Rabbit GRIA1 ELISA Kit

Recommended GRIA1 Product (supplied by: Log in to see )

Antigen
Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) ELISA Kits
  • GLUH1
  • GLUR1
  • GLURA
  • GluA1
  • HBGR1
  • gluR-A
  • 2900051M01Rik
  • Glr-1
  • Glr1
  • GluR-A
  • GluRA
  • Glur-1
  • Glur1
  • HIPA1
  • gluR-K1
  • GRIA1
  • glur1
  • glutamate ionotropic receptor AMPA type subunit 1
  • glutamate receptor, ionotropic, AMPA1 (alpha 1)
  • glutamate receptor, ionotropic, AMPA 1
  • glutamate receptor, ionotropic, AMPA 1 L homeolog
  • GluR1 protein
  • Glutamate receptor 1
  • GRIA1
  • Gria1
  • gria1
  • gria1.L
  • glur1
  • glr-1
Reactivity
Rabbit
Alternatives
Kits with alternative reactivity to:
18
13
8
2
1
1
1
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
0.15 ng/mL - 10 ng/mL
Minimum Detection Limit
0.15 ng/mL
Application
ELISA
Options
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Catalog No. ABIN5564709
$ 640.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN5665048 Sandwich ELISA Biological Fluids, Cell Lysate, Tissue Homogenate 31.2 pg/mL - 2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN1746817 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see

General

Antigen Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) ELISA Kits
Reactivity Rabbit
Kits with alternative reactivity to:
(18), (13), (8), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.15 ng/mL - 10 ng/mL
Minimum Detection Limit 0.15 ng/mL
Application ELISA
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Product Details GRIA1 ELISA Kit

Target details Application Details Handling Images
Sample Type Biological Fluids, Cell Lysate, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity

This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1).
No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) and analogues was observed.

Sensitivity 0.055 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Plate sealer for 96 wells
  • Standard Diluent
  • Assay Diluent A
  • Assay Diluent B
  • Stop Solution
  • Standard
  • Detection Reagent A
  • Detection Reagent B
  • TMB Substrate
  • Wash Buffer (30 x concentrate)
  • Instruction manual
Material not included
  • Microplate reader with 450 nm filter.
  • Precision single or multi-channel pipettes and disposable tips.
  • Eppendorf Tubes for diluting samples.
  • Deionized or distilled water.
  • Absorbent paper for blotting the microtiter plate.
  • Container for Wash Solution

Target details

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Antigen
Pathways PI3K-Akt Signaling

Application Details

Product Details GRIA1 ELISA Kit Target details Handling Images back to top
Assay Time 3 h
Plate Pre-coated
Protocol The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure
  1. Prepare all reagents, samples and standards,
  2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
  3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
  4. Aspirate and wash 3 times,
  5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
  6. Aspirate and wash 5 times,
  7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
  8. Add 50μL Stop Solution. Read at 450nm immediately.
Assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions For Research Use only

Handling

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Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Storage 4 °C
Storage Comment
  • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
  • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
    Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
  • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
Expiry Date 6 months

Images

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Supplier Images
ELISA image for Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) ELISA Kit (ABIN5564709) Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) ELISA Kit