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anti-Rat (Rattus) CHUK antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • AI256658
  • CHUK
  • Chuk1
  • Fbx24
  • Fbxo24
  • IKBKA
  • ikk
  • IKK-alpha
  • IKK1
  • ikk1
  • Ikka
  • IKKA
  • ikka
  • ikkalpha
  • NFKBIKA
  • TCF16
  • zgc:56539
Epitope
AA 555-585
63
29
23
17
15
15
13
12
12
11
10
9
9
9
8
7
7
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
339
128
83
19
4
4
Host
Rabbit
270
73
11
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
10
9
8
8
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
303
145
95
63
55
47
37
26
21
10
4
4
2
2
1
1
Supplier
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Supplier Product No.
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Catalog No. ABIN681508
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088487647 ABIN902067 IF (p) Alexa Fluor 555 Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN902066 IF (p) Alexa Fluor 488 Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN902065 IF (p) Alexa Fluor 350 Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN902068 IF (p) Alexa Fluor 647 Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN681512 IF (p) Cy5 Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN681511 IF (p) Cy3 Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN681514 IF (p) Cy7 Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN681513 IF (p) Cy5.5 Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN681515 IF (p) FITC Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN879731 IF (p) Alexa Fluor 488 Rabbit IgG pTyr463 Log in to see Polyclonal
0.00088487647 ABIN879732 IF (p) Alexa Fluor 555 Rabbit IgG pTyr463 Log in to see Polyclonal
0.00088487647 ABIN879734 IF (p) Alexa Fluor 647 Rabbit IgG pTyr463 Log in to see Polyclonal
0.00088487647 ABIN879733 IF (p) Alexa Fluor 350 Rabbit IgG pTyr463 Log in to see Polyclonal
0.00088487647 ABIN743277 IF (p) Cy5 Rabbit IgG pTyr463 Log in to see Polyclonal
0.00088487647 ABIN743278 IF (p) Cy5.5 Rabbit IgG pTyr463 Log in to see Polyclonal
0.00088487647 ABIN743276 IF (p) Cy3 Rabbit IgG pTyr463 Log in to see Polyclonal
0.00088487647 ABIN743280 IF (p) FITC Rabbit IgG pTyr463 Log in to see Polyclonal
0.00088487647 ABIN743279 IF (p) Cy7 Rabbit IgG pTyr463 Log in to see Polyclonal
0.00088487647 ABIN2814705 IF (p) Alexa Fluor 594 Rabbit IgG AA 555-585 Log in to see Polyclonal
0.00088487647 ABIN743273 IF (p) IHC (p) WB Rabbit IgG pTyr463 Log in to see Polyclonal

Top referenced anti-CHUK antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-CHUK Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 13 Antibodies 28 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies 20 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 5 Antibodies
Immunofluorescence (IF) 4 Antibodies 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 38 Antibodies 60 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies 46 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 55 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope AA 555-585
(63), (29), (23), (17), (15), (15), (13), (12), (12), (11), (10), (9), (9), (9), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(339), (128), (83), (19), (4), (4)
Host Rabbit
(270), (73), (11), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(15), (11), (10), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(303), (145), (95), (63), (55), (47), (37), (26), (21), (10), (4), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN681508) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human IKK alpha
Isotype IgG

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK Antibody (ABIN681508) Images back to top
Antigen
Alternative Name Ikk alpha (CHUK Antibody Abstract)
Background

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.

Subcellular location: Cytoplasm, Nucleus


Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, Transcription factor 16, TCF-16, CHUK, IKKA, TCF16
Gene ID 1147
UniProt O15111
Research Area Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK Antibody (ABIN681508) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK Antibody (ABIN681508) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-CHUK Antibody (ABIN681508)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Product cited in:

Huang, Yang, Wang, Ma, Cui, Huang, Sheng, Weng, Xu: "Immunostimulatory activity of protein hydrolysate from oviductus ranae on macrophage in vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 180234, 2015

Luo, Yang, Tang, Yao, Kong, He, Zhou: "Kaempferol alleviates insulin resistance via hepatic IKK/NF-?B signal in type 2 diabetic rats." in: International immunopharmacology, Vol. 28, Issue 1, pp. 744-50, 2015

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK Antibody (ABIN681508) back to top
Supplier Images
Immunofluorescence (IF) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (AA 555-585) (ABIN681508) Formalin-fixed and paraffin-embedded mouse lung labeled with Rabbit Anti-IKK alpha Po...