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anti-Rabbit Huntingtin antibody for Immunohistochemistry (Frozen Sections)

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • HTT
  • hd
  • ZHD
  • CG9995
  • Dmel\\CG9995
  • HD
  • Hsap\\HD
  • Htt
  • dHtt
  • dhtt
  • SLC6A4
  • huntington
  • it15
  • htt
  • IT15
  • AI256365
  • C430023I11Rik
  • Hd
  • Hdh
  • solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
  • huntingtin
  • SLC6A4
  • LOC100216476
  • htt
  • Htt
  • HTT
  • HDH
Epitope
AA 2703-2911
14
12
12
8
7
5
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rabbit
148
98
58
12
6
2
2
2
1
1
1
Host
Mouse
79
74
3
1
Clonality
Monoclonal
Conjugate
This Huntingtin antibody is un-conjugated
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
128
72
71
36
26
24
23
17
13
8
4
3
2
1
Supplier
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Catalog No. ABIN119307
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN119305 IHC (fro) IP WB Mouse IgG2a AA 997-1276 Log in to see HDA3E10 2
1 ABIN119306 IHC (fro) IP WB Mouse IgG1 AA 1844-2131 Log in to see HDB4E10 2
1 ABIN269392 IHC (fro) IP IHC WB Mouse IgG1 AA 2703-2911 Log in to see HDC8A4
1 ABIN269391 IHC (fro) IP IHC WB Mouse IgG1 Log in to see HDB4E10
1 ABIN153591 IHC (fro) IP IHC WB Mouse IgG1 Log in to see HDA3E10
1 ABIN790678 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 2703-2911 Log in to see
1 ABIN2474101 IHC (fro) IP WB Mouse IgG2a Log in to see HDA3E10 1
1 ABIN2474102 IHC (fro) IP WB Mouse IgG1 Log in to see HDB4E10 4
1 ABIN2474103 IHC (fro) IP WB Mouse IgG1 Log in to see HDC8A4 4
1 ABIN201063 IHC (fro) IP WB Mouse IgG2a AA 1171-1177 Log in to see
1 ABIN201061 IHC (fro) IP WB Mouse IgG1 AA 1844-2131 Log in to see
1 ABIN644810 IHC (fro) IP WB Mouse IgG1 Log in to see

Top referenced anti-Huntingtin antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Huntingtin Antibodies

Application / Reactivity Rabbit Mouse (Murine) Human
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 13 Antibodies
Immunoprecipitation (IP) 13 Antibodies 15 Antibodies
Western Blotting (WB) 13 Antibodies 75 Antibodies
ELISA 37 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Dot Blot (DB) 3 Antibodies

General

Antigen Huntingtin (HTT) Antibodies
Epitope AA 2703-2911
(14), (12), (12), (8), (7), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rabbit
(148), (98), (58), (12), (6), (2), (2), (2), (1), (1), (1)
Host Mouse
(79), (74), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Huntingtin antibody is un-conjugated
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(128), (72), (71), (36), (26), (24), (23), (17), (13), (8), (4), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN119307) Images
Specificity This antibody reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein. Clone HDC8A4 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. Clone HDC8A4 recognises both denatured and native huntingtin in human brain. The combined use of clone HDC8A4 (SM1662), HDB4E10 (SM1661) and HDA3E10 (SM1660) demonstrate that huntingtin is enriched in neuronal cells in the brain.
Purification Affinity chromatography on Protein G
Immunogen Recombinant protein corresponding to amino acids 2703 - 2911 of huntingtin
Clone HDC8A4
Isotype IgG1

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling References for anti-Huntingtin Antibody (ABIN119307) Images back to top
Antigen
Alternative Name Huntingtin (HTT Antibody Abstract)
Background Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegeneative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years. The HD gene product is widely expressed in human tissues, with the highest level of expression in the brain. The huntingtin gene product is expressed at similar levels in patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using yeast two-hybrid system, HAP1 (huntingtin associated protein 1) has been identified, that associates with huntingtin protein. The In vitro data suggest that the association between HAP1 and huntingtin is enhanced by increasing length of glutamine repeat.Synonyms: HD, HTT, Huntington Disease Protein, IT15
Gene ID 3064
NCBI Accession NP_002102
UniProt P42858
Research Area Neurology, Virology
Pathways PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling References for anti-Huntingtin Antibody (ABIN119307) Images back to top
Application Notes Immunohistochemistry on frozen sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details References for anti-Huntingtin Antibody (ABIN119307) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Huntingtin Antibody (ABIN119307)

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling Images back to top
Background publications

Wilkinson, Nguyen, Manilal, Thomas, Neal, Harper, Jones, Morris: "Localization of rabbit huntingtin using a new panel of monoclonal antibodies." in: Brain research. Molecular brain research, Vol. 69, Issue 1, pp. 10-20, 1999

Images

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling References for anti-Huntingtin Antibody (ABIN119307) back to top
Supplier Images
Western Blotting (WB) image for anti-Huntingtin antibody (HTT) (AA 2703-2911) (ABIN119307) Total protein extract of normal human cerebral cortex separated as a strip on a 3-12....