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|Antigen||Hexokinase 2 (HK2) Antibodies|
|Epitope||AA 74-123 Alternatives|
|Reactivity||Bat, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish Alternatives|
|Conjugate||This Hexokinase 2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Hexokinase 2 AntibodyTarget Details Hexokinase 2 Application Details Handling Images
|Specificity||Human HK2 / Hexokinase 2|
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Chicken, Zebrafish (85%).|
Synthetic peptide located between aa74-123 of human HK2 (P52789, NP_000180). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Sablefish, Stickleback (92%), Orangutan, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish, Sea squirt (85%).
Type of Immunogen: Synthetic peptide
Target Details Hexokinase 2Product Details anti-Hexokinase 2 Antibody Application Details Handling Images back to top
|Alternative Name||HK2 / Hexokinase 2 (HK2 Antibody Abstract)|
Name/Gene ID: HK2
Family: Non-protein Kinase
Synonyms: HK2, Hexokinase II, Hexokinase type II, Hexokinase-2, muscle, HK II, HXK2, Hexokinase-2, Muscle form hexokinase, Hexokinase 2, HKII, Hxk 2
|Pathways||PI3K-Akt Signaling, Carbohydrate Homeostasis|
Application DetailsProduct Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Handling Images back to top
Approved: IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Images back to top
|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
ImagesProduct Details anti-Hexokinase 2 Antibody Target Details Hexokinase 2 Application Details Handling back to top