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anti-Rat (Rattus) CHUK antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase
  • chuk
  • CHUK
  • Chuk
Reactivity
Human, Mouse (Murine), Rat (Rattus)
348
127
81
19
4
4
1
Host
Rabbit
272
79
12
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
10
9
8
8
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
309
154
97
65
63
52
42
26
22
18
9
5
3
2
2
1
1
1
Supplier
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Catalog No. ABIN498244
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.203815 ABIN498205 IHC (p) WB Rabbit Log in to see Polyclonal
11.203815 ABIN498850 IHC (p) Rabbit pSer180, pSer181 Log in to see Polyclonal
11.203815 ABIN498939 IHC (p) Rabbit Log in to see Polyclonal
11.203815 ABIN498938 IHC (p) Rabbit Log in to see Polyclonal
11.203815 ABIN498851 IHC (p) Rabbit pSer176 Log in to see Polyclonal
1 ABIN196836 IHC (p) WB Rabbit pThr23 Log in to see Polyclonal 2
1 ABIN681508 IF (p) IHC (p) WB Rabbit IgG AA 555-585 Log in to see Polyclonal 2
1 ABIN265477 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN302923 IHC (p) WB Rabbit IgG pThr23 Log in to see Polyclonal
1 ABIN3043812 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal
1 ABIN318047 IHC (p) WB Rabbit pThr23 Log in to see Polyclonal
1 ABIN681517 IHC (p) WB HRP Rabbit IgG AA 555-585 Log in to see Polyclonal
1 ABIN743282 IHC (p) WB HRP Rabbit IgG pTyr463 Log in to see Polyclonal
1 ABIN681510 IHC (p) WB Biotin Rabbit IgG AA 555-585 Log in to see Polyclonal
1 ABIN743275 IHC (p) WB Biotin Rabbit IgG pTyr463 Log in to see Polyclonal
1 ABIN1733083 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN4324230 IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN743273 IF (p) IHC (p) WB Rabbit IgG pTyr463 Log in to see Polyclonal
1 ABIN1733082 IHC (p) IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN3030568 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal

Top referenced anti-CHUK antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-CHUK Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Cytometry by Time of Flight (CyTOF) 1 Antibodies 1 Antibodies
Simple Western (SimWes) 3 Antibodies 3 Antibodies
Western Blotting (WB) 294 Antibodies 92 Antibodies 51 Antibodies
Proximity Ligation Assay (PLA) 5 Antibodies 1 Antibodies
Immunoprecipitation (IP) 42 Antibodies 11 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 64 Antibodies 41 Antibodies 21 Antibodies
Immunohistochemistry (IHC) 83 Antibodies 57 Antibodies 35 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies 16 Antibodies

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(348), (127), (81), (19), (4), (4), (1)
Host Rabbit
(272), (79), (12), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(15), (11), (10), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(309), (154), (97), (65), (63), (52), (42), (26), (22), (18), (9), (5), (3), (2), (2), (1), (1), (1)
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling Images
Specificity This antibody detects endogenous levels of IKKα/β protein. (region surrounding Asp173)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: IKKB
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling Images back to top
Antigen
Alternative Name IKKA (CHUK Antibody Abstract)
Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16
Molecular Weight approx. 85 kDa
Gene ID 1147
NCBI Accession NP_001269
UniProt O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling Images back to top
Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN498244) Immunohistochemistry (IHC) analyzes of IKKα/β antibody in paraffin-embedded hu...
Western Blotting (WB) image for anti-CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (ABIN498244) Western blot analysis of IKKα/β antibody in extracts from Jurkat cells.