anti-Human Hexokinase 2 antibody for Western Blotting

Recommended Hexokinase 2 Antibody (supplied by: Log in to see )

Antigen
Hexokinase 2 (HK2) Antibodies
  • HK2
  • HKII
  • HXK2
  • hexokinase-2
  • AI642394
  • fi09d05
  • fi17h06
  • wu:fi09d05
  • wu:fi17h06
  • wu:fi31b04
  • zgc:55926
  • ARABIDOPSIS THALIANA HEXOKINASE 2
  • ATHXK2
  • F6F22.11
  • F6F22_11
  • HEXOKINASE 2
  • hexokinase 2
  • hexokinase 2
  • hexokinase-2-like
  • similar to D.occidentalis hexokinase HXK which is able to phosphorylate glucose and fructose; similar to S. cerevisiae HXK2 (YGL253W)
  • HK2
  • hk2
  • Hk2
  • HXK2
  • LOC100856448
Epitope
AA 1-917, N-Term
31
30
17
7
5
5
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
171
49
43
7
6
5
4
4
4
3
2
2
2
1
1
Host
Mouse
122
62
Clonality
Monoclonal
Conjugate
This Hexokinase 2 antibody is un-conjugated
9
8
6
6
5
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
158
100
80
21
21
14
14
8
4
4
4
1
1
Supplier
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Catalog No. ABIN263918
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1455724 ABIN392755 IHC (p) WB Rabbit Ig AA 91-121, N-Term Log in to see Polyclonal 3
3.1455724 ABIN392756 IHC (p) WB Rabbit Ig AA 401-431, Center Log in to see Polyclonal 2
3.1455724 ABIN2785643 WB Rabbit Middle Region Log in to see Polyclonal
3.1455724 ABIN1882090 WB Rabbit AA 453-483, Center Log in to see Polyclonal 5
3.1455724 ABIN969196 FACS IHC ELISA WB Mouse IgG1 Log in to see 3D3 3
3.1455724 ABIN3021256 IHC WB Rabbit IgG Log in to see Polyclonal 1
3.1455724 ABIN306439 ELISA WB Mouse IgG1, kappa chain Log in to see 1A7 1
3.1455724 ABIN316081 ELISA WB Mouse IgG1 AA 1-917 Log in to see 1A7
3.1455724 ABIN516457 ELISA WB Mouse IgG2a kappa AA 818-917, partial Log in to see 4H1 1
3.1455724 ABIN1107525 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 3D3 3
3.1455724 ABIN2453131 WB Mouse IgG2b Log in to see 4D4
3.1455724 ABIN516456 WB Mouse AA 1-917, full length Log in to see Polyclonal 1
3.1455724 ABIN2459729 ELISA WB Rabbit Log in to see Polyclonal
3.1455724 ABIN4317176 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.1455724 ABIN1873023 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.1455724 ABIN4317175 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4C5
3.1455724 ABIN4317173 ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 3D3
3.1455724 ABIN1859176 ICC IHC IP WB Rabbit IgG AA 619-917 Log in to see Polyclonal
3.1455724 ABIN2966709 IC IF WB Rabbit Log in to see Polyclonal
3.1455724 ABIN634132 WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-Hexokinase 2 antibody for Western Blotting

Similar anti-Hexokinase 2 Antibodies

Application / Reactivity Human
ELISA 92 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 76 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 147 Antibodies

General

Antigen Hexokinase 2 (HK2) Antibodies
Epitope AA 1-917, N-Term
(31), (30), (17), (7), (5), (5), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(171), (49), (43), (7), (6), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1)
Host Mouse
(122), (62)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Hexokinase 2 antibody is un-conjugated
(9), (8), (6), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(158), (100), (80), (21), (21), (14), (14), (8), (4), (4), (4), (1), (1)
Pubmed 2 references available
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Product Details anti-Hexokinase 2 Antibody

Target Details Hexokinase 2 Application Details Handling ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) Images
Specificity This antibody detects Hexokinase 1, 2.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: Hexokinase type II, Muscle form hexokinase, HK2, HKII
Purification Protein-G affinity chromatography
Immunogen Recombinant human Hexokinase 2 (1-917aa) purified from E. coli
Clone 1A7
Isotype IgG1

Target Details Hexokinase 2

Product Details anti-Hexokinase 2 Antibody Application Details Handling ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) Images back to top
Antigen
Alternative Name Hexokinase-2 (HK2 Antibody Abstract)
Background Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.Synonyms: HK2, HKII, Hexokinase type II, Muscle form hexokinase
Gene ID 3099
UniProt P52789
Pathways PI3K-Akt Signaling, Carbohydrate Homeostasis

Application Details

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Handling ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) Images back to top
Application Notes ELISA. Western blot: 1: 1,000 approx. 1: 3,000, 1: 2,000 as starting dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate-Buffered Saline ( pH 7.4) with 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918)

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling Images back to top
Background publications

Wilson: "Isozymes of mammalian hexokinase: structure, subcellular localization and metabolic function." in: The Journal of experimental biology, Vol. 206, Issue Pt 12, pp. 2049-57, 2003

Furuta, Nishi, Le Beau, Fernald, Yano, Bell: "Sequence of human hexokinase III cDNA and assignment of the human hexokinase III gene (HK3) to chromosome band 5q35.2 by fluorescence in situ hybridization." in: Genomics, Vol. 36, Issue 1, pp. 206-9, 1997

Images

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling ProductDetails: References for anti-Hexokinase 2 antibody (ABIN263918) back to top
Supplier Images
Western Blotting (WB) image for anti-Hexokinase 2 antibody (HK2) (AA 1-917) (ABIN263918) anti-Hexokinase 2 (HK2) (AA 1-917), (N-Term) antibody
Western Blotting (WB) image for anti-Hexokinase 2 antibody (HK2) (AA 1-917) (ABIN263918) anti-Hexokinase 2 (HK2) (AA 1-917), (N-Term) antibody (Image 2)