You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human GSK3 alpha antibody for Western Blotting

Recommended GSK3 alpha Antibody (supplied by: Log in to see )

Antigen
Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
  • GSK3A
  • CG5182
  • Dmel\\CG5182
  • GSK3a
  • 2700086H06Rik
  • xgsk3
  • GSK-3[a]
  • GSK-3[b]
  • GSK3
  • gsk3a
  • glycogen synthase kinase 3 alpha
  • CG5182 gene product from transcript CG5182-RA
  • glycogen synthase kinase 3 alpha b
  • GSK3A
  • gsk3a
  • Bm1_31380
  • Pk34A
  • Gsk3a
  • LOC100339192
  • gsk3ab
Epitope
AA 16-25, pSer21
65
34
25
15
10
8
7
6
6
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
269
155
129
7
7
5
4
4
2
1
1
1
Host
Rabbit
202
91
3
Clonality
Polyclonal
Conjugate
This GSK3 alpha antibody is un-conjugated
20
11
10
7
6
6
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
256
129
127
46
44
42
25
13
7
5
3
3
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN117908
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0741942 ABIN2792115 WB Rabbit N-Term Log in to see Polyclonal 1
3.0741942 ABIN264482 EIA IP WB Mouse IgG2a Log in to see 2
3.0741942 ABIN492620 WB Mouse IgG1 pTyr278, pTyr215 Log in to see 6D3
3.0741942 ABIN1724667 ELISA WB Mouse IgG1 Log in to see 6G12C2 3
3.0741942 ABIN1724666 ICC ELISA WB Mouse IgG1 Log in to see 9D5G1 3
3.0741942 ABIN126804 EIA WB Mouse IgG1 pSer21 Log in to see 9B8
3.0741942 ABIN126803 WB Mouse IgG1 Log in to see 10C1
3.0741942 ABIN969179 IHC ELISA WB Mouse Log in to see 6G2F1 3
3.0741942 ABIN1531854 IHC ELISA WB Rabbit IgG pSer21 Log in to see Polyclonal
3.0741942 ABIN4900770 FACS ICC WB Rabbit IgG Log in to see Polyclonal 1
3.0741942 ABIN4900620 FACS ICC WB Rabbit IgG pSer9 Log in to see Polyclonal 3
3.0741942 ABIN498788 IHC (p) WB Rabbit pSer21 Log in to see Polyclonal
3.0741942 ABIN1107446 WB Mouse IgG1 Log in to see 6G12C2 3
3.0741942 ABIN1107445 IF WB Mouse IgG1 Log in to see 9D5G1 3
3.0741942 ABIN3182021 ELISA IHC IP WB Rabbit IgG pSer21 Log in to see Polyclonal
3.0741942 ABIN3184955 ELISA IHC IP WB Rabbit IgG Ser45 Log in to see Polyclonal
3.0741942 ABIN2707539 IC IF IP IHC WB Rabbit N-Term, pSer21 Log in to see Polyclonal
3.0741942 ABIN1870216 IF IHC WB Rabbit IgG pSer21 Log in to see Polyclonal
3.0741942 ABIN498100 IHC (p) WB Rabbit Log in to see Polyclonal
3.0741942 ABIN1872906 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-GSK3 alpha antibody for Western Blotting

Similar anti-GSK3 alpha Antibodies

Application / Reactivity Human
ELISA 106 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 109 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies
Immunoprecipitation (IP) 41 Antibodies
Western Blotting (WB) 227 Antibodies

General

Antigen Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
Epitope AA 16-25, pSer21
(65), (34), (25), (15), (10), (8), (7), (6), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(269), (155), (129), (7), (7), (5), (4), (4), (2), (1), (1), (1)
Host Rabbit
(202), (91), (3)
Clonality
Polyclonal
Conjugate This GSK3 alpha antibody is un-conjugated
(20), (11), (10), (7), (6), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(256), (129), (127), (46), (44), (42), (25), (13), (7), (5), (3), (3), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-GSK3 alpha Antibody

Target Details GSK3 alpha Application Details Handling References for anti-GSK3 alpha Antibody (ABIN117908) Images
Sequence Protein Sequence: Human GSK3 alpha, 483 aa, predicted MW 51.0 kDa 1 msgggpsggg pggsgrarts sfaepggggg gggggpggsa sgpggtgggk asvgamgggv 61 gasssgggpg gsggggsggp gagtsfpppg vklgrdsgkv ttvvatlgqg persqevayt 121 dikvigngsf gvvyqarlae trelvaikkv lqdkrfknre lqimrkldhc nivrlryffy 181 ssgekkdely lnlvleyvpe tvyrvarhft kakltipily vkvymyqlfr slayihsqgv 241 chrdikpqnl lvdpdtavlk lcdfgsakql vrgepnvsyi csryyrapel ifgatdytss 301 idvwsagcvl aelllgqpif pgdsgvdqlv eiikvlgtpt reqiremnpn ytefkfpqik 361 ahpwtkvfks rtppeaialc sslleytpss rlspleacah sffdelrclg tqlpnnrplp 421 plfnfsagel siqpslnail ipphlrspag tttltpssqa ltetptssdw qstdatptlt 481 nss
Specificity This affinity-purified antibody is directed against the phosphorylated form of human GSK3A at the pS 21 residue. The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody reacts with phosphorylated pS21 of human GSK3A. Reactivity with non-phosphorylated human GSK3A is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100 % homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined.
Purification Immunoaffinity chromatography.
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 16-25 of human GSK3 alpha.

Target Details GSK3 alpha

Product Details anti-GSK3 alpha Antibody Application Details Handling References for anti-GSK3 alpha Antibody (ABIN117908) Images back to top
Antigen
Alternative Name GSK3 alpha (GSK3a Antibody Abstract)
Background Glycogen synthase kinase 3 alpha (GSK3A) belongs to the ser/thr family of protein kinases, Cdc2/cdkx subfamily, gsk-3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c-jun. GSK3A is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta (GSK3B), show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha
Gene ID 2931
NCBI Accession NP_063937
UniProt P49840
Research Area Cardiovascular, Hypertrophy, Atherosclerosis, Wnt, Kinases/Phosphatases, Wnt Signaling
Pathways PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Handling References for anti-GSK3 alpha Antibody (ABIN117908) Images back to top
Application Notes This affinity-purified antibody has been tested for use in ELISA (1: 2,000-1: 10,000) and byWestern blot (1: 500-1: 3,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 52 kDa in size corresponding to GSK3A by Westernblotting in the appropriate cell lysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated form of theimmunizing peptide. This antibody is phosphor specific for pS21 of GSK3A.
Restrictions For Research Use only

Handling

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details References for anti-GSK3 alpha Antibody (ABIN117908) Images back to top
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-GSK3 alpha Antibody (ABIN117908)

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Handling Images back to top
Product cited in:

Wang, He, Wang, Wang, Zhai, Zhou, Zhang, Zhai, Feng, Han: "[Impact of Donor KIR2DS5 Genotype on Outcome Following Haploidentical Hematopoietic Stem Cell Transplantation.]" in: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 1, pp. 111-5, 2008

Background publications

Gaster, Brusgaard, Handberg, Højlund, Wojtaszewski, Beck-Nielsen: "The primary defect in glycogen synthase activity is not based on increased glycogen synthase kinase-3alpha activity in diabetic myotubes." in: Biochemical and biophysical research communications, Vol. 319, Issue 4, pp. 1235-40, 2004

Barry, Graham, Fry, Gibbins: "Regulation of glycogen synthase kinase 3 in human platelets: a possible role in platelet function?" in: FEBS letters, Vol. 553, Issue 1-2, pp. 173-8, 2003

Nikoulina, Ciaraldi, Mudaliar, Carter, Johnson, Henry: "Inhibition of glycogen synthase kinase 3 improves insulin action and glucose metabolism in human skeletal muscle." in: Diabetes, Vol. 51, Issue 7, pp. 2190-8, 2002

He, Saint-Jeannet, Woodgett, Varmus, Dawid: "Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos." in: Nature, Vol. 374, Issue 6523, pp. 617-22, 1995

Images

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Handling References for anti-GSK3 alpha Antibody (ABIN117908) back to top
Supplier Images
Western Blotting (WB) image for anti-GSK3 alpha antibody (Glycogen Synthase Kinase 3 alpha) (AA 16-25) (ABIN117908) Western blot using Rockland's Affinity Purified anti-GSK3A antibody shows detection o...