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anti-Cow (Bovine) Cyclin D1 antibody for ELISA

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • AI327039
  • bcl-1
  • bcl1
  • BCL1
  • cb161
  • ccnd1
  • CCND1
  • ccnd1a
  • ccnd1b
  • ccndbp1
  • cD1
  • cycd1
  • CycD1
  • Cyl-1
  • d11s287e
  • D11S287E
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • prad1
  • PRAD1
  • u21b31
  • U21B31
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ZHM
Epitope
AA 72-272
74
25
19
14
13
12
10
10
9
9
8
6
6
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine)
364
131
121
16
13
12
11
9
8
4
3
2
2
2
1
1
1
Host
Rabbit
299
114
1
Clonality
Polyclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
21
16
6
6
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
322
162
98
66
64
55
41
29
29
12
8
7
6
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN1858282
$ 415.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012190494 ABIN2618269 ELISA WB Biotin Rabbit IgG AA 72-272 Log in to see Polyclonal
0.0012190494 ABIN2592474 ELISA Rabbit IgG AA 72-272 Log in to see Polyclonal

Similar anti-Cyclin D1 Antibodies

Application / Reactivity Cow (Bovine)
ELISA 3 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 11 Antibodies

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope AA 72-272
(74), (25), (19), (14), (13), (12), (10), (10), (9), (9), (8), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(364), (131), (121), (16), (13), (12), (11), (9), (8), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(299), (114), (1)
Clonality
Polyclonal
Conjugate This Cyclin D1 antibody is un-conjugated
(21), (16), (6), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(322), (162), (98), (66), (64), (55), (41), (29), (29), (12), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-KCEEEVFPL AMNYLDRFLS LEPVKKSRLQ LLGATCMFVA SKMKETIPLT AEKLCIYTDN SIRPDELLHM ELVLVNKLKW NLAAMTPHDF IEHFLSKMPV AEENKQIIRK HAQTFVALCA TDVKFISNPP SMVAAGSVAA AAQGLHLGSA NGFLSYHRLT RFLSKVIRCD PDCLRACQEQ IEALLESSLR QAQQQNLDPK AA
Specificity It has been selected for its ability to recognize CCND1 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen CCND1 (AA 72-272)
Isotype IgG

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling Images back to top
Antigen
Pathways PI3K-Akt Signaling, Cell Division Cycle

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant CCND1 (Lys72~Ala272) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin D1 antibody (CCND1) (AA 72-272) (ABIN1858282) anti-Cyclin D1 (CCND1) (AA 72-272) antibody