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anti-Rat (Rattus) Cyclin D1 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • AI327039
  • bcl-1
  • bcl1
  • BCL1
  • cb161
  • ccnd1
  • CCND1
  • ccnd1a
  • ccnd1b
  • ccndbp1
  • cD1
  • cycd1
  • CycD1
  • Cyl-1
  • d11s287e
  • D11S287E
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • prad1
  • PRAD1
  • u21b31
  • U21B31
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • ZHM
Epitope
full length
79
25
19
14
13
12
10
10
9
9
8
6
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
447
181
172
46
16
13
13
11
9
8
4
3
2
2
2
1
1
1
1
Host
Mouse
311
187
1
Clonality
Monoclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
22
19
9
9
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
400
191
140
107
107
104
103
73
70
12
8
7
6
3
2
2
2
2
1
1
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Catalog No. ABIN967539
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Top referenced anti-Cyclin D1 antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-Cyclin D1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 64 Antibodies 64 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 56 Antibodies 52 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 62 Antibodies
Immunofluorescence (IF) 64 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 50 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 64 Antibodies
Immunoprecipitation (IP) 66 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 147 Antibodies

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope full length
(79), (25), (19), (14), (13), (12), (10), (10), (9), (9), (8), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(447), (181), (172), (46), (16), (13), (13), (11), (9), (8), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(311), (187), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cyclin D1 antibody is un-conjugated
(22), (19), (9), (9), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoprecipitation (IP), Western Blotting (WB)
(400), (191), (140), (107), (107), (104), (103), (73), (70), (12), (8), (7), (6), (3), (2), (2), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN967539) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Full-length Cyclin D1 Recombinant Protein
Clone DCS-6
Isotype IgG2a

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 Antibody (ABIN967539) Images back to top
Antigen
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Cyclins and cyclin-dependent kinases (Cdks) have been shown to be subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. Specific substrates for Cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. D-type cyclins are involved in regulating the passage of mammalian cells through G1. In SDS-PAGE, D-type cyclins migrate at the following molecular weights: cyclin D1 (36 kDa), cyclin D2 (35 kDa), and cyclin D3 [31 and 34 kDa (doublet)]. Rodent cyclin D1 homologues (Cyl1) have been reported to typically migrate as a 36 kDa doublet. The mouse anti-human cyclin D1 antibody (clone DCS-6) recognizes human cyclin D1 (36 kDa) and has been reported to crossreact with rat and mouse cyclin D1 homologues (Cyl1). It does not crossreact with human cyclins D2 and D3.
Molecular Weight 36 kDa
Research Area Cancer, Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 Antibody (ABIN967539) Images back to top
Comment

Related Products: ABIN968585, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 Antibody (ABIN967539) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Cyclin D1 Antibody (ABIN967539)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Product cited in:

Darzynkiewicz, Gong, Juan, Ardelt, Traganos: "Cytometry of cyclin proteins." in: Cytometry, Vol. 25, Issue 1, pp. 1-13, 1997

de Boer, Schuuring, Dreef, Peters, Bartek, Kluin, van Krieken: "Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma." in: Blood, Vol. 86, Issue 7, pp. 2715-23, 1995

Lukas, Pagano, Staskova, Draetta, Bartek: "Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines." in: Oncogene, Vol. 9, Issue 3, pp. 707-18, 1994

Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, 1994

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 Antibody (ABIN967539) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin D1 antibody (CCND1) (full length) (ABIN967539) Western blot analysis of Cyclin D1. MCF7 cell lysates (Human breast adenocarcinoma, A...
 image for anti-Cyclin D1 antibody (CCND1) (full length) (ABIN967539) anti-Cyclin D1 (CCND1) (full length) antibody (Image 2)
Western Blotting (WB) image for anti-Cyclin D1 antibody (CCND1) (full length) (ABIN967539) anti-Cyclin D1 (CCND1) (full length) antibody (Image 3)