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anti-Human Hexokinase 2 antibody for Western Blotting

Recommended Hexokinase 2 Antibody (supplied by: Log in to see )

Antigen
Hexokinase 2 (HK2) Antibodies
  • HK2
  • HKII
  • HXK2
  • hexokinase-2
  • AI642394
  • fi09d05
  • fi17h06
  • wu:fi09d05
  • wu:fi17h06
  • wu:fi31b04
  • zgc:55926
  • ARABIDOPSIS THALIANA HEXOKINASE 2
  • ATHXK2
  • F6F22.11
  • F6F22_11
  • HEXOKINASE 2
  • hexokinase 2
  • hexokinase 2
  • hexokinase-2-like
  • similar to D.occidentalis hexokinase HXK which is able to phosphorylate glucose and fructose; similar to S. cerevisiae HXK2 (YGL253W)
  • HK2
  • hk2
  • Hk2
  • HXK2
  • LOC100856448
Epitope
AA 91-121, N-Term
29
27
17
7
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
160
49
43
7
6
5
4
4
4
3
2
2
2
1
1
Host
Rabbit
113
60
Clonality
Polyclonal
Conjugate
This Hexokinase 2 antibody is un-conjugated
8
8
6
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
152
100
77
17
17
14
11
5
4
4
4
1
Options
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Catalog No. ABIN392755
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0997953 ABIN263918 EIA WB Mouse IgG1 AA 1-917, N-Term Log in to see 1A7 2
3.0997953 ABIN392756 IHC (p) WB Rabbit Ig AA 401-431, Center Log in to see Polyclonal 2
3.0997953 ABIN969196 FACS IHC ELISA WB Mouse IgG1 Log in to see 3D3 3
3.0997953 ABIN1882090 WB Rabbit AA 453-483, Center Log in to see Polyclonal 5
3.0997953 ABIN3021256 IHC WB Rabbit IgG Log in to see Polyclonal 1
3.0997953 ABIN306439 ELISA WB Mouse IgG1, kappa chain Log in to see 1A7 1
3.0997953 ABIN1107525 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 3D3 3
3.0997953 ABIN316081 ELISA WB Mouse IgG1 AA 1-917 Log in to see 1A7
3.0997953 ABIN516457 ELISA WB Mouse IgG2a kappa AA 818-917, partial Log in to see 4H1 1
3.0997953 ABIN2453131 WB Mouse IgG2b Log in to see 4D4
3.0997953 ABIN516456 WB Mouse AA 1-917, full length Log in to see Polyclonal 1
3.0997953 ABIN1873023 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN2459729 ELISA WB Rabbit Log in to see Polyclonal
3.0997953 ABIN4317176 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.0997953 ABIN4317173 ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 3D3
3.0997953 ABIN4317175 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4C5
3.0997953 ABIN1859176 ICC IHC IP WB Rabbit IgG AA 619-917 Log in to see Polyclonal
3.0997953 ABIN2966709 IC IF WB Rabbit Log in to see Polyclonal
3.0997953 ABIN634132 WB Rabbit N-Term Log in to see Polyclonal
3.0997953 ABIN632021 WB Rabbit Middle Region Log in to see Polyclonal

Top referenced anti-Hexokinase 2 antibody for Western Blotting

Similar anti-Hexokinase 2 Antibodies

Application / Reactivity Human
ELISA 92 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 73 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 141 Antibodies

General

Antigen Hexokinase 2 (HK2) Antibodies
Epitope AA 91-121, N-Term
(29), (27), (17), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(160), (49), (43), (7), (6), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(113), (60)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Hexokinase 2 antibody is un-conjugated
(8), (8), (6), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(152), (100), (77), (17), (17), (14), (11), (5), (4), (4), (4), (1)
Pubmed 3 references available
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Product Details anti-Hexokinase 2 Antibody

Target Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN392755) Images
Specificity This HK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99~128 amino acids from the N-terminal region of human HK2.
Predicted Reactivity Mouse (Murine),Pig (Porcine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This HK2 (Hexokinase II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-121 AA from the N-terminal region of human HK2 (Hexokinase II).
Clone RB3917
Isotype Ig

Target Details Hexokinase 2

Product Details anti-Hexokinase 2 Antibody Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN392755) Images back to top
Antigen
Alternative Name HK2 (Hexokinase II) (HK2 Antibody Abstract)
Background In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.
Synonyms: Hexokinase, type II, HK II, Muscle form hexokinase
Molecular Weight 102367 DA
Gene ID 3099
UniProt P52789
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure
Pathways PI3K-Akt Signaling, Carbohydrate Homeostasis

Application Details

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Handling References for anti-Hexokinase 2 Antibody (ABIN392755) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details References for anti-Hexokinase 2 Antibody (ABIN392755) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-Hexokinase 2 Antibody (ABIN392755)

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling Images back to top
Product cited in:

Palmieri, Fitzgerald, Shreeve, Hua, Bronder, Weil, Davis, Stark, Merino, Kurek, Mehdorn, Davis, Steinberg, Meltzer, Aldape, Steeg: "Analyses of resected human brain metastases of breast cancer reveal the association between up-regulation of hexokinase 2 and poor prognosis." in: Molecular cancer research : MCR, Vol. 7, Issue 9, pp. 1438-45, 2009

Background publications

Laakso, Malkki, Deeb: "Amino acid substitutions in hexokinase II among patients with NIDDM." in: Diabetes, Vol. 44, Issue 3, pp. 330-4, 1995

Echwald, Bjørbaek, Hansen, Clausen, Vestergaard, Zierath, Printz, Granner, Pedersen: "Identification of four amino acid substitutions in hexokinase II and studies of relationships to NIDDM, glucose effectiveness, and insulin sensitivity." in: Diabetes, Vol. 44, Issue 3, pp. 347-53, 1995

Images

Product Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling References for anti-Hexokinase 2 Antibody (ABIN392755) back to top
Supplier Images
Western Blotting (WB) image for anti-Hexokinase 2 antibody (HK2) (AA 91-121) (ABIN392755) The anti-HK2 Pab (ABIN392755) is used in Western blot to detect HK2 in A375 cell lysate.
Immunohistochemistry (IHC) image for anti-Hexokinase 2 antibody (HK2) (AA 91-121) (ABIN392755) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 an...