anti-Rat (Rattus) CHUK antibody for Western Blotting

Recommended CHUK Antibody (supplied by: Log in to see )

Antigen
conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
  • ikk
  • ikk1
  • ikka
  • ikkalpha
  • CHUK
  • IKBKA
  • IKK-alpha
  • IKK1
  • IKKA
  • NFKBIKA
  • TCF16
  • AI256658
  • Chuk1
  • Fbx24
  • Fbxo24
  • Ikka
  • zgc:56539
  • conserved helix-loop-helix ubiquitous kinase
  • chuk
  • CHUK
  • Chuk
Epitope
AA 151-200, pSer176
51
32
17
17
15
15
11
10
10
9
9
9
8
8
7
7
7
7
6
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
353
153
104
20
12
11
10
7
6
5
5
4
2
1
Host
Rabbit
273
83
12
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
15
11
11
9
8
8
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
318
150
94
78
62
48
44
29
26
19
9
5
3
3
2
2
1
1
1
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Catalog No. ABIN1531285
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN252647 ChIP FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 kappa Log in to see 14A231 71
3.2182703 ABIN2707589 IHC WB Rabbit N-Term, pSer23 Log in to see Polyclonal
3.2182703 ABIN2707590 IHC WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN3043812 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal
3.2182703 ABIN1531873 IHC ELISA WB Rabbit IgG AA 15-64, pThr23 Log in to see Polyclonal
3.2182703 ABIN1532904 IHC ELISA WB Rabbit IgG AA 15-64 Log in to see Polyclonal
3.2182703 ABIN3185147 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal
3.2182703 ABIN3182039 ELISA IHC WB Rabbit IgG pThr23 Log in to see Polyclonal
3.2182703 ABIN1870076 IHC WB Rabbit IgG pThr23 Log in to see Polyclonal
3.2182703 ABIN498205 IHC (p) WB Rabbit Log in to see Polyclonal
3.2182703 ABIN498244 IHC (p) WB Rabbit Log in to see Polyclonal
3.2182703 ABIN4324230 IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.2182703 ABIN1843986 IHC WB Rabbit pThr23 Log in to see Polyclonal
3.2182703 ABIN1843992 IHC WB Rabbit pSer176, pSer177 Log in to see Polyclonal
3.2182703 ABIN3030568 IHC (p) WB Rabbit IgG AA 411-745 Log in to see Polyclonal
3.2182703 ABIN1843988 IHC WB Rabbit Log in to see Polyclonal
3.2182703 ABIN2424879 IHC WB Rabbit IgG pThr23 Log in to see Polyclonal
3.2182703 ABIN1847264 IHC ELISA WB Rabbit Internal Region, pSer176, pSer177 Log in to see Polyclonal
3.2182703 ABIN2426593 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN5545335 IHC WB Rabbit IgG Log in to see Polyclonal

General

Antigen conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Antibodies
Epitope AA 151-200, pSer176
(51), (32), (17), (17), (15), (15), (11), (10), (10), (9), (9), (9), (8), (8), (7), (7), (7), (7), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(353), (153), (104), (20), (12), (11), (10), (7), (6), (5), (5), (4), (2), (1)
Host Rabbit
(273), (83), (12), (1)
Clonality
Polyclonal
Conjugate This CHUK antibody is un-conjugated
(15), (11), (11), (9), (8), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(318), (150), (94), (78), (62), (48), (44), (29), (26), (19), (9), (5), (3), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CHUK Antibody

Target Details CHUK Application Details Handling References for anti-CHUK antibody (ABIN1531285) Images
Specificity

IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) Antibody detects endogenous levels of IKK-alpha only when phosphorylated at Ser177.

PhosphorylationH:S176/S177 M:S176/S177 R:S176/S177

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Ser177.
Isotype IgG

Target Details CHUK

Product Details anti-CHUK Antibody Application Details Handling References for anti-CHUK antibody (ABIN1531285) Images back to top
Antigen
Alternative Name IKK-alpha (CHUK Antibody Abstract)
Background

Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IkappaB kinase, IkBKA, IKK-A, IKK-alpha, IKK1, IKKA, Inhibitor of nuclear factor kappa-B kinase alpha subunit, kinase IKK-alpha, NFKBIKA, Nuclear factor NFkapp

NCBI Gene Symbol: CHUK

Molecular Weight 84 kDa
Gene ID 1147, 3551
OMIM 600664
UniProt O15111, O14920
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-CHUK Antibody Target Details CHUK Handling References for anti-CHUK antibody (ABIN1531285) Images back to top
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Comment

Unigene-Number: Hs.198998, Hs.656458 (NCBI Gene Symbol: CHUK)

Restrictions For Research Use only

Handling

Product Details anti-CHUK Antibody Target Details CHUK Application Details References for anti-CHUK antibody (ABIN1531285) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-CHUK antibody (ABIN1531285)

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling Images back to top
Background publications

Régnier, Song, Gao, Goeddel, Cao, Rothe: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997

Images

Product Details anti-CHUK Antibody Target Details CHUK Application Details Handling References for anti-CHUK antibody (ABIN1531285) back to top
Supplier Images
Western Blotting (WB) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 151-200), (pSer176) antibody (ABIN1531285) Western blot analysis of extracts from NIH-3T3 cells treated with TNF 20ng/ml 30', us...
Immunohistochemistry (IHC) image for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 151-200), (pSer176) antibody (ABIN1531285) Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha (Phos...