|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies||4 Antibodies||4 Antibodies|
|Western Blotting (WB)||229 Antibodies||133 Antibodies||106 Antibodies||5 Antibodies|
|Immunoprecipitation (IP)||41 Antibodies||37 Antibodies||36 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||41 Antibodies||28 Antibodies||24 Antibodies|
|Immunohistochemistry (IHC)||109 Antibodies||64 Antibodies||46 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Antigen||Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This GSK3 alpha antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-GSK3 alpha AntibodyTarget Details GSK3 alpha Application Details Handling Images
|Specificity||This antibody specifically recognizes GSK3alpha phosphorylated at serine 21 (Western blot: 51 kDa) but does not crossreact with the homologous phosphorylation site in GSK3beta (phospho-Ser 9).|
|Characteristics||Synonyms: Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A|
|Purification||Size exclusion chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic phosphopeptide conjugated to KLH.|
Target Details GSK3 alphaProduct Details anti-GSK3 alpha Antibody Application Details Handling Images back to top
|Alternative Name||GSK3 alpha (GSK3a Antibody Abstract)|
|Background||Glycogen Synthase Kinase 3 (GSK3) exists in two isoforms, alpha and beta (GSK3alpha, GSK3beta). GSK3 is an active kinase that becomes phosphorylated by PKB/akt upon growth factor stimulation. Phosphorylation of GSK3alpha at serine 21 or GSK3beta at serine 9, respectively, leads to inhibition of kinase activity of the GSK3.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha|
|Research Area||Cardiovascular, Hypertrophy, Atherosclerosis, Wnt, Kinases/Phosphatases, Wnt Signaling|
|Pathways||PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process|
Application DetailsProduct Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.0 5g/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Handling back to top