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anti-Human Cyclin A antibody for Western Blotting

Recommended Cyclin A Antibody (supplied by: Log in to see )

Antigen
Cyclin A2 (CCNA2) Antibodies
  • ccn1
  • ccna
  • ccna1
  • CCN1
  • CCNA
  • AA408589
  • Ccn-1
  • Ccn1
  • Ccna
  • CycA2
  • Cyca
  • cb671
  • chunp6924
  • wu:fi36f03
  • CYCA
  • Cyclin-A2
  • ccna2
  • Cyclin-A
  • cyclinA
  • cyclin A2
  • cyclin A
  • ccna2
  • CCNA2
  • LOC100352785
  • Ccna2
  • LOC397933
Reactivity
Human, Mouse (Murine), Rat (Rattus)
245
132
84
53
21
21
21
19
4
3
3
3
3
3
2
2
1
1
Host
Mouse
187
100
1
Clonality
Monoclonal
Conjugate
This Cyclin A antibody is conjugated to Biotin
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
235
90
76
75
56
54
53
43
40
5
4
4
4
3
2
2
1
1
Supplier
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Catalog No. ABIN2853583
$ 404.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0741878 ABIN359545 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 2
3.0741878 ABIN359544 EIA WB Rabbit Ig N-Term Log in to see Polyclonal 3
3.0741878 ABIN391455 IHC (p) WB Rabbit Ig AA 358-389, C-Term Log in to see Polyclonal 3
3.0741878 ABIN2792174 WB Rabbit C-Term Log in to see Polyclonal 1
3.0741878 ABIN567779 IP WB Mouse IgG2a Log in to see E67-1 2
3.0741878 ABIN968444 BI WB Mouse IgG1 AA 26-144 Log in to see 25-Cyclin A 5
3.0741878 ABIN968445 BI WB Mouse IgG1 AA 26-144 Log in to see 25-Cyclin A 5
3.0741878 ABIN441337 ICC IF IHC (p) IP IHC WB Rabbit IgG Center Log in to see Polyclonal 2
3.0741878 ABIN967424 FACS IP WB Mouse IgE Log in to see BF683 6
3.0741878 ABIN967425 FACS IP WB Mouse IgE Log in to see BF683 6
3.0741878 ABIN4301457 ChIP ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
3.0741878 ABIN391454 FACS IHC (p) WB Rabbit Ig AA 51-84, N-Term Log in to see Polyclonal
3.0741878 ABIN2855544 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.0741878 ABIN3184138 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0741878 ABIN2779289 IHC WB Rabbit C-Term Log in to see Polyclonal
3.0741878 ABIN1533247 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0741878 ABIN3043481 IHC (p) WB Rabbit IgG AA 10-168 Log in to see Polyclonal
3.0741878 ABIN3043070 ICC WB Mouse IgG2a Log in to see CY-A1
3.0741878 ABIN1871530 IF WB Rabbit IgG Log in to see Polyclonal
3.0741878 ABIN2855543 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal

Top referenced anti-Cyclin A antibody for Western Blotting

Similar anti-Cyclin A Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 4 Antibodies 3 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies 4 Antibodies
Western Blotting (WB) 193 Antibodies 119 Antibodies 52 Antibodies
Immunoprecipitation (IP) 52 Antibodies 37 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 90 Antibodies 39 Antibodies 19 Antibodies
Immunohistochemistry (IHC) 74 Antibodies 35 Antibodies 17 Antibodies

General

Antigen Cyclin A2 (CCNA2) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(245), (132), (84), (53), (21), (21), (21), (19), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(187), (100), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin A antibody is conjugated to Biotin
(8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (2), (2)
Application Immunoprecipitation (IP), Western Blotting (WB)
(235), (90), (76), (75), (56), (54), (53), (43), (40), (5), (4), (4), (4), (3), (2), (2), (1), (1)
Pubmed 6 references available
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Product Details anti-Cyclin A Antibody

Target Details Cyclin A Application Details Handling References for anti-Cyclin A Antibody (ABIN2853583) Images
Specificity This antibody reacts with Cyclin A (55 kDa).
Purification Protein-A Agarose Chromatography of hybridoma supernatant.
Immunogen Bovine recombinant Cyclin A.
Clone E67-1
Isotype IgG2a

Target Details Cyclin A

Product Details anti-Cyclin A Antibody Application Details Handling References for anti-Cyclin A Antibody (ABIN2853583) Images back to top
Antigen
Alternative Name Cyclin A2 (CCNA2 Antibody Abstract)
Background Cyclin A is a 60 kDa cyclin that binds both cdk2 and p34cdc2 (cdk1), giving two distinct cyclin A kinase activities, one appearing in G1/S phase, the other in G2/M. The level of cyclin A protein peaks in S-phase, and the activity of the cdk2-cyclin A complexes is essential for DNA replication and progression through S phase. Cyclin A is thus a marker for actively proliferating cells and for cells in S-phase. In normal cells, cdk2-cyclin A complexes associate with PCNA and p21CIPP1/WAF1. In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcrIPPtion factors DRT F1 and E2F, and retinoblastoma protein, p110.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2
Gene ID 890
NCBI Accession NP_001228
UniProt P20248
Research Area Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase

Application Details

Product Details anti-Cyclin A Antibody Target Details Cyclin A Handling References for anti-Cyclin A Antibody (ABIN2853583) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details References for anti-Cyclin A Antibody (ABIN2853583) Images back to top
Concentration 20 µg/mL
Buffer PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.

References for anti-Cyclin A Antibody (ABIN2853583)

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling Images back to top
Background publications

Di Bartolo, Cannon, Liu, Renne, Chadburn, Boshoff, Cesarman: "KSHV LANA inhibits TGF-beta signaling through epigenetic silencing of the TGF-beta type II receptor." in: Blood, Vol. 111, Issue 9, pp. 4731-40, 2008

Ciarallo, Subramaniam, Hung, Lee, Kotchetkov, Sandhu, Milic, Slingerland: "Altered p27(Kip1) phosphorylation, localization, and function in human epithelial cells resistant to transforming growth factor beta-mediated G(1) arrest." in: Molecular and cellular biology, Vol. 22, Issue 9, pp. 2993-3002, 2002

Sandhu, Donovan, Bhattacharya, Stampfer, Worland, Slingerland: "Reduction of Cdc25A contributes to cyclin E1-Cdk2 inhibition at senescence in human mammary epithelial cells." in: Oncogene, Vol. 19, Issue 47, pp. 5314-23, 2000

Sandhu, Peehl, Slingerland: "p16INK4A mediates cyclin dependent kinase 4 and 6 inhibition in senescent prostatic epithelial cells." in: Cancer research, Vol. 60, Issue 10, pp. 2616-22, 2000

Slingerland, Hengst, Pan, Alexander, Stampfer, Reed: "A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells." in: Molecular and cellular biology, Vol. 14, Issue 6, pp. 3683-94, 1994

Dveksler, Dieffenbach, Cardellichio, McCuaig, Pensiero, Jiang, Beauchemin, Holmes: "Several members of the mouse carcinoembryonic antigen-related glycoprotein family are functional receptors for the coronavirus mouse hepatitis virus-A59." in: Journal of virology, Vol. 67, Issue 1, pp. 1-8, 1993

Images

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling References for anti-Cyclin A Antibody (ABIN2853583) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin A antibody (Cyclin A2)  (Biotin) (ABIN2853583) anti-Cyclin A2 (CCNA2) antibody (Biotin)