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|Application / Reactivity||Human|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||33 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||45 Antibodies|
|Immunohistochemistry (IHC)||42 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||26 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Western Blotting (WB)||137 Antibodies|
|Antigen||Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) Antibodies|
|Conjugate||This GRIA1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-GRIA1 AntibodyTarget Details GRIA1 Application Details Handling Images
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues near N-terminus of human Glutamate receptor 1 was used as immunogen.|
Target Details GRIA1Product Details anti-GRIA1 Antibody Application Details Handling Images back to top
|Alternative Name||Glutamate receptor 1 / GLUR1 (GRIA1 Antibody Abstract)|
Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervoussystem. Glutamate receptors are classified on the basis of their activation by differentagonists (1-3). GluR1, human glutamate receptor type 1, is an integral membraneprotein that is widely expressed in the human brain. The postsynaptic actions ofglutamic acid are mediated by a variety of receptors that are named according to theirselective agonists. GluR1 is known to bind a kainate subtype of agonist. It has beenfound that malfunctioning of the glutamatergic system may result in certain braindisorders and neurodegeneration (3). Alternate Names: anti-GPRC1A antibody, anti-GRM 1 antibody, anti-GRM1 alpha antibody, anti-GRM1antibody, anti-GRM1A antibody, anti-Metabotropic glutamate receptor 1 antibody,anti-mGlu1 antibody, anti-mGluR1 alpha antibody, anti-mGluR1 antibody, anti-mGluR1Aantibody.
Gene Symbol: GRIA1
|Gene ID||2890, 14799, 50592|
Application DetailsProduct Details anti-GRIA1 Antibody Target Details GRIA1 Handling Images back to top
|Application Notes||Recommended dilutions: Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunoprecipitation 1:30, Western Blot 1:1000|
Protocol specific for Glutamate Receptor 1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-GRIA1 Antibody Target Details GRIA1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-GRIA1 Antibody Target Details GRIA1 Application Details Handling back to top