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anti-Rat (Rattus) Cyclin A antibody for Enzyme Immunoassay

Recommended Cyclin A Antibody (supplied by: Log in to see )

Antigen
Cyclin A2 (CCNA2) Antibodies
  • AA408589
  • cb671
  • Ccn-1
  • ccn1
  • Ccn1
  • CCN1
  • ccna
  • Ccna
  • CCNA
  • ccna1
  • ccna2
  • chunp6924
  • Cyca
  • CYCA
  • CycA2
  • Cyclin-A
  • Cyclin-A2
  • cyclinA
  • wu:fi36f03
Reactivity
Human, Mouse (Murine), Rat (Rattus)
133
48
33
31
5
3
3
2
2
2
2
2
1
Host
Rabbit
98
59
1
Clonality
Polyclonal
Conjugate
This Cyclin A antibody is un-conjugated
2
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
128
53
45
42
30
28
19
12
4
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN265377
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008857296 ABIN121516 EIA IHC (p) WB Rabbit Log in to see Polyclonal 3

Top referenced anti-Cyclin A antibody for Enzyme Immunoassay

Similar anti-Cyclin A Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 11 Antibodies 12 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 11 Antibodies
Immunofluorescence (IF) 14 Antibodies 19 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 8 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 33 Antibodies
Flow Cytometry (FACS) 2 Antibodies

General

Antigen Cyclin A2 (CCNA2) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(133), (48), (33), (31), (5), (3), (3), (2), (2), (2), (2), (2), (1)
Host Rabbit
(98), (59), (1)
Clonality
Polyclonal
Conjugate This Cyclin A antibody is un-conjugated
(2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(128), (53), (45), (42), (30), (28), (19), (12), (4), (4), (4), (3), (2), (1), (1), (1)
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Product Details anti-Cyclin A Antibody

Target Details Cyclin A Application Details Handling Images
Specificity This antibody detects endogenous levels of Cyclin A2 protein (region surrounding Trp250).
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details Cyclin A

Product Details anti-Cyclin A Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cyclin A2 (CCNA2 Antibody Abstract)
Background The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.Synonyms: CCN1, CCNA, CCNA2, Cyclin A, Cyclin-A2
Molecular Weight approx. 52 kDa
Gene ID 890
NCBI Accession NP_001228
UniProt P20248
Research Area Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling

Application Details

Product Details anti-Cyclin A Antibody Target Details Cyclin A Handling Images back to top
Application Notes ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-Cyclin A Antibody Target Details Cyclin A Application Details Handling back to top
Supplier Images
 image for anti-Cyclin A antibody (Cyclin A2) (ABIN265377) anti-Cyclin A2 (CCNA2) antibody
 image for anti-Cyclin A antibody (Cyclin A2) (ABIN265377) anti-Cyclin A2 (CCNA2) antibody (Image 2)