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anti-Mouse (Murine) CHUK antibody for Enzyme Immunoassay

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • AI256658
  • CHUK
  • Chuk1
  • Fbx24
  • Fbxo24
  • IKBKA
  • ikk
  • IKK-alpha
  • IKK1
  • ikk1
  • Ikka
  • IKKA
  • ikka
  • ikkalpha
  • NFKBIKA
  • TCF16
  • zgc:56539
Reactivity
Human, Mouse (Murine), Rat (Rattus)
311
108
82
4
4
Host
Rabbit
265
51
10
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
14
10
10
8
8
8
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
276
138
90
40
39
36
30
22
9
6
4
2
2
1
1
Supplier
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Available images

Catalog No. ABIN265477
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088824704 ABIN615031 EIA IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.00088824704 ABIN116874 EIA IP WB Rabbit C-Term Log in to see Polyclonal

Similar anti-CHUK Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 13 Antibodies 28 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 3 Antibodies
Immunofluorescence (IF) 4 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 37 Antibodies 59 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies 26 Antibodies
Immunoprecipitation (IP) 6 Antibodies 8 Antibodies
Western Blotting (WB) 54 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(311), (108), (82), (4), (4)
Host Rabbit
(265), (51), (10), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(14), (10), (10), (8), (8), (8), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(276), (138), (90), (40), (39), (36), (30), (22), (9), (6), (4), (2), (2), (1), (1)
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling Images
Specificity This antibody detects endogenous levels of IKK-α protein. (region surrounding Met17)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling Images back to top
Antigen
Alternative Name IKKA (CHUK Antibody Abstract)
Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16
Molecular Weight approx. 85 kDa
Gene ID 1147
NCBI Accession NP_001269
UniProt O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
Application Notes ELISA: 1: 10000approx. 1:20000. WB: 1:500approx. 1:1000. IHC: 1:50approx. 1:200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling back to top
Supplier Images
 image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN265477) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody
 image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN265477) anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibody (Image 2)