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anti-Rat (Rattus) Cyclin D1 antibody for Immunocytochemistry

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • AI327039
  • bcl-1
  • bcl1
  • BCL1
  • cb161
  • ccnd1
  • CCND1
  • ccnd1a
  • ccnd1b
  • ccndbp1
  • cD1
  • cycd1
  • CycD1
  • Cyl-1
  • d11s287e
  • D11S287E
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • prad1
  • PRAD1
  • u21b31
  • U21B31
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ZHM
Reactivity
Human, Rat (Rattus)
449
183
174
46
16
13
13
11
9
8
4
3
2
2
2
1
1
1
1
Host
Mouse
312
188
1
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
22
19
9
9
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
402
191
141
108
107
106
103
72
71
12
8
7
6
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN302101
$ 288.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN3043639 ICC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see DCS-6
0.0009962841 ABIN256781 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term, AA 245-295 Log in to see EPR2241
0.0009962841 ABIN189278 ICC IF IHC (p) IP IHC WB Rabbit IgG N-Term, AA 1-50 Log in to see EP272Y
0.0009962841 ABIN2471067 ICC FACS IP IHC ELISA WB Mouse IgG1 Log in to see CD1-1
0.0009962841 ABIN1077957 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-295 Log in to see Polyclonal
0.0009962841 ABIN1723570 ICC IHC (fro) FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see CD1-1
0.0009962841 ABIN4301539 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301540 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301541 FACS ICC IF IHC (p) IP WB Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301563 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301564 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301565 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301566 FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301567 FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301568 FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301569 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301570 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301571 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301573 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG2a kappa Log in to see SPM587
0.0009962841 ABIN4301574 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a kappa Log in to see SPM587

Top referenced anti-Cyclin D1 antibody for Immunocytochemistry

Similar anti-Cyclin D1 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 65 Antibodies 166 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 57 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 62 Antibodies
Immunofluorescence (IF) 64 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 51 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 66 Antibodies
Immunoprecipitation (IP) 67 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 149 Antibodies
Blocking Reagent (BR) 2 Antibodies
Dot Blot (DB) 3 Antibodies

General

Antigen Cyclin D1 (CCND1) Antibodies
Reactivity Human, Rat (Rattus)
(449), (183), (174), (46), (16), (13), (13), (11), (9), (8), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(312), (188), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is un-conjugated
(22), (19), (9), (9), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(402), (191), (141), (108), (107), (106), (103), (72), (71), (12), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN302101) Images
Specificity The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Purified cyclin D1 protein
Clone CD1-1
Isotype IgG1

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 Antibody (ABIN302101) Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Research Area Cancer, Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 Antibody (ABIN302101) Images back to top
Application Notes Flow Cytometry:
Application note: Membrane permeabilization is required.
Immunoprecipitation: Recommended dilution: 1 µg/mL
Western Blotting: Recommended dilution: 1 µg/mL
Immunohistochemistry (paraffin sections)
Pretreatment: Heat treatment, sodium citrate buffer pH 6.0.
Immunohistochemistry (frozen sections) Recommended dilution: 2 µg/mL
Positive tissue: colon.
Immunocytochemistry: Recommended dilution: 1 µg/mL

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 Antibody (ABIN302101) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-Cyclin D1 Antibody (ABIN302101)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Background publications

Diehl, Cheng, Roussel, Sherr: "Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization." in: Genes & development, Vol. 12, Issue 22, pp. 3499-511, 1999

Diehl, Zindy, Sherr: "Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway." in: Genes & development, Vol. 11, Issue 8, pp. 957-72, 1997