You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human CHUK antibody for Immunoprecipitation

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • AI256658
  • CHUK
  • Chuk1
  • Fbx24
  • Fbxo24
  • IKBKA
  • ikk
  • IKK-alpha
  • IKK1
  • ikk1
  • Ikka
  • IKKA
  • ikka
  • ikkalpha
  • NFKBIKA
  • TCF16
  • zgc:56539
Reactivity
Human
339
129
84
19
4
4
Host
Mouse
271
72
11
1
Clonality
Monoclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
10
9
8
8
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
303
145
95
64
54
47
37
26
22
10
4
4
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN967550
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008941957 ABIN222974 IP WB Rabbit IgG Log in to see Polyclonal 2
0.0008941957 ABIN208066 IP WB Mouse IgG Log in to see 14A231
0.0008941957 ABIN189444 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y463
0.0008941957 ABIN364294 IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008941957 ABIN959200 FACS IHC (p) IP WB Mouse IgG1, kappa Log in to see 14A231
0.0008941957 ABIN438473 IHC (p) IP IHC PLA WB Rabbit IgG Log in to see Polyclonal
0.0008941957 ABIN1871859 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0008941957 ABIN333144 IP ELISA WB Mouse IgG1, kappa Log in to see
0.0008941957 ABIN615031 EIA IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008941957 ABIN241696 IP WB Rabbit AA 557-745 Log in to see Polyclonal
0.0008941957 ABIN220382 ICC IP ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.0008941957 ABIN116874 EIA IP WB Rabbit C-Term Log in to see Polyclonal
0.0008941957 ABIN303080 EIA IF IHC (p) IP WB Rabbit Log in to see Polyclonal
0.0008941957 ABIN218505 IF IHC (p) IP ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0008941957 ABIN2467456 IP IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008941957 ABIN2736945 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0008941957 ABIN2470216 IF IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0008941957 ABIN3197521 IP WB Biotin Mouse IgG1 Log in to see
0.0008941957 ABIN2405010 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0008941957 ABIN1977815 IF IP IHC (p) PLA WB Rabbit IgG AA 350-400 Log in to see Polyclonal

Top referenced anti-CHUK antibody for Immunoprecipitation

Similar anti-CHUK Antibodies

Application / Reactivity Human
Control Peptide (CP) 1 Antibodies
ELISA 131 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 27 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 38 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 85 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 66 Antibodies
Immunoprecipitation (IP) 57 Antibodies
Proximity Ligation Assay (PLA) 5 Antibodies
Western Blotting (WB) 292 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human
(339), (129), (84), (19), (4), (4)
Host Mouse
(271), (72), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHUK antibody is un-conjugated
(15), (11), (10), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(303), (145), (95), (64), (54), (47), (37), (26), (22), (10), (4), (4), (2), (2), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN967550) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human IKKalpha
Clone B78-1
Isotype IgG2b, kappa

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK Antibody (ABIN967550) Images back to top
Antigen
Alternative Name IKK alpha (CHUK Antibody Abstract)
Background The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, I-kappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, I-kappaB becomes degraded and NF-kappaB is released to the nucleus. The release of I-kappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2). IKKalpha and IKKbeta are serine/threonine kinases which share approximately 50% sequence identity. In addition to their kinase domains, these proteins each contain leucine zipper and helix-loop-helix regions which facilitate their interaction with each other as well as interaction(s) with other proteins within the IKK complex. Both IKKalpha and IKKbeta phosphorylate critical residues of I-kappaB, thus targeting it for subsequent degradation. IKKalpha and IKKbeta become activated themselves following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK) and MEKK1. Thus the IKK kinases play an important role in the activation of NF-kappaB. IKKalpha migrates at a molecular weight of 85 kD in SDS-PAGE. Clone B78-1 recognizes human IKKalpha. It does not cross-react with IKKbeta. Full length, recombinant human IKKalpha was used as immunogen. The reactivity of B78-1 was verified by immunoprecipitation and western blot analysis.
Molecular Weight 85 kDa
Research Area Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK Antibody (ABIN967550) Images back to top
Application Notes Applications include western blot analysis (1-2 µg/ml). Immunoprecipitation (1-2 µg of antibody per one million cells or 1-2 µg of antibody for 300 µg of total protein) was also reported for clone B78-1. The following human cell lines are suggested as positive controls: A-204 (Rhabdomyosarcoma, ATCC HTB-82), RD (Rhabdomyosarcoma, ATCC CCL-136), HeLa (cervical carcinoma, ATCC CCL-2), Daudi (Burkitt lymphoma, ATCC CCL-213) and 293 transformed embryonic kidney cells (ATCC CRL-1573).
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK Antibody (ABIN967550) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CHUK Antibody (ABIN967550)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Product cited in:

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998

Nakano, Shindo, Sakon, Nishinaka, Mihara, Yagita, Okumura: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997

Woronicz, Gao, Cao, Rothe, Goeddel: "IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 866-9, 1997

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN967550) back to top
Supplier Images
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN967550) Western blot analysis of IKKalpha. Lane 1, lysate from Daubi B lymphoma cellswas prob...
 image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN967550) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (Image 2)
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN967550) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (Image 3)