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anti-Human Hexokinase 2 antibody for Flow Cytometry

Recommended Hexokinase 2 Antibody (supplied by: Log in to see )

Antigen
Hexokinase 2 (HK2) Antibodies
  • AI642394
  • ARABIDOPSIS THALIANA HEXOKINASE 2
  • ATHXK2
  • F6F22.11
  • F6F22_11
  • fi09d05
  • fi17h06
  • hexokinase-2
  • hexokinase 2
  • HEXOKINASE 2
  • HK2
  • HKII
  • HXK2
  • wu:fi09d05
  • wu:fi17h06
  • wu:fi31b04
  • zgc:55926
Reactivity
Human
155
37
31
3
3
2
1
1
1
1
Host
Mouse
112
54
Clonality
Monoclonal
Conjugate
This Hexokinase 2 antibody is un-conjugated
8
8
6
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
139
96
68
21
15
11
11
6
4
3
1
1
Supplier
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Catalog No. ABIN969196
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN1107525 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 3D3 3
0.30226976 ABIN1845333 FACS IHC ELISA WB Mouse IgG1 Log in to see
0.0010482287 ABIN2453132 FACS IF IHC WB Mouse IgG1 Log in to see 4C5
0.0010482287 ABIN1804283 FACS IHC (p) ELISA WB Mouse IgG1 Log in to see 3D3
0.0010482287 ABIN1498647 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4C5
0.0010482287 ABIN2893543 FACS WB Mouse N-Term Log in to see
0.0010482287 ABIN2282743 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B379
0.0010482287 ABIN2281188 FACS WB Mouse IgG1 N-Term Log in to see 13k65
0.0010482287 ABIN2143426 FACS IF IHC WB Mouse IgG1 Log in to see 4C5
0.0010482287 ABIN4317173 ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 3D3
0.0010482287 ABIN4317175 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4C5
0.0010482287 ABIN2143329 FACS WB Mouse IgG1 Log in to see M80539
0.0010482287 ABIN1884927 FACS WB Mouse AA 270-435, N-Term Log in to see
0.0010482287 ABIN1577022 FACS IHC ELISA WB Mouse Log in to see
0.0010482287 ABIN1576981 FACS ELISA WB Mouse Log in to see

Top referenced anti-Hexokinase 2 antibody for Flow Cytometry

Similar anti-Hexokinase 2 Antibodies

Application / Reactivity Human
ELISA 90 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 66 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 134 Antibodies

General

Antigen Hexokinase 2 (HK2) Antibodies
Reactivity Human
(155), (37), (31), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(112), (54)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Hexokinase 2 antibody is un-conjugated
(8), (8), (6), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(139), (96), (68), (21), (15), (11), (11), (6), (4), (3), (1), (1)
Pubmed 3 references available
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Product Details anti-Hexokinase 2 Antibody

Target Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN969196) Images
Purification Ascites
Immunogen Purified recombinant fragment of human HK2 expressed in E. coli.
Clone 3D3
Isotype IgG1

Target Details Hexokinase 2

Product Details anti-Hexokinase 2 Antibody Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN969196) Images back to top
Antigen
Alternative Name HK2 (HK2 Antibody Abstract)
Background The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases .Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Molecular Weight 102 kDa
Gene ID 3099
HGNC 3099
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Handling References for anti-Hexokinase 2 Antibody (ABIN969196) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details References for anti-Hexokinase 2 Antibody (ABIN969196) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-Hexokinase 2 Antibody (ABIN969196)

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling Images back to top
Product cited in:

He, Bi, Zheng, Dai, Han, Liang, Ye, Zeng, Zhu, Zhong: "Real-time quantitative RT-PCR assessment of PIM-1 and hK2 mRNA expression in benign prostate hyperplasia and prostate cancer." in: Medical oncology (Northwood, London, England), Vol. 26, Issue 3, pp. 303-8, 2009

Paudyal, Oriuchi, Paudyal, Higuchi, Nakajima, Endo: "Expression of glucose transporters and hexokinase II in cholangiocellular carcinoma compared using [18F]-2-fluro-2-deoxy-D-glucose positron emission tomography." in: Cancer science, Vol. 99, Issue 2, pp. 260-6, 2008

Lim, Hao, Shaw, Patel, Szabó, Rual, Fisk, Li, Smolyar, Hill, Barabási, Vidal, Zoghbi: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006

Images

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN969196) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Hexokinase 2 antibody (HK2) (ABIN969196) Figure 1: Flow cytometric analysis of K562 cells using HK2 mouse mAb (green) and nega...
Immunohistochemistry (IHC) image for anti-Hexokinase 2 antibody (HK2) (ABIN969196) Figure 2: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues ...
Western Blotting (WB) image for anti-Hexokinase 2 antibody (HK2) (ABIN969196) Figure 3: Western blot analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and ...