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anti-Human Hexokinase 2 antibody for Enzyme Immunoassay

Recommended Hexokinase 2 Antibody (supplied by: Log in to see )

Antigen
Hexokinase 2 (HK2) Antibodies
  • AI642394
  • ARABIDOPSIS THALIANA HEXOKINASE 2
  • ATHXK2
  • F6F22.11
  • F6F22_11
  • fi09d05
  • fi17h06
  • hexokinase-2
  • hexokinase 2
  • HEXOKINASE 2
  • HK2
  • HKII
  • HXK2
  • wu:fi09d05
  • wu:fi17h06
  • wu:fi31b04
  • zgc:55926
Epitope
AA 1-917, N-Term
32
25
16
9
5
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
155
37
31
3
3
2
1
1
1
1
Host
Mouse
112
54
Clonality
Monoclonal
Conjugate
This Hexokinase 2 antibody is un-conjugated
8
8
6
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
139
97
69
21
16
11
11
6
3
3
1
1
Supplier
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Catalog No. ABIN263918
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN360640 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 5
0.0008867729 ABIN360641 EIA IHC (p) WB Rabbit Ig AA 409-438, Middle Region Log in to see Polyclonal 5
0.0008867729 ABIN1107525 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 3D3 3

Top referenced anti-Hexokinase 2 antibody for Enzyme Immunoassay

Similar anti-Hexokinase 2 Antibodies

Application / Reactivity Human
ELISA 90 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 66 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 134 Antibodies

General

Antigen Hexokinase 2 (HK2) Antibodies
Epitope AA 1-917, N-Term
(32), (25), (16), (9), (5), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(155), (37), (31), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(112), (54)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Hexokinase 2 antibody is un-conjugated
(8), (8), (6), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(139), (97), (69), (21), (16), (11), (11), (6), (3), (3), (1), (1)
Pubmed 2 references available
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Product Details anti-Hexokinase 2 Antibody

Target Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN263918) Images
Specificity This antibody detects Hexokinase 1, 2.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: Hexokinase type II, Muscle form hexokinase, HK2, HKII
Purification Protein-G affinity chromatography
Immunogen Recombinant human Hexokinase 2 (1-917aa) purified from E. coli
Clone 1A7
Isotype IgG1

Target Details Hexokinase 2

Product Details anti-Hexokinase 2 Antibody Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN263918) Images back to top
Antigen
Alternative Name Hexokinase-2 (HK2 Antibody Abstract)
Background Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.Synonyms: HK2, HKII, Hexokinase type II, Muscle form hexokinase
Gene ID 3099
UniProt P52789
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Handling References for anti-Hexokinase 2 Antibody (ABIN263918) Images back to top
Application Notes ELISA. Western blot: 1: 1,000 approx. 1: 3,000, 1: 2,000 as starting dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details References for anti-Hexokinase 2 Antibody (ABIN263918) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate-Buffered Saline ( pH 7.4) with 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-Hexokinase 2 Antibody (ABIN263918)

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling Images back to top
Background publications

Wilson: "Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function." in: The Journal of experimental biology, Vol. 206, Issue Pt 12, pp. 2049-57, 2003

Furuta, Nishi, Le Beau, Fernald, Yano, Bell: "Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization." in: Genomics, Vol. 36, Issue 1, pp. 206-9, 1997

Images

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN263918) back to top
Supplier Images
Western Blotting (WB) image for anti-Hexokinase 2 antibody (HK2) (AA 1-917) (ABIN263918) anti-Hexokinase 2 (HK2) (AA 1-917), (N-Term) antibody
Western Blotting (WB) image for anti-Hexokinase 2 antibody (HK2) (AA 1-917) (ABIN263918) anti-Hexokinase 2 (HK2) (AA 1-917), (N-Term) antibody (Image 2)