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anti-Mouse (Murine) Huntingtin antibody for Western Blotting

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • AI256365
  • C430023I11Rik
  • CG9995
  • dHtt
  • dhtt
  • Dmel\\CG9995
  • HD
  • hd
  • Hd
  • Hdh
  • Hsap\\HD
  • htt
  • HTT
  • Htt
  • huntington
  • it15
  • IT15
  • SLC6A4
  • ZHD
Epitope
AA 997-1276
14
13
12
9
7
7
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rabbit
152
72
42
6
2
Host
Mouse
81
74
3
1
Clonality
Monoclonal
Conjugate
This Huntingtin antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
124
74
49
24
20
18
18
18
11
8
3
3
2
Supplier
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Catalog No. ABIN119305
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13383192 ABIN119307 IHC (fro) IP WB Mouse IgG1 AA 2703-2911 Log in to see HDC8A4 1
0.13383192 ABIN119306 IHC (fro) IP WB Mouse IgG1 AA 1844-2131 Log in to see HDB4E10 2
0.13383192 ABIN269391 IHC (fro) IP IHC WB Mouse IgG1 AA 1844-2131 Log in to see HDB4E10
0.13383192 ABIN153591 IHC (fro) IP IHC WB Mouse IgG1 AA 997-1276 Log in to see HDA3E10
0.13383192 ABIN1868466 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 782-920 Log in to see Polyclonal
0.13383192 ABIN2474102 IHC (fro) IP WB Mouse IgG1 Log in to see HDB4E10 4
0.13383192 ABIN2474103 IHC (fro) IP WB Mouse IgG1 Log in to see HDC8A4 4
0.13383192 ABIN2474101 IHC (fro) IP WB Mouse IgG2a Log in to see HDA3E10 1
0.0011757269 ABIN303085 EIA IHC (p) WB Rabbit AA 416-424, pSer421 Log in to see Polyclonal
0.0011757269 ABIN350376 IHC WB Rabbit AA 1150-1200 Log in to see Polyclonal 2
0.0011757269 ABIN1842290 IHC WB Mouse IgG1 AA 1-225 Log in to see 3
0.0011757269 ABIN315900 ICC IHC (fro) IF IHC (p) IHC WB Rabbit Internal Region Log in to see Polyclonal
0.0011757269 ABIN1735629 IHC (p) ELISA WB Rabbit IgG pSer421 Log in to see Polyclonal
0.0011757269 ABIN350377 IHC WB Rabbit IgG AA 1150-1200 Log in to see Polyclonal 2
0.0011757269 ABIN1607890 FM IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011757269 ABIN1607891 FM IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011757269 ABIN2736926 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011757269 ABIN1514987 IHC ELISA WB Rabbit IgG N-Term, pSer13 Log in to see Polyclonal
0.0011757269 ABIN1514988 ICC ELISA WB Rabbit IgG N-Term, pSer17 Log in to see Polyclonal
0.0011757269 ABIN1514986 ICC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-Huntingtin antibody for Western Blotting

Similar anti-Huntingtin Antibodies

Application / Reactivity Rabbit Mouse (Murine) Human
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies 13 Antibodies
Immunoprecipitation (IP) 7 Antibodies 12 Antibodies
Western Blotting (WB) 7 Antibodies 57 Antibodies
ELISA 26 Antibodies 72 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Dot Blot (DB) 3 Antibodies

General

Antigen Huntingtin (HTT) Antibodies
Epitope AA 997-1276
(14), (13), (12), (9), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rabbit
(152), (72), (42), (6), (2)
Host Mouse
(81), (74), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Huntingtin antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(124), (74), (49), (24), (20), (18), (18), (18), (11), (8), (3), (3), (2)
Pubmed 2 references available
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN119305) Images
Specificity This antibody reacts with an epitope corresponding to the HDA region (amino acids 1171 - 1177) of the huntingtin protein. Clone HDA3E10 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. The combined use of clone HDA3E10 (SM1660) HDC8A4 (SM1662) and HDB4E10 (SM1661) demonstrate that huntingtin is enriched in neuronal cells in the brain.
Purification Purified
Immunogen Recombinant protein corresponding to amino acids 997 -1276 of huntingtin
Clone HDA3E10
Isotype IgG2a

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling References for anti-Huntingtin Antibody (ABIN119305) Images back to top
Antigen
Alternative Name Huntingtin (HTT Antibody Abstract)
Background Huntingtin is a 350 kDa protein of 3144 amino acids that is altered in Huntington's disease (HD). The expanded trinucleotide CAG repeat of the HD gene encodes an abnormally expanded polyglutamine stretch in the N-terminus of the Huntingtin protein. The normal function of Huntingtin still remains uncertain but it has been suggested that it performs a necessary housekeeping function. The abnormal form of Huntingtin aggregates in vitro and forms neuronal intranuclear and cytoplasmic inclusions in HD patients. The expanded polyglutamine repeats formed by this mutation of the Huntingtin gene have been proposed to cause neuronal degeneration in HD through abnormal interactions with other proteins containing short polyglutamine tracts such as the CREB binding protein (CBP), a protein that promotes cell survival and is a major mediator of survival signals in mature neurons.Synonyms: HD, HTT, Huntington Disease Protein, IT15
Gene ID 3064
NCBI Accession NP_002102
UniProt P42858
Research Area Neurology, Virology
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling References for anti-Huntingtin Antibody (ABIN119305) Images back to top
Application Notes Western blotting. Immunoprecipitation. Immunohistochemistry on frozen sections, recommended positive control tissue: Brain (Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol(1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details References for anti-Huntingtin Antibody (ABIN119305) Images back to top
Concentration 1.0 mg/mL
Buffer PBS buffer with 0.09 % sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Huntingtin Antibody (ABIN119305)

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling Images back to top
Background publications

Nucifora, Sasaki, Peters, Huang, Cooper, Yamada, Takahashi, Tsuji, Troncoso, Dawson, Dawson, Ross: "Interference by huntingtin and atrophin-1 with cbp-mediated transcription leading to cellular toxicity." in: Science (New York, N.Y.), Vol. 291, Issue 5512, pp. 2423-8, 2001

Wilkinson, Nguyen, Manilal, Thomas, Neal, Harper, Jones, Morris: "Localization of rabbit huntingtin using a new panel of monoclonal antibodies." in: Brain research. Molecular brain research, Vol. 69, Issue 1, pp. 10-20, 1999

Images

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN119305) back to top
Supplier Images
Western Blotting (WB) image for anti-Huntingtin antibody (HTT) (AA 997-1276) (ABIN119305) Total protein extract of normal human cerebral cortex separated as a strip on a 3-12....