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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies|
|Immunofluorescence (IF)||36 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||12 Antibodies|
|Immunohistochemistry (IHC)||103 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||23 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies|
|Western Blotting (WB)||207 Antibodies|
|Antigen||Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies|
|Conjugate||This GSK3 alpha antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-GSK3 alpha AntibodyTarget Details GSK3 alpha Application Details Handling References for anti-GSK3 alpha Antibody (ABIN969179) Images
|Immunogen||Purified recombinant fragment of GSK3 alpha expressed in E. coli.|
Target Details GSK3 alphaProduct Details anti-GSK3 alpha Antibody Application Details Handling References for anti-GSK3 alpha Antibody (ABIN969179) Images back to top
|Alternative Name||GSK3 alpha (GSK3a Antibody Abstract)|
|Background||GSK3 alpha (Glycogen synthase kinase 3 alpha), with 483-amino acid protein (about 53 kDa), belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily,GSK3 subsubfamily, which also includes GSK3 beta.GSK3 is a multifunctional serine/threonine kinase that is usually inactivated by serine phosphorylation in response to extracellular cues. GSK3 is a key regulator of numerous signalling pathways, including cellular responses to Wnt, receptor tyrosine kinases and G-protein-coupled receptors and is involved in a wide range of cellular processes, ranging from glycogen metabolism to cell cycle regulation and proliferation.GSK3 alpha is implicated in the control of several regulatory proteins including glycogen synthase and transcription factors.|
|Research Area||Cardiovascular, Hypertrophy, Atherosclerosis, Wnt, Kinases/Phosphatases|
|Pathways||PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Handling References for anti-GSK3 alpha Antibody (ABIN969179) Images back to top
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details References for anti-GSK3 alpha Antibody (ABIN969179) Images back to top
|Buffer||Ascitic fluid containing 0.03 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 4 °C or at -20 °C for long term.|
References for anti-GSK3 alpha Antibody (ABIN969179)Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Handling Images back to top
|Product cited in:||
Sayas, Ariaens, Ponsioen et al.: "GSK-3 is activated by the tyrosine kinase Pyk2 during LPA1-mediated neurite retraction." in: Molecular biology of the cell, Vol. 17, Issue 4, pp. 1834-44, 2006 (PubMed).
Bradley, Currie, MacMillan et al.: "Cyclic ADP-ribose increases Ca2+ removal in smooth muscle." in: Journal of cell science, Vol. 116, Issue Pt 21, pp. 4291-306, 2003 (PubMed).
Doble, Woodgett: "GSK-3: tricks of the trade for a multi-tasking kinase." in: Journal of cell science, Vol. 116, Issue Pt 7, pp. 1175-86, 2003 (PubMed).
ImagesProduct Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Handling References for anti-GSK3 alpha Antibody (ABIN969179) back to top