llanos Casanova: "IKKα regulates the stratification and differentiation of the epidermis: implications for skin cancer development." in: Oncotarget, Vol. 7, Issue 47, pp. 76779-76792, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB) (Sample species: Human).

Mihalas, Meffert: "IKK kinase assay for assessment of canonical NF-κB activation in neurons." in: Methods in molecular biology (Clifton, N.J.), Vol. 1280, pp. 61-74, 2015

Remouchamps, Dejardin: "Methods to assess the activation of the alternative (noncanonical) NF-κB pathway by non-death TNF receptors." in: Methods in molecular biology (Clifton, N.J.), Vol. 1280, pp. 103-19, 2015

Ren, Takahashi, Liu, Loughran, Sun, Wang, Cheng: "HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains." in: Oncogene, Vol. 34, Issue 3, pp. 334-45, 2015 (Sample species: Human). Further details: Western Blotting

Gray, McCorkell, Chunduru, McKinlay, May: "Negative feedback regulation of NF-κB-inducing kinase is proteasome-dependent but does not require cellular inhibitors of apoptosis." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 341-6, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Richardson, Hammond, Coulombe, Saloranta, Nousiainen, Salonen, Berry, Hanley, Headon, Karikoski, Dixon: "Periderm prevents pathological epithelial adhesions during embryogenesis." in: The Journal of clinical investigation, Vol. 124, Issue 9, pp. 3891-900, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Gray, Remouchamps, McCorkell, Solt, Dejardin, Orange, May: "Noncanonical NF-κB signaling is limited by classical NF-κB activity." in: Science signaling, Vol. 7, Issue 311, pp. ra13, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Pradhan, Pratheek, Garai, Kumar, Meena, Ghosh, Singh, Kumari, Chandrashekar, Goswami, Chattopadhyay, Kar, Maiti: "Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells." in: Cell biology international, Vol. 38, Issue 10, pp. 1118-31, 2014 (Sample species: Human). Further details: Flow Cytometry,Immunochromatography

Das, Manna, Sinha, Datta, Das, Chakraborty, Ghosh, Saha, Dey: "Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model." in: PLoS ONE, Vol. 9, Issue 5, pp. e97599, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Buhrmann, Busch, Shayan, Shakibaei: "Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells." in: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22048-62, 2014 (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Schwitalla, Fingerle, Cammareri, Nebelsiek, Göktuna, Ziegler, Canli, Heijmans, Huels, Moreaux, Rupec, Gerhard, Schmid, Barker, Clevers, Lang, Neumann, Kirchner, Taketo, van den Brink, Sansom, Arkan et al.: "Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties. ..." in: Cell, Vol. 152, Issue 1-2, pp. 25-38, 2013 Further details: Western Blotting

Mihalas, Araki, Huganir, Meffert: "Opposing action of nuclear factor ?B and Polo-like kinases determines a homeostatic end point for excitatory synaptic adaptation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 42, pp. 16490-501, 2013 Further details: Immunoprecipitation

Bimonte, Barbieri, Palma, Luciano, Rea, Arra: "Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer." in: BioMed research international, Vol. 2013, pp. 810423, 2013 (Sample species: Human). Further details: Western Blotting

Cui, Li, Zhu, Liu, Songyang, Wang, Wang: "NLRP4 negatively regulates type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4." in: Nature immunology, Vol. 13, Issue 4, pp. 387-95, 2012

Delhase, Kim, Lee, Naiki-Ito, Chen, Ahn, Murata, Kim, Lautsch, Kobayashi, Shirai, Karin, Nakanishi: "TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. E177-86, 2012

Bakkar, Ladner, Canan, Liyanarachchi, Bal, Pant, Periasamy, Li, Janssen, Guttridge: "IKK? and alternative NF-?B regulate PGC-1? to promote oxidative muscle metabolism." in: The Journal of cell biology, Vol. 196, Issue 4, pp. 497-511, 2012

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN252647) back to top
Supplier Images
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody
Simple Western (SimWes) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Simple Western: IKK alpha Antibody (14A231) [ABIN2526474] - Simple Western lane view ...
Flow Cytometry (FACS) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Flow Cytometry: IKK alpha Antibody (14A231) [ABIN2526474] - Analysis using Azide Free...
Flow Cytometry (FACS) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Flow Cytometry: IKK alpha Antibody (14A231) [ABIN2526474] - Intracellular staining of...
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Western Blot: IKK alpha Antibody (14A231) [ABIN2526474] - Total protein from various ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) [ABIN2526474] - Analysis u...
Immunofluorescence (IF) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) [ABIN2526474] - A...
Immunofluorescence (IF) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN252647) Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) [ABIN2526474] - A...