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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||16 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||66 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||20 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||134 Antibodies|
|Antigen||Hexokinase 2 (HK2) Antibodies|
|Epitope||AA 1-917, N-Term Alternatives|
|Conjugate||This Hexokinase 2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-Hexokinase 2 AntibodyTarget Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN263918) Images
|Specificity||This antibody detects Hexokinase 1, 2.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Characteristics||Synonyms: Hexokinase type II, Muscle form hexokinase, HK2, HKII|
|Purification||Protein-G affinity chromatography|
|Immunogen||Recombinant human Hexokinase 2 (1-917aa) purified from E. coli|
Target Details Hexokinase 2Product Details anti-Hexokinase 2 Antibody Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN263918) Images back to top
|Alternative Name||Hexokinase-2 (HK2 Antibody Abstract)|
|Background||Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.Synonyms: HK2, HKII, Hexokinase type II, Muscle form hexokinase|
Application DetailsProduct Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Handling References for anti-Hexokinase 2 Antibody (ABIN263918) Images back to top
ELISA. Western blot: 1: 1,000 approx. 1: 3,000, 1: 2,000 as starting dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details References for anti-Hexokinase 2 Antibody (ABIN263918) Images back to top
|Buffer||Phosphate-Buffered Saline ( pH 7.4) with 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-Hexokinase 2 Antibody (ABIN263918)Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling Images back to top
Wilson: "Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function." in: The Journal of experimental biology, Vol. 206, Issue Pt 12, pp. 2049-57, 2003
Furuta, Nishi, Le Beau, Fernald, Yano, Bell: "Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization." in: Genomics, Vol. 36, Issue 1, pp. 206-9, 1997
ImagesProduct Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN263918) back to top