anti-Rat (Rattus) GSK3 alpha antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended GSK3 alpha Antibody (supplied by: Log in to see )

Antigen
Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
  • GSK3A
  • CG5182
  • Dmel\\CG5182
  • GSK3a
  • 2700086H06Rik
  • xgsk3
  • GSK-3[a]
  • GSK-3[b]
  • GSK3
  • gsk3a
  • glycogen synthase kinase 3 alpha
  • CG5182 gene product from transcript CG5182-RA
  • glycogen synthase kinase 3 alpha b
  • GSK3A
  • gsk3a
  • Bm1_31380
  • Pk34A
  • Gsk3a
  • LOC100339192
  • gsk3ab
Epitope
pSer21
68
36
25
15
11
9
7
7
6
5
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
310
161
133
7
7
6
4
4
3
1
1
1
1
Host
Rabbit
217
119
3
Clonality
Polyclonal
Conjugate
This GSK3 alpha antibody is un-conjugated
24
13
12
7
6
6
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
297
152
138
58
50
48
32
13
7
7
5
4
3
1
Supplier
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Catalog No. ABIN498788
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.671195 ABIN498100 IHC (p) WB Rabbit Log in to see Polyclonal
13.671195 ABIN407681 IHC (p) IP IHC WB Rabbit N-Term Log in to see Polyclonal
13.671195 ABIN498879 IHC (p) WB Rabbit Log in to see Polyclonal
13.671195 ABIN4316346 IHC IHC (p) IP WB Rabbit pSer21 Log in to see Polyclonal
1 ABIN197104 IF IHC (p) WB Rabbit Ser21 Log in to see Polyclonal 5
1 ABIN265449 EIA IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN708116 IF (p) IHC (p) WB Rabbit IgG AA 1-50, pSer21 Log in to see Polyclonal
1 ABIN318029 IHC (p) IP WB Rabbit pSer21 Log in to see Polyclonal
1 ABIN708125 IHC (p) WB HRP Rabbit IgG AA 1-50, pSer21 Log in to see Polyclonal
1 ABIN708118 IHC (p) WB Biotin Rabbit IgG AA 1-50, pSer21 Log in to see Polyclonal
1 ABIN5552462 IHC (p) Rabbit C-Term Log in to see Polyclonal
1 ABIN2888428 IHC (p) IP ELISA WB Rabbit IgG pSer21 Log in to see Polyclonal
1 ABIN127158 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN257307 ICC IF IHC IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN1822506 IHC (p) WB Rabbit IgG pSer21 Log in to see Polyclonal
1 ABIN2891658 IHC (p) WB Rabbit Gly120, Gly273 Log in to see Polyclonal
1 ABIN2893210 IHC (p) IP WB Rabbit pSer21 Log in to see Polyclonal
1 ABIN2883844 ELISA IHC (p) IP WB Rabbit IgG AA 10-59 Log in to see Polyclonal
1 ABIN2891434 IHC (p) IP WB Rabbit Gly15 Log in to see Polyclonal
1 ABIN230190 IHC (p) ELISA WB Rabbit IgG pSer21 Log in to see

General

Antigen Glycogen Synthase Kinase 3 alpha (GSK3a) Antibodies
Epitope pSer21
(68), (36), (25), (15), (11), (9), (7), (7), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(310), (161), (133), (7), (7), (6), (4), (4), (3), (1), (1), (1), (1)
Host Rabbit
(217), (119), (3)
Clonality
Polyclonal
Conjugate This GSK3 alpha antibody is un-conjugated
(24), (13), (12), (7), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(297), (152), (138), (58), (50), (48), (32), (13), (7), (7), (5), (4), (3), (1)
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Product Details anti-GSK3 alpha Antibody

Target Details GSK3 alpha Application Details Handling Images
Specificity This antibody detects endogenous levels of p-GSK3alpha protein only when phosphorylated at Ser21.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details GSK3 alpha

Product Details anti-GSK3 alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name GSK3 alpha (GSK3a Antibody Abstract)
Background Glycogen synthase kinase-3 α and β (GSK-3alpha, beta) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3beta activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFalpha in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3alpha and GSK-3beta induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3alpha or Ser 9 of GSK-3beta and inactivates both forms.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha
Molecular Weight approx. 46,51 kDa
Gene ID 2931
NCBI Accession NP_063937
UniProt P49840
Research Area Cardiovascular, Hypertrophy, Atherosclerosis, Wnt, Kinases/Phosphatases, Wnt Signaling
Pathways PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunoprecipitation: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-GSK3 alpha Antibody Target Details GSK3 alpha Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer21) antibody (ABIN498788) Immunohistochemistry (IHC) analyzes of p-GSK3alpha antibody in paraffin-embedd...
Western Blotting (WB) image for anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer21) antibody (ABIN498788) Western blot (WB) analysis of p-GSK3alpha antibody in extracts from ovary cancer.