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anti-Mouse (Murine) Huntingtin antibody for Western Blotting

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • AI256365
  • C430023I11Rik
  • CG9995
  • dHtt
  • dhtt
  • Dmel\\CG9995
  • HD
  • hd
  • Hd
  • Hdh
  • Hsap\\HD
  • htt
  • HTT
  • Htt
  • huntington
  • it15
  • IT15
  • SLC6A4
  • ZHD
Epitope
AA 2703-2911
13
12
12
9
7
7
6
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rabbit
151
76
43
9
2
Host
Mouse
80
74
3
1
Clonality
Monoclonal
Conjugate
This Huntingtin antibody is un-conjugated
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
123
72
52
24
23
22
18
18
12
8
3
3
2
Supplier
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Catalog No. ABIN119307
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN119305 IHC (fro) IP WB Mouse IgG2a AA 997-1276 Log in to see HDA3E10 2
0.124960825 ABIN119306 IHC (fro) IP WB Mouse IgG1 AA 1844-2131 Log in to see HDB4E10 2
0.124960825 ABIN252646 IHC IHC (fro) WB Mouse IgG Log in to see 1A771
0.124960825 ABIN269391 IHC (fro) IP IHC WB Mouse IgG1 Log in to see HDB4E10
0.124960825 ABIN153591 IHC (fro) IP IHC WB Mouse IgG1 Log in to see HDA3E10
0.124960825 ABIN269392 IHC (fro) IP IHC WB Mouse IgG1 AA 2703-2911 Log in to see HDC8A4
0.124960825 ABIN1868466 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 782-920 Log in to see Polyclonal
0.124960825 ABIN4320700 ELISA ICC IF IHC WB Rabbit C-Term Log in to see Polyclonal 3
0.124960825 ABIN2474102 IHC (fro) IP WB Mouse IgG1 Log in to see HDB4E10 4
0.124960825 ABIN2474103 IHC (fro) IP WB Mouse IgG1 Log in to see HDC8A4 4
0.124960825 ABIN2474101 IHC (fro) IP WB Mouse IgG2a Log in to see HDA3E10 1
0.124960825 ABIN4320703 ICC IF IHC IHC (p) WB Mouse IgG Log in to see 41377
0.124960825 ABIN4320702 ELISA ICC IF IHC IHC (p) WB Mouse IgG Log in to see 41352
0.124960825 ABIN4320699 ELISA ICC IF IHC WB Rabbit N-Term Log in to see Polyclonal
0.124960825 ABIN3031257 WB Rabbit IgG C-Term Log in to see Polyclonal
0.0012207722 ABIN303085 EIA IHC (p) WB Rabbit AA 416-424, pSer421 Log in to see Polyclonal
0.0012207722 ABIN350376 IHC WB Rabbit AA 1150-1200 Log in to see Polyclonal 2
0.0012207722 ABIN1842290 IHC WB Mouse IgG1 AA 1-225 Log in to see 3
0.0012207722 ABIN350377 IHC WB Rabbit IgG AA 1150-1200 Log in to see Polyclonal 2
0.0012207722 ABIN1735629 IHC (p) ELISA WB Rabbit IgG pSer421 Log in to see Polyclonal

Top referenced anti-Huntingtin antibody for Western Blotting

Similar anti-Huntingtin Antibodies

Application / Reactivity Rabbit Mouse (Murine) Human
Immunohistochemistry (IHC) 3 Antibodies 25 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies 13 Antibodies
Immunoprecipitation (IP) 10 Antibodies 12 Antibodies
Western Blotting (WB) 10 Antibodies 56 Antibodies
ELISA 26 Antibodies 70 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Dot Blot (DB) 3 Antibodies

General

Antigen Huntingtin (HTT) Antibodies
Epitope AA 2703-2911
(13), (12), (12), (9), (7), (7), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rabbit
(151), (76), (43), (9), (2)
Host Mouse
(80), (74), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Huntingtin antibody is un-conjugated
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(123), (72), (52), (24), (23), (22), (18), (18), (12), (8), (3), (3), (2)
Pubmed 1 reference available
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN119307) Images
Specificity This antibody reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein. Clone HDC8A4 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. Clone HDC8A4 recognises both denatured and native huntingtin in human brain. The combined use of clone HDC8A4 (SM1662), HDB4E10 (SM1661) and HDA3E10 (SM1660) demonstrate that huntingtin is enriched in neuronal cells in the brain.
Purification Affinity chromatography on Protein G
Immunogen Recombinant protein corresponding to amino acids 2703 - 2911 of huntingtin
Clone HDC8A4
Isotype IgG1

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling References for anti-Huntingtin Antibody (ABIN119307) Images back to top
Antigen
Alternative Name Huntingtin (HTT Antibody Abstract)
Background Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegeneative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years. The HD gene product is widely expressed in human tissues, with the highest level of expression in the brain. The huntingtin gene product is expressed at similar levels in patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using yeast two-hybrid system, HAP1 (huntingtin associated protein 1) has been identified, that associates with huntingtin protein. The In vitro data suggest that the association between HAP1 and huntingtin is enhanced by increasing length of glutamine repeat.Synonyms: HD, HTT, Huntington Disease Protein, IT15
Gene ID 3064
NCBI Accession NP_002102
UniProt P42858
Research Area Neurology, Virology
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling References for anti-Huntingtin Antibody (ABIN119307) Images back to top
Application Notes Immunohistochemistry on frozen sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details References for anti-Huntingtin Antibody (ABIN119307) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Huntingtin Antibody (ABIN119307)

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling Images back to top
Background publications

Wilkinson, Nguyen, Manilal, Thomas, Neal, Harper, Jones, Morris: "Localization of rabbit huntingtin using a new panel of monoclonal antibodies." in: Brain research. Molecular brain research, Vol. 69, Issue 1, pp. 10-20, 1999

Images

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN119307) back to top
Supplier Images
Western Blotting (WB) image for anti-Huntingtin antibody (HTT) (AA 2703-2911) (ABIN119307) Total protein extract of normal human cerebral cortex separated as a strip on a 3-12....