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|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||2 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||67 Antibodies|
|Functional Studies (Func)||8 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||48 Antibodies|
|Immunofluorescence (IF)||54 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||13 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||55 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||4 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||37 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||60 Antibodies|
|Immunoprecipitation (IP)||74 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Western Blotting (WB)||187 Antibodies|
|Antigen||Cyclin D1 (CCND1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cyclin D1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Cyclin D1 AntibodyTarget Details Cyclin D1 Application Details Handling Images
|Purpose||Mouse IgG monoclonal antibody for Cyclin D1, cyclin D1 (CCND1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,mouse,rat. No cross reactivity with other proteins.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Mouse IgG monoclonal antibody for Cyclin D1, cyclin D1 (CCND1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,mouse,rat.
Gene Name: cyclin D1
Protein Name: G1/S-specific cyclin-D1
|Immunogen||Recombinant human cyclin D1 protein.|
Target Details Cyclin D1Product Details anti-Cyclin D1 Antibody Application Details Handling Images back to top
|Alternative Name||CCND1 (CCND1 Antibody Abstract)|
D-type cyclins(cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. CyclinD1(CCND1), also known as BCL1. Expression of cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. CCND1 gene to 11q13. cyclin D1 gene can function as an oncogene. Cyclin D1 expression is regulated by the retinoblastoma protein.
Synonyms: BCL1|U21B31|D11S287|PRAD1|B-CELL LEUKEMIA 1|B-cell CLL/lymphoma 1|G1/S-specific cyclin D1|parathyroid adenomatosis 1|B-cell lymphoma 1 protein|BCL-1 oncogene|PRAD1 oncogene
|Gene ID||595, 12443, 58919|
|UniProt||P24385, P25322, P39948|
|Pathways||PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling|
Application DetailsProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling Images back to top
WB: Concentration: 4 μg/mL, Species: Human, mouse, rat
IHC-P: Concentration: 8 μg/mL, Species: Human, mouse, rat
IHC-F: Concentration: 8 μg/mL, Species: Human, mouse, rat
ICC: Concentration: 1 μg/mL, Species: Human, mouse, rat
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Images back to top
|Reconstitution||Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.|
|Buffer||Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling back to top