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anti-Human Hexokinase 2 antibody for Enzyme Immunoassay

Recommended Hexokinase 2 Antibody (supplied by: Log in to see )

Antigen
Hexokinase 2 (HK2) Antibodies
  • HK2
  • HKII
  • HXK2
  • hexokinase-2
  • AI642394
  • fi09d05
  • fi17h06
  • wu:fi09d05
  • wu:fi17h06
  • wu:fi31b04
  • zgc:55926
  • ARABIDOPSIS THALIANA HEXOKINASE 2
  • ATHXK2
  • F6F22.11
  • F6F22_11
  • HEXOKINASE 2
  • hexokinase 2
  • hexokinase 2
  • hexokinase-2-like
  • similar to D.occidentalis hexokinase HXK which is able to phosphorylate glucose and fructose; similar to S. cerevisiae HXK2 (YGL253W)
  • HK2
  • hk2
  • Hk2
  • HXK2
  • LOC100856448
Epitope
N-Term
29
27
17
7
5
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
160
49
43
7
6
5
4
4
4
3
2
2
2
1
1
Host
Rabbit
113
60
Clonality
Polyclonal
Conjugate
This Hexokinase 2 antibody is un-conjugated
8
8
6
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
152
100
77
17
17
14
11
5
4
4
3
1
Supplier
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Catalog No. ABIN360640
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN360641 EIA IHC (p) WB Rabbit Ig AA 409-438, Middle Region Log in to see Polyclonal 5
1 ABIN263918 EIA WB Mouse IgG1 AA 1-917, N-Term Log in to see 1A7 2
1 ABIN1107525 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 3D3 3

Top referenced anti-Hexokinase 2 antibody for Enzyme Immunoassay

Similar anti-Hexokinase 2 Antibodies

Application / Reactivity Human
ELISA 92 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 73 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 141 Antibodies

General

Antigen Hexokinase 2 (HK2) Antibodies
Epitope N-Term
(29), (27), (17), (7), (5), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(160), (49), (43), (7), (6), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(113), (60)
Clonality
Polyclonal
Conjugate This Hexokinase 2 antibody is un-conjugated
(8), (8), (6), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(152), (100), (77), (17), (17), (14), (11), (5), (4), (4), (3), (1)
Pubmed 5 references available
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Product Details anti-Hexokinase 2 Antibody

Target Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN360640) Images
Specificity This antibody reacts to HK2.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human HK2.
Isotype Ig

Target Details Hexokinase 2

Product Details anti-Hexokinase 2 Antibody Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN360640) Images back to top
Antigen
Alternative Name Hexokinase-2 (HK2 Antibody Abstract)
Background In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.Synonyms: HK2, HKII, Hexokinase type II, Muscle form hexokinase
Gene ID 3099, 9606
UniProt P52789
Pathways PI3K-Akt Signaling, Carbohydrate Homeostasis

Application Details

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Handling References for anti-Hexokinase 2 Antibody (ABIN360640) Images back to top
Application Notes ELISA: 1: 1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details References for anti-Hexokinase 2 Antibody (ABIN360640) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Hexokinase 2 Antibody (ABIN360640)

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling Images back to top
Background publications

Kiritsy, Antoniades, Carlson, Beaulieu, DAndrea, Lynch et al.: "Combination of platelet-derived growth factor-BB and insulin-like growth factor-I is more effective than platelet-derived growth factor-BB alone in stimulating complete healing of full-thickness..." in: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 3, Issue 3, pp. 340-50, 2006

Kuwabara, Ishikawa, Kobayashi, Kobayashi, Sugiyama: "Renal clearance of a recombinant granulocyte colony-stimulating factor, nartograstim, in rats." in: Pharmaceutical research, Vol. 12, Issue 10, pp. 1466-9, 1996

Laakso, Malkki, Deeb: "Amino acid substitutions in hexokinase II among patients with NIDDM." in: Diabetes, Vol. 44, Issue 3, pp. 330-4, 1995

Echwald, Bjørbaek, Hansen, Clausen, Vestergaard, Zierath, Printz, Granner, Pedersen: "Identification of four amino acid substitutions in hexokinase II and studies of relationships to NIDDM, glucose effectiveness, and insulin sensitivity." in: Diabetes, Vol. 44, Issue 3, pp. 347-53, 1995

Shinohara, Yamamoto, Kogure, Ichihara, Terada: "Steady state transcript levels of the type II hexokinase and type 1 glucose transporter in human tumor cell lines." in: Cancer letters, Vol. 82, Issue 1, pp. 27-32, 1994

Images

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Supplier Images
 image for anti-Hexokinase 2 antibody (HK2) (N-Term) (ABIN360640) anti-Hexokinase 2 (HK2) (N-Term) antibody
 image for anti-Hexokinase 2 antibody (HK2) (N-Term) (ABIN360640) anti-Hexokinase 2 (HK2) (N-Term) antibody (Image 2)