anti-Human Hexokinase 2 antibody for Enzyme Immunoassay

Recommended Hexokinase 2 Antibody (supplied by: Log in to see )

Antigen
Hexokinase 2 (HK2) Antibodies
  • HK2
  • HKII
  • HXK2
  • hexokinase-2
  • AI642394
  • fi09d05
  • fi17h06
  • wu:fi09d05
  • wu:fi17h06
  • wu:fi31b04
  • zgc:55926
  • ARABIDOPSIS THALIANA HEXOKINASE 2
  • ATHXK2
  • F6F22.11
  • F6F22_11
  • HEXOKINASE 2
  • hexokinase 2
  • hexokinase 2
  • hexokinase-2-like
  • similar to D.occidentalis hexokinase HXK which is able to phosphorylate glucose and fructose; similar to S. cerevisiae HXK2 (YGL253W)
  • HK2
  • hk2
  • Hk2
  • HXK2
  • LOC100856448
Epitope
AA 1-917, N-Term
31
30
17
7
5
5
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
171
48
42
7
6
5
4
4
4
3
2
2
2
1
1
Host
Mouse
121
63
Clonality
Monoclonal
Conjugate
This Hexokinase 2 antibody is un-conjugated
9
8
6
6
5
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
158
101
80
22
22
14
13
8
4
4
4
1
1
Supplier
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Catalog No. ABIN263918
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN360641 EIA IHC (p) WB Rabbit Ig AA 409-438, Middle Region Log in to see Polyclonal 5
1 ABIN5552306 EIA IHC (p) WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
1 ABIN1107525 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 3D3 3
1 ABIN5565591 EIA RIA Rabbit AA 1-917 Log in to see Polyclonal

General

Antigen Hexokinase 2 (HK2) Antibodies
Epitope AA 1-917, N-Term
(31), (30), (17), (7), (5), (5), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(171), (48), (42), (7), (6), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1)
Host Mouse
(121), (63)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Hexokinase 2 antibody is un-conjugated
(9), (8), (6), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(158), (101), (80), (22), (22), (14), (13), (8), (4), (4), (4), (1), (1)
Pubmed 2 references available
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Product Details anti-Hexokinase 2 Antibody

Target Details Hexokinase 2 Application Details Handling ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) Images
Specificity This antibody detects Hexokinase 1, 2.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: Hexokinase type II, Muscle form hexokinase, HK2, HKII
Purification Protein-G affinity chromatography
Immunogen Recombinant human Hexokinase 2 (1-917aa) purified from E. coli
Clone 1A7
Isotype IgG1

Target Details Hexokinase 2

Product Details anti-Hexokinase 2 Antibody Application Details Handling ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) Images back to top
Antigen
Alternative Name Hexokinase-2 (HK2 Antibody Abstract)
Background Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.Synonyms: HK2, HKII, Hexokinase type II, Muscle form hexokinase
Gene ID 3099
UniProt P52789
Pathways PI3K-Akt Signaling, Carbohydrate Homeostasis

Application Details

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Handling ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) Images back to top
Application Notes ELISA. Western blot: 1: 1,000 approx. 1: 3,000, 1: 2,000 as starting dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate-Buffered Saline ( pH 7.4) with 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918)

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling Images back to top
Background publications

Wilson: "Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function." in: The Journal of experimental biology, Vol. 206, Issue Pt 12, pp. 2049-57, 2003

Furuta, Nishi, Le Beau, Fernald, Yano, Bell: "Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization." in: Genomics, Vol. 36, Issue 1, pp. 206-9, 1997

Images

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) back to top
Supplier Images
Western Blotting (WB) image for anti-Hexokinase 2 (HK2) (AA 1-917), (N-Term) antibody (ABIN263918) anti-Hexokinase 2 (HK2) (AA 1-917), (N-Term) antibody
Western Blotting (WB) image for anti-Hexokinase 2 (HK2) (AA 1-917), (N-Term) antibody (ABIN263918) anti-Hexokinase 2 (HK2) (AA 1-917), (N-Term) antibody (Image 2)