anti-Human Cyclin A antibody for Western Blotting

Recommended Cyclin A Antibody (supplied by: Log in to see )

Antigen
Cyclin A2 (CCNA2) Antibodies
  • ccn1
  • ccna
  • ccna1
  • CCN1
  • CCNA
  • AA408589
  • Ccn-1
  • Ccn1
  • Ccna
  • CycA2
  • Cyca
  • cb671
  • chunp6924
  • wu:fi36f03
  • CYCA
  • Cyclin-A2
  • ccna2
  • Cyclin-A
  • cyclinA
  • cyclin A2
  • cyclin A
  • ccna2
  • CCNA2
  • LOC100352785
  • Ccna2
  • LOC397933
Epitope
N-Term
28
21
12
6
5
5
4
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human
251
136
85
55
21
21
21
19
5
5
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
191
103
1
Clonality
Polyclonal
Conjugate
This Cyclin A antibody is un-conjugated
9
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6
6
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
241
94
78
76
59
54
54
43
40
4
4
4
4
3
2
2
1
1
Supplier
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Catalog No. ABIN359544
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3471746 ABIN359545 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 2
3.3471746 ABIN2853583 IP WB Biotin Mouse IgG2a Log in to see E67-1 6
3.3471746 ABIN2792174 WB Rabbit C-Term Log in to see Polyclonal 1
3.3471746 ABIN391455 IHC (p) WB Rabbit Ig AA 358-389, C-Term Log in to see Polyclonal 3
3.3471746 ABIN567779 IP WB Mouse IgG2a Log in to see E67-1 2
3.3471746 ABIN441337 ICC IF IHC (p) IP IHC WB Rabbit IgG Center Log in to see Polyclonal 2
3.3471746 ABIN968445 BI WB Mouse IgG1 AA 26-144 Log in to see 25-Cyclin A 5
3.3471746 ABIN968444 BI WB Mouse IgG1 AA 26-144 Log in to see 25-Cyclin A 5
3.3471746 ABIN4301457 ChIP ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
3.3471746 ABIN3043481 IHC (p) WB Rabbit IgG AA 10-168 Log in to see Polyclonal
3.3471746 ABIN967425 FACS IP WB Mouse IgE Log in to see BF683 6
3.3471746 ABIN967424 FACS IP WB Mouse IgE Log in to see BF683 6
3.3471746 ABIN2779289 IHC WB Rabbit C-Term Log in to see Polyclonal
3.3471746 ABIN3043070 ICC WB Mouse IgG2a Log in to see CY-A1 5
3.3471746 ABIN391454 FACS IHC (p) WB Rabbit Ig AA 51-84, N-Term Log in to see Polyclonal
3.3471746 ABIN2855544 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.3471746 ABIN1533247 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.3471746 ABIN3184138 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3471746 ABIN1871530 IF WB Rabbit IgG Log in to see Polyclonal
3.3471746 ABIN3197616 IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-Cyclin A antibody for Western Blotting

Similar anti-Cyclin A Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
Chromatin Immunoprecipitation (ChIP) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 72 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 52 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 39 Antibodies
Immunofluorescence (IF) 49 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 76 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 43 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 94 Antibodies
Immunoprecipitation (IP) 54 Antibodies
Western Blotting (WB) 198 Antibodies

General

Antigen Cyclin A2 (CCNA2) Antibodies
Epitope N-Term
(28), (21), (12), (6), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(251), (136), (85), (55), (21), (21), (21), (19), (5), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(191), (103), (1)
Clonality
Polyclonal
Conjugate This Cyclin A antibody is un-conjugated
(9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(241), (94), (78), (76), (59), (54), (54), (43), (40), (4), (4), (4), (4), (3), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-Cyclin A Antibody

Target Details Cyclin A Application Details Handling ProductDetails: References for anti-Cyclin A antibody (ABIN359544) Images
Specificity This antibody reacts to Cyclin A (CCNA2).
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-term of human CCNA2.
Isotype Ig

Target Details Cyclin A

Product Details anti-Cyclin A Antibody Application Details Handling ProductDetails: References for anti-Cyclin A antibody (ABIN359544) Images back to top
Antigen
Alternative Name Cyclin A2 (CCNA2 Antibody Abstract)
Background CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2
Gene ID 890, 9606
UniProt P20248
Research Area Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA

Application Details

Product Details anti-Cyclin A Antibody Target Details Cyclin A Handling ProductDetails: References for anti-Cyclin A antibody (ABIN359544) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details ProductDetails: References for anti-Cyclin A antibody (ABIN359544) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-Cyclin A antibody (ABIN359544)

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling Images back to top
Background publications

Tsang, Wang, Chen, Sánchez, Dynlacht: "SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression." in: The Journal of cell biology, Vol. 178, Issue 4, pp. 621-33, 2007

Henglein, Chenivesse, Wang, Eick, Bréchot: "Structure and cell cycle-regulated transcription of the human cyclin A gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 12, pp. 5490-4, 1994

Wang, Chenivesse, Henglein, Bréchot: "Hepatitis B virus integration in a cyclin A gene in a hepatocellular carcinoma." in: Nature, Vol. 343, Issue 6258, pp. 555-7, 1990

Images

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling ProductDetails: References for anti-Cyclin A antibody (ABIN359544) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin A antibody (Cyclin A2) (N-Term) (ABIN359544) Western blot analysis of anti-CCNA2(N-term) Pab in HL60 cell line lysates (35ug/lane)...