anti-Monkey Cyclin D1 antibody for Immunocytochemistry

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • ccnd1b
  • prad1
  • bcl1
  • d11s287e
  • u21b31
  • ccndbp1
  • ccnd1
  • ZHM
  • CCND1
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • ccnd1a
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • cyclin D1
  • Cyclin-D1-binding protein 1 homolog
  • cyclin Dx
  • zgc:162082
  • G1/S-specific cyclin-D1
  • ccnd1-b
  • ccnd1
  • ccndbp1
  • ccndx
  • zgc:162082
  • CCND1
  • Ccnd1
  • ccnd1-a
Epitope
AA 285-295
79
39
25
18
16
14
12
11
10
9
8
8
6
6
6
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Bat, Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
490
254
244
44
20
20
19
16
16
14
5
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
344
204
19
5
Clonality
Polyclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
27
24
16
11
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
466
209
133
127
118
109
108
79
51
14
13
12
8
7
7
6
6
3
1
1
1
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Catalog No. ABIN211137
$ 903.83
Plus shipping costs $45.00

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope AA 285-295
(79), (39), (25), (18), (16), (14), (12), (11), (10), (9), (8), (8), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
(490), (254), (244), (44), (20), (20), (19), (16), (16), (14), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(344), (204), (19), (5)
Clonality
Polyclonal
Conjugate This Cyclin D1 antibody is un-conjugated
(27), (24), (16), (11), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(466), (209), (133), (127), (118), (109), (108), (79), (51), (14), (13), (12), (8), (7), (7), (6), (6), (3), (1), (1), (1)
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling Images
Specificity Recognizes p36 Cyclin D1 and cross-reacts with p34 Cyclin D2, which has a highly homologous epitope. No cross-reactivity with Cyclin A or Cyclin B. Shows reactivity with a 28kD band that can competed away by pre-incubating antibody with the peptide immunogen. Also detect a nonspecific band at 21kD. Species cross-reactivity: mouse and rat.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Bat (100%) Elephant, Panda, Bovine, Dog, Horse, Turkey, Chicken, Platypus, Lizard, Xenopus, Salmon, Pufferfish (91%) Opossum, Stickleback, Zebrafish (82%).
Purification Protein A purified
Immunogen Synthetic peptide corresponding to the 11 C-terminal amino acids (residues 285-295, sequence: CTPTDVRDVDI) of human Cyclin D1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Bat (100%), Elephant, Panda, Bovine, Dog, Horse, Turkey, Chicken, Platypus, Lizard, Xenopus, Salmon, Pufferfish (91%), Opossum, Stickleback, Zebrafish (82%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CCND1 / Cyclin D1 (CCND1 Antibody Abstract)
Background Name/Gene ID: CCND1

Synonyms: CCND1, B-cell lymphoma 1 protein, BCL-1 oncogene, B-cell CLL/lymphoma 1, BCL-1, Cyclin D1, G1/S-specific cyclin-D1, U21B31, PRAD1, BCL1, D11S287E, PRAD1 oncogene
Gene ID 595
UniProt P24385
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling Images back to top
Application Notes Approved: Func, ICC, IP, WB

Usage: Western Blot Analysis 2: At 0.5-2 μg/mL, this antibody detected Cyclin D in a RIPA lysates of human A431 cells, mouse 3T3 fibroblasts. Positive Antigen Control: EGF-stimulated A431 cell lysate. Use 20 μg per lane. Immunoprecipitation: 5 μg of antibody immunoprecipitated Cyclin D from a RIPA lysate of human A431 cells. An H1 kinase assay can be performed with the immunoprecipitate, as reported previously. Immunocytochemistry: At 10 μg/mL, a previous lot of antibody detected Cyclin D in cells fixed with 4 % paraformaldehyde/0.1 % Triton X-100. Western Blot Analysis EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Cyclin D (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.