Human Glycogen Synthase 1 ELISA Kit

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Antigen
Glycogen Synthase 1 (Muscle) (GYS1) ELISA Kits
  • GYS1
  • zgc:63701
  • GSY
  • GYS
  • GYS2
  • Gys3
  • MGS
  • glycogen synthase 1 (muscle)
  • glycogen synthase 1, muscle
  • GYS1
  • gys1
  • Gys1
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
2
1
1
Method Type
Sandwich ELISA
Detection Range
1.25-80 ng/mL
Minimum Detection Limit
1.25 ng/mL
Application
ELISA
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Catalog No. ABIN4993483
$ 638.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN2684404 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see

Similar Glycogen Synthase 1 ELISA Kits

Application / Reactivity Mouse (Murine) Rat (Rattus)
ELISA 1 ELISA Kits 2 ELISA Kits

General

Antigen Glycogen Synthase 1 (Muscle) (GYS1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(2), (1), (1)
Method Type Sandwich ELISA
Detection Range 1.25-80 ng/mL
Minimum Detection Limit 1.25 ng/mL
Application ELISA
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Product Details Glycogen Synthase 1 ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GYS1 in human serum, plasma and other biological fluids
Sample Type Plasma, Serum, Biological Fluids
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This kit recognizes natural and recombinantHumanGYS1. No significant cross-reactivity or interference between HumanGYS1 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanGYS1 and all the analogues.
Sensitivity 0.75 ng/mL
Components Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis
Material not included Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Target details

Product Details Glycogen Synthase 1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Glycogen Synthase 1, Muscle (GYS1) (GYS1 ELISA Kit Abstract)
Pathways PI3K-Akt Signaling, AMPK Signaling, Cellular Glucan Metabolic Process

Application Details

Product Details Glycogen Synthase 1 ELISA Kit Target details Handling Images back to top
Comment

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Sample Volume 100 µL
Plate Pre-coated
Protocol This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GYS1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GYS1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GYS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GYS1.You can calculate the concentration of GYS1in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
Restrictions For Research Use only

Handling

Product Details Glycogen Synthase 1 ELISA Kit Target details Application Details Images back to top
Handling Advice All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
Storage 4 °C/-20 °C
Storage Comment The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.