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anti-Human Cyclin D1 antibody for Enzyme Immunoassay

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • AI327039
  • bcl-1
  • bcl1
  • BCL1
  • cb161
  • ccnd1
  • CCND1
  • ccnd1a
  • ccnd1b
  • ccndbp1
  • cD1
  • cycd1
  • CycD1
  • Cyl-1
  • d11s287e
  • D11S287E
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • prad1
  • PRAD1
  • u21b31
  • U21B31
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ZHM
Epitope
Middle Region
79
25
19
14
13
12
10
9
9
9
8
6
6
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
447
182
173
46
16
13
13
11
9
8
4
3
2
2
2
1
1
1
1
Host
Rabbit
310
188
1
Clonality
Polyclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
22
19
9
9
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
400
191
140
108
107
104
103
73
71
12
8
6
6
3
3
2
2
2
1
1
1
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Catalog No. ABIN452823
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008854274 ABIN357821 EIA WB Rabbit Ig N-Term Log in to see Polyclonal 3
0.0008854274 ABIN614708 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 Log in to see CD1-1
0.0008854274 ABIN1106870 EIA WB Mouse IgG1 Log in to see 3D8 2
0.0008854274 ABIN119000 EIA FACS IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see CD1-1
0.0008854274 ABIN615167 EIA IHC (p) WB Rabbit Ser90 Log in to see Polyclonal
0.0008854274 ABIN121518 EIA WB Rabbit Log in to see Polyclonal 2

Top referenced anti-Cyclin D1 antibody for Enzyme Immunoassay

Similar anti-Cyclin D1 Antibodies

Application / Reactivity Human
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Dot Blot (DB) 3 Antibodies
ELISA 166 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 107 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 4 Antibodies
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Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
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Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 66 Antibodies

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope Middle Region
(79), (25), (19), (14), (13), (12), (10), (9), (9), (9), (8), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(447), (182), (173), (46), (16), (13), (13), (11), (9), (8), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(310), (188), (1)
Clonality
Polyclonal
Conjugate This Cyclin D1 antibody is un-conjugated
(22), (19), (9), (9), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(400), (191), (140), (108), (107), (104), (103), (73), (71), (12), (8), (6), (6), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN452823) Images
Specificity This antibody detects CCND1 at Center.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen KLH conjugated synthetic peptide selected from the Center region of human CCND1

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 Antibody (ABIN452823) Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of the gene encoding this protein, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.Synonyms: BCL-1 oncogene, BCL1, CCND1, Cyclin-D1, G1/S-specific cyclin-D1, PRAD-1 oncogene, PRAD1
Molecular Weight 33729 Da
Gene ID 595
NCBI Accession NP_444284
UniProt P24385
Research Area Cancer, Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 Antibody (ABIN452823) Images back to top
Application Notes Western blot: 1: 50 - 1: 100. ELISA: 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 Antibody (ABIN452823) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-Cyclin D1 Antibody (ABIN452823)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Background publications

He, Council, Feng, Chignell: "UVA-induced cell cycle progression is mediated by a disintegrin and metalloprotease/epidermal growth factor receptor/AKT/Cyclin D1 pathways in keratinocytes." in: Cancer research, Vol. 68, Issue 10, pp. 3752-8, 2008

Marsit, Black, Posner, Kelsey: "A genotype-phenotype examination of cyclin D1 on risk and outcome of squamous cell carcinoma of the head and neck." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 8, pp. 2371-7, 2008

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN452823) back to top
Supplier Images
 image for anti-Cyclin D1 antibody (CCND1) (Middle Region) (ABIN452823) anti-Cyclin D1 (CCND1) (Middle Region) antibody