|Application / Reactivity||Mouse (Murine)||Rat (Rattus)||Human||Non Human Primates|
|Western Blotting (WB)||108 Antibodies||144 Antibodies||137 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||26 Antibodies||19 Antibodies||20 Antibodies|
|Immunohistochemistry (IHC)||67 Antibodies||42 Antibodies||46 Antibodies||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies||4 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||47 Antibodies||47 Antibodies|
|Antigen||Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) Antibodies|
|Reactivity||Human, Mouse (Murine), Non Human Primates, Rat (Rattus) Alternatives|
|Conjugate||This GRIA1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
|15 references available|
|Supplier||Log in to see|
Product Details anti-GRIA1 AntibodyTarget Details GRIA1 Application Details Handling References for anti-GRIA1 Antibody (ABIN372653) Images
|Specificity||Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is s completely eliminated by treatment with lambda-Ptase.|
Species reactivity (expected):Human, Mouse and non-Human Primates.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1.|
Target Details GRIA1Product Details anti-GRIA1 Antibody Application Details Handling References for anti-GRIA1 Antibody (ABIN372653) Images back to top
|Alternative Name||Glutamate Receptor 1 / GLUR1 (GRIA1 Antibody Abstract)|
|Background||The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990, Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993, Mammen et al., 1999, Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory (Soderling and Derkach, 2000).Synonyms: AMPA-selective glutamate receptor 1, AMPA1, GLUH1, GRIA1, GluR-1, GluR-A, GluR-K1, Glutamate receptor ionotropic|
Application DetailsProduct Details anti-GRIA1 Antibody Target Details GRIA1 Handling References for anti-GRIA1 Antibody (ABIN372653) Images back to top
Western blot: 1/1000. Immunofluorescence. Immunohistochemitsry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GRIA1 Antibody Target Details GRIA1 Application Details References for anti-GRIA1 Antibody (ABIN372653) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C/-80 °C|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C or (in aliquots) at -80 °C for long term.|
References for anti-GRIA1 Antibody (ABIN372653)Product Details anti-GRIA1 Antibody Target Details GRIA1 Application Details Handling Images back to top
|Product cited in:||
Shen, Fu, Cheng et al.: "Melanocortin-4 receptor regulates hippocampal synaptic plasticity through a protein kinase A-dependent mechanism." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 464-72, 2013 (PubMed).
Santini, Feyder, Gangarossa et al.: "Dopamine- and cAMP-regulated phosphoprotein of 32-kDa (DARPP-32)-dependent activation of extracellular signal-regulated kinase (ERK) and mammalian target of rapamycin complex 1 (mTORC1) signaling in ..." in: The Journal of biological chemistry, Vol. 287, Issue 33, pp. 27806-12, 2012 (PubMed).
Sears, Liu, Narayanan et al.: "Regulation of nucleus accumbens activity by the hypothalamic neuropeptide melanin-concentrating hormone." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8263-73, 2010 (PubMed).
Rebholz, Nishi, Liebscher et al.: "CK2 negatively regulates Galphas signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 14096-101, 2009 (PubMed).
Santini, Heiman, Greengard et al.: "Inhibition of mTOR signaling in Parkinson's disease prevents L-DOPA-induced dyskinesia." in: Science signaling, Vol. 2, Issue 80, pp. ra36, 2009 (PubMed).
Zheng, Keifer: "PKA has a critical role in synaptic delivery of GluR1- and GluR4-containing AMPARs during initial stages of acquisition of in vitro classical conditioning." in: Journal of neurophysiology, Vol. 101, Issue 5, pp. 2539-49, 2009 (PubMed).
Meyer, Richer, Benkovic et al.: "Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18561-6, 2008 (PubMed).
Nishi, Kuroiwa, Miller et al.: "Distinct roles of PDE4 and PDE10A in the regulation of cAMP/PKA signaling in the striatum." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 42, pp. 10460-71, 2008 (PubMed).
Davies, Goebel-Goody, Coultrap et al.: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, 2008 (PubMed).
Soderling, Derkach: "Postsynaptic protein phosphorylation and LTP." in: Trends in neurosciences, Vol. 23, Issue 2, pp. 75-80, 2000 (PubMed).
Mammen, Kameyama, Roche et al.: "Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin-dependent kinase II." in: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32528-33, 1998 (PubMed).
Roche, OBrien, Mammen et al.: "Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit." in: Neuron, Vol. 16, Issue 6, pp. 1179-88, 1996 (PubMed).
McGlade-McCulloh, Yamamoto, Tan et al.: "Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II." in: Nature, Vol. 362, Issue 6421, pp. 640-2, 1993 (PubMed).
Hollmann, Heinemann: "Cloned glutamate receptors." in: Annual review of neuroscience, Vol. 17, pp. 31-108, 1994 (PubMed).
Keinänen, Wisden, Sommer et al.: "A family of AMPA-selective glutamate receptors." in: Science (New York, N.Y.), Vol. 249, Issue 4968, pp. 556-60, 1990 (PubMed).
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