anti-Human Huntingtin antibody for Immunohistochemistry

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • HTT
  • hd
  • ZHD
  • CG9995
  • Dmel\\CG9995
  • HD
  • Hsap\\HD
  • Htt
  • dHtt
  • dhtt
  • SLC6A4
  • huntington
  • it15
  • htt
  • IT15
  • AI256365
  • C430023I11Rik
  • Hd
  • Hdh
  • solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
  • huntingtin
  • SLC6A4
  • LOC100216476
  • htt
  • Htt
  • HTT
  • HDH
Epitope
AA 416-424, pSer421
14
12
10
8
8
7
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
154
103
62
13
6
2
2
2
1
1
1
Host
Rabbit
81
78
3
1
Clonality
Polyclonal
Conjugate
This Huntingtin antibody is un-conjugated
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
133
75
73
37
26
25
24
17
13
8
4
3
2
1
Options
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Catalog No. ABIN100604
$ 379.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.976315 ABIN2470221 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
8.976315 ABIN2736926 IHC WB Rabbit IgG Log in to see Polyclonal
8.976315 ABIN2471198 IHC ELISA WB Mouse IgG2a kappa Log in to see 3F1
1 ABIN1450584 IHC ELISA Rabbit IgG AA 387-436 Log in to see Polyclonal 3
1 ABIN269392 IHC (fro) IP IHC WB Mouse IgG1 AA 2703-2911 Log in to see HDC8A4
1 ABIN269391 IHC (fro) IP IHC WB Mouse IgG1 Log in to see HDB4E10
1 ABIN4320700 ELISA ICC IF IHC WB Rabbit C-Term Log in to see Polyclonal 3
1 ABIN153591 IHC (fro) IP IHC WB Mouse IgG1 Log in to see HDA3E10
1 ABIN252646 IHC IHC (fro) WB Mouse IgG Log in to see 1A771
1 ABIN1450323 IHC ELISA Rabbit IgG AA 387-436, pSer421 Log in to see Polyclonal
1 ABIN4320703 IF IHC IHC (p) WB Mouse IgG Log in to see 4-13
1 ABIN4320702 ELISA IF IHC IHC (p) WB Mouse IgG Log in to see 3-19
1 ABIN3185117 ELISA IHC Rabbit IgG Ser47 Log in to see Polyclonal
1 ABIN3182875 ELISA IHC Rabbit IgG pSer421 Log in to see Polyclonal
1 ABIN4320701 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN4320699 ELISA ICC IF IHC WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1607890 FM IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1607891 FM IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN790734 IHC WB Mouse IgG N-Term Log in to see H-F5
1 ABIN2741589 IF IHC WB Mouse IgG AA 544-552 Log in to see 4-13

General

Antigen Huntingtin (HTT) Antibodies
Epitope AA 416-424, pSer421
(14), (12), (10), (8), (8), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(154), (103), (62), (13), (6), (2), (2), (2), (1), (1), (1)
Host Rabbit
(81), (78), (3), (1)
Clonality
Polyclonal
Conjugate This Huntingtin antibody is un-conjugated
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(133), (75), (73), (37), (26), (25), (24), (17), (13), (8), (4), (3), (2), (1)
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling Images
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 416-424 of Human huntingtin protein.
Isotype IgG

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Huntington (HTT Antibody Abstract)
Background Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons.  It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization.  This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
Synonyms: AI256365 antibody, C430023I11Rik antibody, HD antibody, HD protein antibody, HDH antibody, HTT antibody, HUNTINGTON CHOREA antibody, Huntington disease protein antibody, Huntingtin protein antibody, IT15,
Gene ID 3064
UniProt P42858
Pathways PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling Images back to top
Application Notes Anti-Huntingtin pS421 antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect bands at approximately 350 kDa and 200 kDa in size corresponding to full-length Huntingtin protein and truncated (hypothetical) Huntingtin protein, respectively, by western blotting in the appropriate cell lysate or extract.   This antibody is specific for the phosphorylated form of Huntingtin protein at the pS421 residue.  The identity of lower molecular bands ~130 kDa is not known.
Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Images back to top
Format Liquid
Concentration 0.6 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

Images

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Huntingtin (HTT) (AA 416-424), (pSer421) antibody (ABIN100604) Affinity Purified anti-Huntingtin pS421 antibody was used at a 1:100 dilution to dete...
Western Blotting (WB) image for anti-Huntingtin (HTT) (AA 416-424), (pSer421) antibody (ABIN100604) anti-Huntingtin (HTT) (AA 416-424), (pSer421) antibody (Image 2)
Western Blotting (WB) image for anti-Huntingtin (HTT) (AA 416-424), (pSer421) antibody (ABIN100604) anti-Huntingtin (HTT) (AA 416-424), (pSer421) antibody (Image 3)