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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies||1 Antibodies|
|Simple Western (SimWes)||3 Antibodies||3 Antibodies|
|Western Blotting (WB)||294 Antibodies||92 Antibodies||51 Antibodies|
|Proximity Ligation Assay (PLA)||5 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||42 Antibodies||11 Antibodies||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||64 Antibodies||41 Antibodies||21 Antibodies|
|Immunohistochemistry (IHC)||83 Antibodies||57 Antibodies||35 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||18 Antibodies||16 Antibodies|
|Antigen||conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CHUK antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CHUK AntibodyTarget Details CHUK Application Details Handling Images
|Specificity||This antibody detects endogenous levels of IKKα/β protein. (region surrounding Asp173)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: IKKB
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
Target Details CHUKProduct Details anti-CHUK Antibody Application Details Handling Images back to top
|Alternative Name||IKKA (CHUK Antibody Abstract)|
|Background||The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16|
|Molecular Weight||approx. 85 kDa|
|Pathways||PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-CHUK Antibody Target Details CHUK Application Details Handling back to top