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|Antigen||Cyclin D1 (CCND1) Antibodies|
|Epitope||Middle Region Alternatives|
|Conjugate||This Cyclin D1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-Cyclin D1 AntibodyTarget Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN452823) Images
|Specificity||This antibody detects CCND1 at Center.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human|
|Purification||Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS|
|Immunogen||KLH conjugated synthetic peptide selected from the Center region of human CCND1|
Target Details Cyclin D1Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 antibody (ABIN452823) Images back to top
|Alternative Name||Cyclin D1 (CCND1 Antibody Abstract)|
|Background||CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of the gene encoding this protein, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.Synonyms: BCL-1 oncogene, BCL1, CCND1, Cyclin-D1, G1/S-specific cyclin-D1, PRAD-1 oncogene, PRAD1|
|Molecular Weight||33729 Da|
|Research Area||Cancer, Cell Cycle|
|Pathways||PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling|
Application DetailsProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 antibody (ABIN452823) Images back to top
Western blot: 1: 50 - 1: 100. ELISA: 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 antibody (ABIN452823) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
References for anti-Cyclin D1 antibody (ABIN452823)Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
He, Council, Feng, Chignell: "UVA-induced cell cycle progression is mediated by a disintegrin and metalloprotease/epidermal growth factor receptor/AKT/Cyclin D1 pathways in keratinocytes." in: Cancer research, Vol. 68, Issue 10, pp. 3752-8, 2008
Marsit, Black, Posner, Kelsey: "A genotype-phenotype examination of cyclin D1 on risk and outcome of squamous cell carcinoma of the head and neck." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 8, pp. 2371-7, 2008
ImagesProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN452823) back to top