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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||14 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||62 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||112 Antibodies|
|Antigen||Hexokinase 2 (HK2) Antibodies|
|Conjugate||This Hexokinase 2 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-Hexokinase 2 AntibodyTarget Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN969196) Images
|Immunogen||Purified recombinant fragment of human HK2 expressed in E. coli.|
Target Details Hexokinase 2Product Details anti-Hexokinase 2 Antibody Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN969196) Images back to top
|Alternative Name||HK2 (HK2 Antibody Abstract)|
|Background||The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases .Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.|
|Molecular Weight||102 kDa|
Application DetailsProduct Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Handling References for anti-Hexokinase 2 Antibody (ABIN969196) Images back to top
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details References for anti-Hexokinase 2 Antibody (ABIN969196) Images back to top
|Buffer||Ascitic fluid containing 0.03 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 4 °C or at -20 °C for long term.|
References for anti-Hexokinase 2 Antibody (ABIN969196)Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling Images back to top
|Product cited in:||
He, Bi, Zheng et al.: "Real-time quantitative RT-PCR assessment of PIM-1 and hK2 mRNA expression in benign prostate hyperplasia and prostate cancer." in: Medical oncology (Northwood, London, England), Vol. 26, Issue 3, pp. 303-8, 2009 (PubMed).
Paudyal, Oriuchi, Paudyal et al.: "Expression of glucose transporters and hexokinase II in cholangiocellular carcinoma compared using [18F]-2-fluro-2-deoxy-D-glucose positron emission tomography." in: Cancer science, Vol. 99, Issue 2, pp. 260-6, 2008 (PubMed).
Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).
ImagesProduct Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN969196) back to top