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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Chromatin Immunoprecipitation (ChIP)||2 Antibodies||2 Antibodies|
|Dot Blot (DB)||2 Antibodies||2 Antibodies|
|ELISA||17 Antibodies||50 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies|
|Immunofluorescence (IF)||19 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||17 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||19 Antibodies|
|Immunoprecipitation (IP)||21 Antibodies|
|Western Blotting (WB)||53 Antibodies|
|Cytometry by Time of Flight (CyTOF)||4 Antibodies|
|Flow Cytometry (FACS)||43 Antibodies|
|Antigen||Cyclin A2 (CCNA2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cyclin A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Cyclin A AntibodyTarget Details Cyclin A Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Cyclin A2 protein (region surrounding Trp250).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details Cyclin AProduct Details anti-Cyclin A Antibody Application Details Handling Images back to top
|Alternative Name||Cyclin A2 (CCNA2 Antibody Abstract)|
|Background||The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2|
|Molecular Weight||approx. 52 kDa|
|Research Area||Cell Cycle|
|Pathways||PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase|
Application DetailsProduct Details anti-Cyclin A Antibody Target Details Cyclin A Handling Images back to top
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling back to top