|Application / Reactivity||Human|
|Confocal Microscopy (CM)||2 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||12 Antibodies|
|Immunohistochemistry (IHC)||36 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||21 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Western Blotting (WB)||106 Antibodies|
|Antigen||Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) Antibodies|
|Epitope||C-Term, AA 840-886 Alternatives|
|Conjugate||This GRIA2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-GRIA2 AntibodyTarget Details GRIA2 Application Details Handling References for anti-GRIA2 Antibody (ABIN493016) Images
|Specificity||This antibody detects a single band of approximately 106 kDa in GluR2 samples.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human|
|Immunogen||Synthetic peptide corresponding to C-term of GluR2 (aa 840-886)|
Target Details GRIA2Product Details anti-GRIA2 Antibody Application Details Handling References for anti-GRIA2 Antibody (ABIN493016) Images back to top
|Alternative Name||Glutamate Receptor 2 / GLUR2 (GRIA2 Antibody Abstract)|
|Background||Ionotropic Glutamate Receptor 2 (GluR2) is a predominant excitatory neurotransmitter in mammalian brain located at human chromosome 4q32-33 (1). It belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors (2). GluR2 plays a crucial role in controlling the calcium permeability of AMPA receptors and therefore, its presence or absence greatly influences AMPA receptor properties and synaptic functions. GluR2 mRNA ordinarily undergoes post-transcriptional editing which is essential for brain function. AMPA receptors containing edited GluR2 have low conductance, low calcium permeability and confer a linear current-voltage (I-V) relationship. AMPA receptors lacking an edited GluR2 subunit have high calcium permeability and also exhibit inward rectifying I-V relationship (3). Defective GluR2 RNA editing might also play a role in the development of amyotrophic lateral sclerosis (ALS) (4). GluR2 is among the most common and abundant receptor in striatum and projection neurons. In rats, all striatal projection neurons express GluR2 (5). It is highly concentrated on the postsynaptic neuronal membranes of the superficial dorsal horn and its removal might contribute to spinal central sensitization in persistent inflammatory pain (6). It is expressed in cortex, hippocampus and cerebellum. It is approximately a 106 kDa protein (886 amino acids).Synonyms: AMPA-selective glutamate receptor 2, AMPA2, GRIA2, GluR-B, GluR-K2, Glutamate receptor ionotropic|
Application DetailsProduct Details anti-GRIA2 Antibody Target Details GRIA2 Handling References for anti-GRIA2 Antibody (ABIN493016) Images back to top
ELISA: < 1: 10,000. Western blot: > 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GRIA2 Antibody Target Details GRIA2 Application Details References for anti-GRIA2 Antibody (ABIN493016) Images back to top
|Concentration||0.64 - 0.72 mg/mL|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store (in aliquots) at -20 °C.|
References for anti-GRIA2 Antibody (ABIN493016)Product Details anti-GRIA2 Antibody Target Details GRIA2 Application Details Handling Images back to top
Park, Voitenko, Petralia et al.: "Persistent inflammation induces GluR2 internalization via NMDA receptor-triggered PKC activation in dorsal horn neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 10, pp. 3206-19, 2009 (PubMed).
Deng, Xie, Wang et al.: "Differential localization of the GluR1 and GluR2 subunits of the AMPA-type glutamate receptor among striatal neuron types in rats." in: Journal of chemical neuroanatomy, Vol. 33, Issue 4, pp. 167-92, 2007 (PubMed).
Gryder, Castaneda, Rogawski: "Evidence for low GluR2 AMPA receptor subunit expression at synapses in the rat basolateral amygdala." in: Journal of neurochemistry, Vol. 94, Issue 6, pp. 1728-38, 2005 (PubMed).
Kawahara, Ito, Sun et al.: "Glutamate receptors: RNA editing and death of motor neurons." in: Nature, Vol. 427, Issue 6977, pp. 801, 2004 (PubMed).
Sun, Ferrer-Montiel, Montal: "Primary structure and functional expression of the AMPA/kainate receptor subunit 2 from human brain." in: Neuroreport, Vol. 5, Issue 4, pp. 441-4, 1994 (PubMed).