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anti-Fruit Fly (Drosophila melanogaster) Cyclin D1 antibody for Western Blotting

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • ccnd1b
  • prad1
  • bcl1
  • d11s287e
  • u21b31
  • ccndbp1
  • ccnd1
  • ZHM
  • CCND1
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • ccnd1a
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • cyclin D1
  • Cyclin-D1-binding protein 1 homolog
  • cyclin Dx
  • zgc:162082
  • G1/S-specific cyclin-D1
  • ccnd1-b
  • ccnd1
  • ccndbp1
  • ccndx
  • zgc:162082
  • CCND1
  • Ccnd1
  • ccnd1-a
Epitope
Middle Region
76
24
19
16
13
12
10
9
9
8
8
6
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Human
470
242
231
44
21
19
18
16
16
14
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
312
198
19
4
Clonality
Polyclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
23
20
12
10
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
430
205
126
124
111
109
103
79
50
14
13
12
8
8
7
6
4
3
2
2
1
1
1
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Catalog No. ABIN2779291
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2618274 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN927283 WB Rabbit Middle Region Log in to see Polyclonal

Top referenced anti-Cyclin D1 antibody for Western Blotting

Similar anti-Cyclin D1 Antibodies

Application / Reactivity Human Cow (Bovine) Dog (Canine) Fruit Fly (Drosophila melanogaster)
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies
Western Blotting (WB) 370 Antibodies 18 Antibodies 14 Antibodies 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Immunoprecipitation (IP) 4 Antibodies 100 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 123 Antibodies
Immunohistochemistry (IHC) 102 Antibodies 4 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 48 Antibodies

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope Middle Region
(76), (24), (19), (16), (13), (12), (10), (9), (9), (8), (8), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Human
(470), (242), (231), (44), (21), (19), (18), (16), (16), (14), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(312), (198), (19), (4)
Clonality
Polyclonal
Conjugate This Cyclin D1 antibody is un-conjugated
(23), (20), (12), (10), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(430), (205), (126), (124), (111), (109), (103), (79), (50), (14), (13), (12), (8), (8), (7), (6), (4), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN2779291) Images
Blocking Peptide Blocking peptide for this product available: ABIN974598
Sequence ACQEQIEALL ESSLRQAQQN MDPKAAEEEE EEEEEVDLAC TPTDVRDVDI
Predicted Reactivity Human : 100 %, Bovine : 90 %, Dog : 90 %, Horse : 90 %
Characteristics This is a rabbit polyclonal antibody against CCND1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CCND1

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 Antibody (ABIN2779291) Images back to top
Antigen
Alternative Name CCND1 (CCND1 Antibody Abstract)
Background CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
Molecular Weight 34 kDa
Gene ID 595
NCBI Accession NM_053056, NP_444284
UniProt P24385
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 Antibody (ABIN2779291) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 295 AA

Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 Antibody (ABIN2779291) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Cyclin D1 Antibody (ABIN2779291)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Background publications

Kibel, Jin, Klim, Luly, A Roehl, Wu, Suarez: "Association between polymorphisms in cell cycle genes and advanced prostate carcinoma." in: The Prostate, Vol. 68, Issue 11, pp. 1179-86, 2008

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN2779291) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin D1 antibody (CCND1) (Middle Region) (ABIN2779291) WB Suggested Anti-CCND1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-Cyclin D1 antibody (CCND1) (Middle Region) (ABIN2779291) Lanes: Lane 1:341 µg PKO lysate
Lane 2: 041 µg PKO + Doxor lysate
Primar...