anti-Rat (Rattus) Cyclin D1 antibody for Western Blotting

Recommended Cyclin D1 Antibody (supplied by: Log in to see )

Antigen
Cyclin D1 (CCND1) Antibodies
  • ccnd1b
  • prad1
  • bcl1
  • d11s287e
  • u21b31
  • ccndbp1
  • ccnd1
  • ZHM
  • CCND1
  • BCL1
  • D11S287E
  • PRAD1
  • U21B31
  • AI327039
  • CycD1
  • Cyl-1
  • bcl-1
  • cD1
  • ccnd1a
  • cb161
  • cycd1
  • etID37810.7
  • fb52e01
  • fc45c08
  • fc83a12
  • id:ibd1198
  • wu:fb52e01
  • wu:fc45c08
  • wu:fc83a12
  • cyclin D1
  • Cyclin-D1-binding protein 1 homolog
  • cyclin Dx
  • zgc:162082
  • G1/S-specific cyclin-D1
  • ccnd1-b
  • ccnd1
  • ccndbp1
  • ccndx
  • zgc:162082
  • CCND1
  • Ccnd1
  • ccnd1-a
Epitope
Middle Region
79
24
21
16
14
11
11
10
9
8
8
6
6
6
5
5
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Yeast, Zebrafish
488
244
236
44
21
19
19
16
15
14
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
325
204
19
4
Clonality
Polyclonal
Conjugate
This Cyclin D1 antibody is un-conjugated
26
23
15
10
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
447
209
133
127
117
109
109
80
51
14
13
12
8
8
7
6
5
3
1
1
1
Options
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Catalog No. ABIN2779291
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9061391 ABIN2792166 WB Rabbit Middle Region Log in to see Polyclonal 1
2.9061391 ABIN2778440 WB Rabbit C-Term Log in to see Polyclonal
2.9061391 ABIN1106872 IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see CY-D1 3
2.9061391 ABIN2705960 WB Rabbit Center Log in to see Polyclonal
2.9061391 ABIN967539 IHC (f) IHC (fro) ICS IP WB Mouse IgG2a full length Log in to see DCS-6 4
2.9061391 ABIN1532579 ELISA WB Rabbit IgG Log in to see Polyclonal 3
2.9061391 ABIN671016 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 70-120 Log in to see Polyclonal 15
2.9061391 ABIN115708 IHC (p) IP WB Rabbit IgG C-Term Log in to see SP4
2.9061391 ABIN3044355 WB Rabbit IgG AA 219-238, C-Term Log in to see Polyclonal 23
2.9061391 ABIN1531265 ELISA WB Rabbit IgG pThr286 Log in to see Polyclonal
2.9061391 ABIN1106869 WB Rabbit IgG N-Term Log in to see Polyclonal
2.9061391 ABIN3184144 ELISA IHC WB Rabbit IgG Ser166 Log in to see Polyclonal
2.9061391 ABIN3182196 ELISA IHC WB Rabbit IgG pThr286 Log in to see Polyclonal
2.9061391 ABIN1532284 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9061391 ABIN2704689 IHC WB Rabbit C-Term, pSer286 Log in to see Polyclonal
2.9061391 ABIN498398 WB Rabbit Log in to see Polyclonal 1
2.9061391 ABIN3181597 IHC WB Rabbit IgG Log in to see Polyclonal
2.9061391 ABIN2736604 IF IP IHC WB Rabbit IgG Log in to see Polyclonal
2.9061391 ABIN1871539 IHC WB Rabbit IgG Log in to see Polyclonal
2.9061391 ABIN4301536 ICC IF IP WB Rabbit IgG Log in to see Polyclonal

General

Antigen Cyclin D1 (CCND1) Antibodies
Epitope Middle Region
(79), (24), (21), (16), (14), (11), (11), (10), (9), (8), (8), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Pig (Porcine), Rabbit, Rat (Rattus), Yeast, Zebrafish
(488), (244), (236), (44), (21), (19), (19), (16), (15), (14), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(325), (204), (19), (4)
Clonality
Polyclonal
Conjugate This Cyclin D1 antibody is un-conjugated
(26), (23), (15), (10), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(447), (209), (133), (127), (117), (109), (109), (80), (51), (14), (13), (12), (8), (8), (7), (6), (5), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Cyclin D1 Antibody

Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN2779291) Images
Blocking Peptide Blocking peptide for this product available: ABIN974598
Sequence ACQEQIEALL ESSLRQAQQN MDPKAAEEEE EEEEEVDLAC TPTDVRDVDI
Predicted Reactivity Cow: 92%, Dog: 92%, Horse: 92%, Human: 100%, Pig: 91%, Rabbit: 83%, Rat: 85%, Yeast: 83%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against CCND1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CCND1

Target Details Cyclin D1

Product Details anti-Cyclin D1 Antibody Application Details Handling References for anti-Cyclin D1 antibody (ABIN2779291) Images back to top
Antigen
Alternative Name CCND1 (CCND1 Antibody Abstract)
Background CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: BCL1, D11S287E, PRAD1, U21B31
Protein Interaction Partner: CDKN1B, CDKN1A, CDK4, RELA, FBXO31, UBC, FBXO4, CAMK1, TP73, RB1, CDK2, HSPA8, POLR1B, MCM10, ORC4, LPL, BRINP1, CDK6, RABEP1, CARD11, CUL1, RBL2, UBTF, CCND1, ATF2, CDKN2A, FBXW8, BTRC, PPP1CC, PPP1CB, PSMA6, ITLN1, MCM7, MCM6, MCM3, MCM2, ELAVL1, PCNA,
Protein Size: 295
Molecular Weight 34 kDa
Gene ID 595
NCBI Accession NM_053056, NP_444284
UniProt P24385
Pathways PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling

Application Details

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Handling References for anti-Cyclin D1 antibody (ABIN2779291) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 295 AA

Restrictions For Research Use only

Handling

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details References for anti-Cyclin D1 antibody (ABIN2779291) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Cyclin D1 antibody (ABIN2779291)

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling Images back to top
Background publications

Kibel, Jin, Klim, Luly, A Roehl, Wu, Suarez: "Association between polymorphisms in cell cycle genes and advanced prostate carcinoma." in: The Prostate, Vol. 68, Issue 11, pp. 1179-86, 2008

Images

Product Details anti-Cyclin D1 Antibody Target Details Cyclin D1 Application Details Handling References for anti-Cyclin D1 antibody (ABIN2779291) back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin D1 (CCND1) (Middle Region) antibody (ABIN2779291) Lanes : Lane 1: 30ug PKO lysateLane 2: 30ug PKO + Doxor lysate Primary Antibody Dilu...
Immunohistochemistry (IHC) image for anti-Cyclin D1 (CCND1) (Middle Region) antibody (ABIN2779291) Rabbit Anti-CCND1 Antibody Catalog Number: ARP30163_P050 Formalin Fixed Paraffin Embe...
Western Blotting (WB) image for anti-Cyclin D1 (CCND1) (Middle Region) antibody (ABIN2779291) WB Suggested Anti-CCND1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive...