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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||325 Antibodies||138 Antibodies||115 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||98 Antibodies||35 Antibodies||27 Antibodies|
|Immunohistochemistry (IHC)||176 Antibodies||95 Antibodies||78 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||45 Antibodies|
|Antigen||Forkhead Box O1 (FOXO1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This FOXO1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-FOXO1 AntibodyTarget Details FOXO1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p-FKHR/FOXO1A protein, only when phosphorylated at Ser319.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details FOXO1Product Details anti-FOXO1 Antibody Application Details Handling Images back to top
|Alternative Name||FOXO1A (FOXO1 Antibody Abstract)|
|Background||The transcription factor forkhead in rhabdomyosarcoma (FKHR), which is inhibited by insulin and IGF-1, enhances transcription. FKHR has been implicated in alveolar rhabdomyosarcoma, a soft tissue tumor wherein a chromosomal translocation [t(2,12)(q35,q14)] occurs between the FKHR and PAX3 genes, resulting in a novel chimeric protein with abnormal levels of expression. FKHR becomes phosphorylated at Ser 319, Ser 256 and Thr 256 by protein kinase B (PKB) in a phosphoinsoditide 3 (PI 3)-kinase/Akt-dependent pathway, resulting in the inactivation and subsequent nuclear exit of FKHR. In addition, FKHR becomes phosphorylated at Ser 329, also resulting in decreased FKHR activity and diminished nuclear FKHR concentration. However, phosphorylation of FKHR at Ser 329 is not mediated by a PI 3-kinasedependent pathway, but by an alternate mechanism. Dualspecificity tyrosine-phosphorylated and regulated kinase 1A (DYRK1A), which co-localizes to the same region of the nucleus as FKHR, specifically phosphorylates FKHR at Ser 329 in rabbit skeletal muscle.Synonyms: FKHR, FOXO1, Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead in Rhabdomyosarcoma|
|Molecular Weight||approx. 70 to 82 kDa|
|Research Area||Cancer, Transcription Factors|
|Pathways||PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-FOXO1 Antibody Target Details FOXO1 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FOXO1 Antibody Target Details FOXO1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-FOXO1 Antibody Target Details FOXO1 Application Details Handling back to top