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anti-Human Glycogen Synthase 1 antibody for Immunoprecipitation

Recommended Glycogen Synthase 1 Antibody (supplied by: Log in to see )

Antigen
Glycogen Synthase 1 (Muscle) (GYS1) Antibodies
  • GSY
  • GYS
  • GYS1
  • GYS2
  • Gys3
  • MGS
  • zgc:63701
Epitope
pSer640
4
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
38
15
13
4
4
4
Host
Rabbit
33
5
Clonality
Polyclonal
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
36
24
15
8
6
4
4
2
Supplier
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Catalog No. ABIN1043894
$ 579.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008912129 ABIN1043895 IP IHC ELISA WB Rabbit IgG pSer640 Log in to see Polyclonal 1
0.0008912129 ABIN214350 IHC (p) IP ELISA WB Rabbit pSer640 Log in to see Polyclonal
0.0008912129 ABIN2470236 IP IHC ELISA WB Rabbit AA 249-265 Log in to see Polyclonal
0.0008912129 ABIN228217 IHC (p) IP WB Rabbit IgG pSer641 Log in to see Polyclonal
0.0008912129 ABIN230188 ICC FACS IHC (p) IP ELISA WB Rabbit IgG Log in to see
0.0008912129 ABIN1900885 IP WB Rabbit AA 625-675 Log in to see Polyclonal
0.0008912129 ABIN1900887 IP WB Rabbit AA 687-737 Log in to see Polyclonal
0.0008912129 ABIN2767888 IP IHC (p) IHC ELISA WB Rabbit pSer640 Log in to see Polyclonal

Top referenced anti-Glycogen Synthase 1 antibody for Immunoprecipitation

Similar anti-Glycogen Synthase 1 Antibodies

Application / Reactivity Human
ELISA 25 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 37 Antibodies

General

Antigen Glycogen Synthase 1 (Muscle) (GYS1) Antibodies
Epitope pSer640
(4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(38), (15), (13), (4), (4), (4)
Host Rabbit
(33), (5)
Clonality
Polyclonal
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(36), (24), (15), (8), (6), (4), (4), (2)
Pubmed 1 reference available
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Product Details anti-Glycogen Synthase 1 Antibody

Target Details Glycogen Synthase 1 Application Details Handling References for anti-Glycogen Synthase 1 Antibody (ABIN1043894) Images
Specificity This affinity purified antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS640 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.
Characteristics Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephos-phorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3β that mainly functions to phos-phorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.
Sterility Sterile filtered
Immunogen Human Muscle Glycogen Synthase phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
Isotype IgG

Target Details Glycogen Synthase 1

Product Details anti-Glycogen Synthase 1 Antibody Application Details Handling References for anti-Glycogen Synthase 1 Antibody (ABIN1043894) Images back to top
Antigen
Alternative Name Muscle Glycogen Synthase (GYS1 Antibody Abstract)
Background Anti-Glycogen Synthase 1 pS640 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephos-phorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3β that mainly functions to phos-phorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.
Synonyms: Glycogen antibody, Glycogen synthase 1 (muscle) antibody, Glycogen synthase 1 antibody, Glycogen synthase1 antibody, GSY antibody, GYS 1 antibody, GYS antibody, GYS1 antibody, Starchsynthase muscle antibody
Gene ID 2997
UniProt P13807
Pathways PI3K-Akt Signaling, AMPK Signaling

Application Details

Product Details anti-Glycogen Synthase 1 Antibody Target Details Glycogen Synthase 1 Handling References for anti-Glycogen Synthase 1 Antibody (ABIN1043894) Images back to top
Application Notes This phospho specific polyclonal antibody was tested by immunoblotting and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Comment

Gene Name: GYS1

Restrictions For Research Use only

Handling

Product Details anti-Glycogen Synthase 1 Antibody Target Details Glycogen Synthase 1 Application Details References for anti-Glycogen Synthase 1 Antibody (ABIN1043894) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-Glycogen Synthase 1 Antibody (ABIN1043894)

Product Details anti-Glycogen Synthase 1 Antibody Target Details Glycogen Synthase 1 Application Details Handling Images back to top
Product cited in:

Kelsall, Voss, Munro, Cuthbertson, Cohen: "R3F, a novel membrane-associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals." in: Journal of neurochemistry, Vol. 118, Issue 4, pp. 596-610, 2011