anti-Human Huntingtin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • HTT
  • hd
  • ZHD
  • CG9995
  • Dmel\\CG9995
  • HD
  • Hsap\\HD
  • Htt
  • dHtt
  • dhtt
  • SLC6A4
  • huntington
  • it15
  • htt
  • IT15
  • AI256365
  • C430023I11Rik
  • Hd
  • Hdh
  • solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
  • huntingtin
  • SLC6A4
  • LOC100216476
  • htt
  • Htt
  • HTT
  • HDH
Epitope
pSer421
14
12
10
8
8
7
5
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
154
103
62
13
6
2
2
2
1
1
1
Host
Rabbit
81
78
3
1
Clonality
Polyclonal
Conjugate
This Huntingtin antibody is un-conjugated
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
133
75
74
37
25
25
24
17
13
8
4
3
2
1
Supplier
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Catalog No. ABIN214338
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.671195 ABIN4320701 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN303085 EIA IHC (p) WB Rabbit AA 416-424, pSer421 Log in to see Polyclonal
1 ABIN781873 EIA IHC (p) WB Mouse IgG2a AA 81-191 Log in to see 3F1
1 ABIN1387692 IF (p) IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN626061 IHC (p) ELISA WB Mouse IgG2a, kappa AA 81-191 Log in to see 3F1
1 ABIN790678 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 2703-2911 Log in to see
1 ABIN4320703 IF IHC IHC (p) WB Mouse IgG Log in to see 4-13
1 ABIN4320702 ELISA IF IHC IHC (p) WB Mouse IgG Log in to see 3-19
1 ABIN1410710 IHC (p) HRP Rabbit IgG Log in to see Polyclonal
1 ABIN1392817 IHC (p) Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1735629 IHC (p) ELISA WB Rabbit IgG pSer421 Log in to see Polyclonal
1 ABIN516382 IHC (p) ELISA WB Mouse IgG2a kappa AA 81-190, partial Log in to see 3F1
1 ABIN2622231 IHC (fro) IF IHC (p) WB Goat IgG AA 85-200 Log in to see Polyclonal
1 ABIN1723674 IHC (p) ELISA WB Mouse IgG2a kappa AA 81-191 Log in to see 3F1
1 ABIN228545 ICC IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 AA 181-810 Log in to see 2Q75
1 ABIN228544 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG1 AA 2146-2541 Log in to see
1 ABIN228543 ICC IHC (fro) IHC (p) IP ELISA WB Mouse IgG2b AA 1247-1646 Log in to see
1 ABIN2622232 ELISA IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN4320704 ELISA ICC IF IHC (p) WB Mouse IgG1 Log in to see HU-2E8
1 ABIN1868465 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal

General

Antigen Huntingtin (HTT) Antibodies
Epitope pSer421
(14), (12), (10), (8), (8), (7), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(154), (103), (62), (13), (6), (2), (2), (2), (1), (1), (1)
Host Rabbit
(81), (78), (3), (1)
Clonality
Polyclonal
Conjugate This Huntingtin antibody is un-conjugated
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(133), (75), (74), (37), (25), (25), (24), (17), (13), (8), (4), (3), (2), (1)
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling Images
Brand IHC-plus™
Specificity Amino acids 416 to 424 of Human huntingtin protein phosphorylated at Ser421
Purification Immunoaffinity purified
Immunogen Synthetic peptide from human HTT / Huntingtin.

Type of Immunogen: Synthetic peptide

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling Images back to top
Antigen
Alternative Name HTT / Huntingtin (HTT Antibody Abstract)
Background Name/Gene ID: HTT
Family: Hungtintin

Synonyms: HTT, Huntingtin, Huntington disease protein, IT15, HD, HD protein
Gene ID 3064
UniProt P42858
Pathways PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling Images back to top
Application Notes Approved: ELISA (1:10000 - 1:40000), IHC-P (10 - 20 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10-20 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Huntingtin (HTT) (pSer421) antibody (ABIN214338) Human Brain Cerebellum (formalin-fixed, paraffin-embedded) stained with HTT antibody ...